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Standardization of Antigen-Emulsion Preparations for the Induction of Autoimmune Disease Models

Topping, Louise M. ; Romero-Castillo, Laura ; Urbonaviciute, Vilma ; Bolinsson, Hans LU orcid ; Clanchy, Felix I. ; Holmdahl, Rikard LU ; Bäckström, B. Thomas LU and Williams, Richard O. (2022) In Frontiers in Immunology 13.
Abstract

Autoimmune murine disease models are vital tools for identifying novel targets and finding better treatments for human diseases. Complete Freund’s adjuvant is commonly used to induce disease in autoimmune models, and the quality of the adjuvant/autoantigen emulsion is of critical importance in determining reproducibility. We have established an emulsification method using a standard homogenizer and specially designed receptacle. Emulsions are easy to prepare, form stable and uniform water-in-oil particles, are faster to make than the traditional syringe method, use less material and are designed to fill syringes with ease. In the present study, we have validated the emulsions for induction of experimental autoimmune encephalitis,... (More)

Autoimmune murine disease models are vital tools for identifying novel targets and finding better treatments for human diseases. Complete Freund’s adjuvant is commonly used to induce disease in autoimmune models, and the quality of the adjuvant/autoantigen emulsion is of critical importance in determining reproducibility. We have established an emulsification method using a standard homogenizer and specially designed receptacle. Emulsions are easy to prepare, form stable and uniform water-in-oil particles, are faster to make than the traditional syringe method, use less material and are designed to fill syringes with ease. In the present study, we have validated the emulsions for induction of experimental autoimmune encephalitis, collagen II induced arthritis, antigen induced arthritis, and delayed type hypersensitivity models. These models were induced consistently and reproducibly and, in some cases, the new method outperformed the traditional method. The method described herein is simple, cost-effective and will reduce variability, thereby requiring fewer animals for in vivo research involving animal models of autoimmune disease and in vaccine development.

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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
animal disease models, antigen emulsions, autoimmunity, CIA, Complete Freund’s Adjuvant, EAE, emulsification
in
Frontiers in Immunology
volume
13
article number
892251
publisher
Frontiers Media S. A.
external identifiers
  • scopus:85133147317
  • pmid:35769487
ISSN
1664-3224
DOI
10.3389/fimmu.2022.892251
language
English
LU publication?
yes
id
d1827570-0ad0-482f-bb9a-1d43a43b7fd8
date added to LUP
2022-09-29 15:15:13
date last changed
2025-03-22 00:15:36
@article{d1827570-0ad0-482f-bb9a-1d43a43b7fd8,
  abstract     = {{<p>Autoimmune murine disease models are vital tools for identifying novel targets and finding better treatments for human diseases. Complete Freund’s adjuvant is commonly used to induce disease in autoimmune models, and the quality of the adjuvant/autoantigen emulsion is of critical importance in determining reproducibility. We have established an emulsification method using a standard homogenizer and specially designed receptacle. Emulsions are easy to prepare, form stable and uniform water-in-oil particles, are faster to make than the traditional syringe method, use less material and are designed to fill syringes with ease. In the present study, we have validated the emulsions for induction of experimental autoimmune encephalitis, collagen II induced arthritis, antigen induced arthritis, and delayed type hypersensitivity models. These models were induced consistently and reproducibly and, in some cases, the new method outperformed the traditional method. The method described herein is simple, cost-effective and will reduce variability, thereby requiring fewer animals for in vivo research involving animal models of autoimmune disease and in vaccine development.</p>}},
  author       = {{Topping, Louise M. and Romero-Castillo, Laura and Urbonaviciute, Vilma and Bolinsson, Hans and Clanchy, Felix I. and Holmdahl, Rikard and Bäckström, B. Thomas and Williams, Richard O.}},
  issn         = {{1664-3224}},
  keywords     = {{animal disease models; antigen emulsions; autoimmunity; CIA; Complete Freund’s Adjuvant; EAE; emulsification}},
  language     = {{eng}},
  month        = {{06}},
  publisher    = {{Frontiers Media S. A.}},
  series       = {{Frontiers in Immunology}},
  title        = {{Standardization of Antigen-Emulsion Preparations for the Induction of Autoimmune Disease Models}},
  url          = {{http://dx.doi.org/10.3389/fimmu.2022.892251}},
  doi          = {{10.3389/fimmu.2022.892251}},
  volume       = {{13}},
  year         = {{2022}},
}