Development of an experimental ex vivo wound model to evaluate antimicrobial efficacy of topical formulations
(2021) In International Journal of Molecular Sciences 22(9).- Abstract
Wound infections are considered a major cause for wound-associated morbidity. There is a high demand for alternative, robust, and affordable methods that can provide relatable and repro-ducible results when testing topical treatments, both in research and in the pharmaceutical industry. Here we present an ex vivo wound infection model using porcine skin and a burn wounding method, allowing for the efficacy evaluation of topical antimicrobial formulations. Utilizing this model, we demonstrate the potential of topical treatments after infecting the wounds with clinically significant bacteria, P. aeruginosa and S. aureus. We show that the method is compatible with several analytical tools used to analyze infection and antimicrobial... (More)
Wound infections are considered a major cause for wound-associated morbidity. There is a high demand for alternative, robust, and affordable methods that can provide relatable and repro-ducible results when testing topical treatments, both in research and in the pharmaceutical industry. Here we present an ex vivo wound infection model using porcine skin and a burn wounding method, allowing for the efficacy evaluation of topical antimicrobial formulations. Utilizing this model, we demonstrate the potential of topical treatments after infecting the wounds with clinically significant bacteria, P. aeruginosa and S. aureus. We show that the method is compatible with several analytical tools used to analyze infection and antimicrobial effects. Both bacterial strains success-fully infected the wound surface, as well as deeper regions of the tissue. Quantification of viable bacteria on the wound surface and in the tissue, longitudinal measurements of bioluminescence, fluorescence microscopy, and scanning electron microscopy were used to confirm the effects of an-tibacterial treatments. Furthermore, we show that biofilms are formed on the wound surface, indi-cating that the demonstrated method mirrors typical in vivo infections.
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- author
- Andersson, Madelene ; Madsen, Lone Bruhn ; Schmidtchen, Artur LU and Puthia, Manoj LU
- organization
- publishing date
- 2021-05-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Antibiotics, Antimicrobial, Biofilm, Burn, Ex vivo, Wound healing, Wound model
- in
- International Journal of Molecular Sciences
- volume
- 22
- issue
- 9
- article number
- 5045
- publisher
- MDPI AG
- external identifiers
-
- pmid:34068733
- scopus:85105458936
- ISSN
- 1661-6596
- DOI
- 10.3390/ijms22095045
- language
- English
- LU publication?
- yes
- id
- d1c711af-033e-4695-b3ab-042024edbb39
- date added to LUP
- 2021-06-03 15:29:31
- date last changed
- 2024-06-15 12:05:22
@article{d1c711af-033e-4695-b3ab-042024edbb39,
abstract = {{<p>Wound infections are considered a major cause for wound-associated morbidity. There is a high demand for alternative, robust, and affordable methods that can provide relatable and repro-ducible results when testing topical treatments, both in research and in the pharmaceutical industry. Here we present an ex vivo wound infection model using porcine skin and a burn wounding method, allowing for the efficacy evaluation of topical antimicrobial formulations. Utilizing this model, we demonstrate the potential of topical treatments after infecting the wounds with clinically significant bacteria, P. aeruginosa and S. aureus. We show that the method is compatible with several analytical tools used to analyze infection and antimicrobial effects. Both bacterial strains success-fully infected the wound surface, as well as deeper regions of the tissue. Quantification of viable bacteria on the wound surface and in the tissue, longitudinal measurements of bioluminescence, fluorescence microscopy, and scanning electron microscopy were used to confirm the effects of an-tibacterial treatments. Furthermore, we show that biofilms are formed on the wound surface, indi-cating that the demonstrated method mirrors typical in vivo infections.</p>}},
author = {{Andersson, Madelene and Madsen, Lone Bruhn and Schmidtchen, Artur and Puthia, Manoj}},
issn = {{1661-6596}},
keywords = {{Antibiotics; Antimicrobial; Biofilm; Burn; Ex vivo; Wound healing; Wound model}},
language = {{eng}},
month = {{05}},
number = {{9}},
publisher = {{MDPI AG}},
series = {{International Journal of Molecular Sciences}},
title = {{Development of an experimental ex vivo wound model to evaluate antimicrobial efficacy of topical formulations}},
url = {{http://dx.doi.org/10.3390/ijms22095045}},
doi = {{10.3390/ijms22095045}},
volume = {{22}},
year = {{2021}},
}