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The NF-κB-modulated miR-19a-3p enhances malignancy of human ovarian cancer cells through inhibition of IGFBP-3 expression

Bai, Ru ; Cui, Zhenhua ; Ma, Yongjing ; Wu, Yang ; Wang, Ningping ; Huang, Ling ; Yao, Qing and Sun, Jianmin LU (2019) In Molecular Carcinogenesis 58(12). p.2254-2265
Abstract

Ovarian cancer is the most lethal gynecologic malignancy due to the lack of symptoms until advanced stages, and new diagnosis and treatment strategy is in urgent need. In this study, we found higher expression of miR-19a-3p in ovarian cancer tissues compared with that in the adjacent normal tissues. By chromatin immunoprecipitation (ChIP) and electrophoretic mobility shift assay (EMSA) analysis, we showed that nuclear factor-kappaB (NF-κB) binds to the promoter of miR-19a-3p, leading to reduced expression in ovarian cancer cells. Further study indicated that miR-19a-3p inhibits the expression of insulin-like growth factor binding protein-3 (IGFBP-3), resulting in enhanced growth and migration of ovarian cancer cells in vitro and tumor... (More)

Ovarian cancer is the most lethal gynecologic malignancy due to the lack of symptoms until advanced stages, and new diagnosis and treatment strategy is in urgent need. In this study, we found higher expression of miR-19a-3p in ovarian cancer tissues compared with that in the adjacent normal tissues. By chromatin immunoprecipitation (ChIP) and electrophoretic mobility shift assay (EMSA) analysis, we showed that nuclear factor-kappaB (NF-κB) binds to the promoter of miR-19a-3p, leading to reduced expression in ovarian cancer cells. Further study indicated that miR-19a-3p inhibits the expression of insulin-like growth factor binding protein-3 (IGFBP-3), resulting in enhanced growth and migration of ovarian cancer cells in vitro and tumor growth in vivo. These results showed that miR-19a-3p enhances the oncogenesis of ovarian cancer through inhibition of IGFBP-3 expression, and which can be inhibited by NF-κB, suggesting an NF-κB/miR-19a-3p/IGFBP-3 pathway in the oncogenesis of ovarian cancer, which expands our understanding of ovarian cancer and they may contribute to the development of new diagnosis and treatment of ovarian cancer.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
IGFBP-3, miR-19a-3p, NF-κB, ovarian cancer
in
Molecular Carcinogenesis
volume
58
issue
12
pages
12 pages
publisher
John Wiley & Sons
external identifiers
  • scopus:85073789803
  • pmid:31513316
ISSN
0899-1987
DOI
10.1002/mc.23113
language
English
LU publication?
yes
id
d3234bd5-92c8-4cd5-9655-d1551f4af997
date added to LUP
2019-11-01 11:30:50
date last changed
2020-02-19 05:41:20
@article{d3234bd5-92c8-4cd5-9655-d1551f4af997,
  abstract     = {<p>Ovarian cancer is the most lethal gynecologic malignancy due to the lack of symptoms until advanced stages, and new diagnosis and treatment strategy is in urgent need. In this study, we found higher expression of miR-19a-3p in ovarian cancer tissues compared with that in the adjacent normal tissues. By chromatin immunoprecipitation (ChIP) and electrophoretic mobility shift assay (EMSA) analysis, we showed that nuclear factor-kappaB (NF-κB) binds to the promoter of miR-19a-3p, leading to reduced expression in ovarian cancer cells. Further study indicated that miR-19a-3p inhibits the expression of insulin-like growth factor binding protein-3 (IGFBP-3), resulting in enhanced growth and migration of ovarian cancer cells in vitro and tumor growth in vivo. These results showed that miR-19a-3p enhances the oncogenesis of ovarian cancer through inhibition of IGFBP-3 expression, and which can be inhibited by NF-κB, suggesting an NF-κB/miR-19a-3p/IGFBP-3 pathway in the oncogenesis of ovarian cancer, which expands our understanding of ovarian cancer and they may contribute to the development of new diagnosis and treatment of ovarian cancer.</p>},
  author       = {Bai, Ru and Cui, Zhenhua and Ma, Yongjing and Wu, Yang and Wang, Ningping and Huang, Ling and Yao, Qing and Sun, Jianmin},
  issn         = {0899-1987},
  language     = {eng},
  number       = {12},
  pages        = {2254--2265},
  publisher    = {John Wiley & Sons},
  series       = {Molecular Carcinogenesis},
  title        = {The NF-κB-modulated miR-19a-3p enhances malignancy of human ovarian cancer cells through inhibition of IGFBP-3 expression},
  url          = {http://dx.doi.org/10.1002/mc.23113},
  doi          = {10.1002/mc.23113},
  volume       = {58},
  year         = {2019},
}