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Species‐ and organ‐specificity of secretory proteins derived from human prostate and seminal vesicles

Aumüller, G. ; Seitz, J. ; Lilja, H. LU orcid ; Abrahamsson, P. ‐A LU ; Von Kammer, H. Der and Scheit, K. ‐H (1990) In The Prostate 17(1). p.31-40
Abstract

Polyclonal antibodies against semenogelin (SG) isolated from human seminal vesicle secretion and acid phosphatase (PAP), β‐microseminoprotein (β‐MSP), and Prostate‐Specific Antigen (PSA) derived from human prostatic fluid, as well as a monoclonal antibody against β‐MSP were used for immunocytochemical detection of the respective antigens in different organs from different species. SG immunoreactivity was detected in the epithelium of the pubertal and adult human and in monkey seminal vesicle, ampulla of the vas deferens, and ejaculatory duct. PAP, β‐MSP, and PSA immunoreactivities were detected in the pubertal and adult human prostate and the cranial and caudal monkey prostate. With the exception of a weak PSA immunoreactivity in the... (More)

Polyclonal antibodies against semenogelin (SG) isolated from human seminal vesicle secretion and acid phosphatase (PAP), β‐microseminoprotein (β‐MSP), and Prostate‐Specific Antigen (PSA) derived from human prostatic fluid, as well as a monoclonal antibody against β‐MSP were used for immunocytochemical detection of the respective antigens in different organs from different species. SG immunoreactivity was detected in the epithelium of the pubertal and adult human and in monkey seminal vesicle, ampulla of the vas deferens, and ejaculatory duct. PAP, β‐MSP, and PSA immunoreactivities were detected in the pubertal and adult human prostate and the cranial and caudal monkey prostate. With the exception of a weak PSA immunoreactivity in the proximal portions of the ejaculatory duct, none of the latter antisera reacted with seminal vesicle, ampullary, and ejaculatory duct epithelium. Among the non‐primate species studied (dog, bull, rat, guinea pig) only the canine prostatic epithelium displayed a definite immunoreactivity with the PAP antibody and a moderate reaction with the PSA antibody. No immunoreaction was seen in bull and rat seminal vesicle and canine ampulla of the vas deferens with the SG antibody. The same was true for the (ventral) prostate of rat, bull, and dog for β‐MSP. The epithelium of the rat dorsal prostate showed a slight cross‐reactivity with the monoclonal antibody against β‐MSP and one polyclonal antibody against PSA. The findings indicate a rather strict species‐dependent expression of human seminal proteins which show some similarities in primates, but only marginal relationship to species with different physiology of seminal fluid.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
ampulla of vas deferens, carnivores, ejaculatory ducts, immunocytochemistry, primates, rodents
in
The Prostate
volume
17
issue
1
pages
31 - 40
publisher
John Wiley & Sons Inc.
external identifiers
  • pmid:1696713
  • scopus:0025160687
ISSN
0270-4137
DOI
10.1002/pros.2990170105
language
English
LU publication?
yes
id
d347cdb4-8e18-46b4-8d24-6e2858265652
date added to LUP
2022-12-17 17:06:12
date last changed
2024-01-03 11:01:57
@article{d347cdb4-8e18-46b4-8d24-6e2858265652,
  abstract     = {{<p>Polyclonal antibodies against semenogelin (SG) isolated from human seminal vesicle secretion and acid phosphatase (PAP), β‐microseminoprotein (β‐MSP), and Prostate‐Specific Antigen (PSA) derived from human prostatic fluid, as well as a monoclonal antibody against β‐MSP were used for immunocytochemical detection of the respective antigens in different organs from different species. SG immunoreactivity was detected in the epithelium of the pubertal and adult human and in monkey seminal vesicle, ampulla of the vas deferens, and ejaculatory duct. PAP, β‐MSP, and PSA immunoreactivities were detected in the pubertal and adult human prostate and the cranial and caudal monkey prostate. With the exception of a weak PSA immunoreactivity in the proximal portions of the ejaculatory duct, none of the latter antisera reacted with seminal vesicle, ampullary, and ejaculatory duct epithelium. Among the non‐primate species studied (dog, bull, rat, guinea pig) only the canine prostatic epithelium displayed a definite immunoreactivity with the PAP antibody and a moderate reaction with the PSA antibody. No immunoreaction was seen in bull and rat seminal vesicle and canine ampulla of the vas deferens with the SG antibody. The same was true for the (ventral) prostate of rat, bull, and dog for β‐MSP. The epithelium of the rat dorsal prostate showed a slight cross‐reactivity with the monoclonal antibody against β‐MSP and one polyclonal antibody against PSA. The findings indicate a rather strict species‐dependent expression of human seminal proteins which show some similarities in primates, but only marginal relationship to species with different physiology of seminal fluid.</p>}},
  author       = {{Aumüller, G. and Seitz, J. and Lilja, H. and Abrahamsson, P. ‐A and Von Kammer, H. Der and Scheit, K. ‐H}},
  issn         = {{0270-4137}},
  keywords     = {{ampulla of vas deferens; carnivores; ejaculatory ducts; immunocytochemistry; primates; rodents}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{31--40}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{The Prostate}},
  title        = {{Species‐ and organ‐specificity of secretory proteins derived from human prostate and seminal vesicles}},
  url          = {{http://dx.doi.org/10.1002/pros.2990170105}},
  doi          = {{10.1002/pros.2990170105}},
  volume       = {{17}},
  year         = {{1990}},
}