Advanced

A soft tick Ornithodoros moubata salivary protein OmCI is a potent inhibitor to prevent avian complement activation

Frye, Amber M. ; Hart, Thomas M. ; Tufts, Danielle M. ; Ram, Sanjay ; Diuk-Wasser, Maria A. ; Kraiczy, Peter ; Blom, Anna M. LU and Lin, Yi Pin (2020) In Ticks and Tick-borne Diseases 11(2).
Abstract

Complement is a key first line innate host defense system in the blood of vertebrates. Upon activation, this powerful defense mechanism can elicit inflammatory responses, lyse non-self-cells, or mark them for opsonophagocytic removal. Blood-feeding arthropods thus require the ability to block host complement activation in the bloodmeal to prevent undesired cell or tissue damage during feeding. The soft tick Ornithodoros moubata produces a complement inhibitory protein, OmCI. This protein binds to a mammalian complement protein C5 and blocks further activation of complement cascades, which results in the prevention of complement-mediated bacterial killing through membrane attack complex. Interestingly, the amino acids involved in OmCI... (More)

Complement is a key first line innate host defense system in the blood of vertebrates. Upon activation, this powerful defense mechanism can elicit inflammatory responses, lyse non-self-cells, or mark them for opsonophagocytic removal. Blood-feeding arthropods thus require the ability to block host complement activation in the bloodmeal to prevent undesired cell or tissue damage during feeding. The soft tick Ornithodoros moubata produces a complement inhibitory protein, OmCI. This protein binds to a mammalian complement protein C5 and blocks further activation of complement cascades, which results in the prevention of complement-mediated bacterial killing through membrane attack complex. Interestingly, the amino acids involved in OmCI binding are highly conserved among mammalian and avian C5, but the ability of this protein to inhibit the complement from birds remains unclear. Here we demonstrated that OmCI is capable of preventing quail complement-mediated erythrocyte lysis, inhibiting the capability of this animal's complement to eliminate a serum-sensitive Lyme disease bacterial strain. We also found that the ability of OmCI to inhibit quail complement-mediated killing of Lyme disease bacteria can be extended to different domestic and wild birds. Our results illustrate the utility of OmCI to block bird complement. These results provide the foundation for further use of this protein as a tool to study the molecular basis of avian complement and pathogen evasion to such a defense mechanism.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Avian complement, Bacterial killing, Lyme borreliae, OmCI
in
Ticks and Tick-borne Diseases
volume
11
issue
2
article number
101354
publisher
Elsevier
external identifiers
  • pmid:31866440
  • scopus:85076845488
ISSN
1877-959X
DOI
10.1016/j.ttbdis.2019.101354
language
English
LU publication?
yes
id
d41dba78-e760-451f-86da-31a75fc90ea5
date added to LUP
2020-01-10 13:29:56
date last changed
2020-10-24 02:18:04
@article{d41dba78-e760-451f-86da-31a75fc90ea5,
  abstract     = {<p>Complement is a key first line innate host defense system in the blood of vertebrates. Upon activation, this powerful defense mechanism can elicit inflammatory responses, lyse non-self-cells, or mark them for opsonophagocytic removal. Blood-feeding arthropods thus require the ability to block host complement activation in the bloodmeal to prevent undesired cell or tissue damage during feeding. The soft tick Ornithodoros moubata produces a complement inhibitory protein, OmCI. This protein binds to a mammalian complement protein C5 and blocks further activation of complement cascades, which results in the prevention of complement-mediated bacterial killing through membrane attack complex. Interestingly, the amino acids involved in OmCI binding are highly conserved among mammalian and avian C5, but the ability of this protein to inhibit the complement from birds remains unclear. Here we demonstrated that OmCI is capable of preventing quail complement-mediated erythrocyte lysis, inhibiting the capability of this animal's complement to eliminate a serum-sensitive Lyme disease bacterial strain. We also found that the ability of OmCI to inhibit quail complement-mediated killing of Lyme disease bacteria can be extended to different domestic and wild birds. Our results illustrate the utility of OmCI to block bird complement. These results provide the foundation for further use of this protein as a tool to study the molecular basis of avian complement and pathogen evasion to such a defense mechanism.</p>},
  author       = {Frye, Amber M. and Hart, Thomas M. and Tufts, Danielle M. and Ram, Sanjay and Diuk-Wasser, Maria A. and Kraiczy, Peter and Blom, Anna M. and Lin, Yi Pin},
  issn         = {1877-959X},
  language     = {eng},
  number       = {2},
  publisher    = {Elsevier},
  series       = {Ticks and Tick-borne Diseases},
  title        = {A soft tick Ornithodoros moubata salivary protein OmCI is a potent inhibitor to prevent avian complement activation},
  url          = {http://dx.doi.org/10.1016/j.ttbdis.2019.101354},
  doi          = {10.1016/j.ttbdis.2019.101354},
  volume       = {11},
  year         = {2020},
}