Detailed characterization of plasmids carrying resistance genes using optical DNA mapping
(2016) 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 p.1561-1562- Abstract
We present an assay, based on optical DNA mapping in nanochannels that is capable of characterizing the plasmid content of bacterial isolates resistant to antibiotics in a fast an detailed way. In a single experiment we determine the number of different plasmids in each sample, their size, as well as a barcode that can be used for plasmid identification and tracing. In addition we demonstrate that we can identify resistance genes on individual plasmids using CRISPR/Cas9. We foresee that the assay can be a useful tool all the way from fundamental plasmid biology to diagnostics and surveillance of resistant infections.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/d446b5e3-66ab-4c12-86b9-e88fd363ba7b
- author
- Müller, Vilhelm ; Nyberg, Lena K. ; Quaderi, Saair ; Kristiansson, Erik ; Ambjörnsson, Tobias LU ; Sandegren, Linus and Westerlund, Fredrik
- organization
- publishing date
- 2016
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Antibiotic resistance, CRISPR/Cas9, Nanofluidics, Optical DNA mapping, Plasmids
- host publication
- 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
- pages
- 2 pages
- publisher
- Chemical and Biological Microsystems Society
- conference name
- 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
- conference location
- Dublin, Ireland
- conference dates
- 2016-10-09 - 2016-10-13
- external identifiers
-
- scopus:85014171223
- ISBN
- 9780979806490
- language
- English
- LU publication?
- yes
- id
- d446b5e3-66ab-4c12-86b9-e88fd363ba7b
- date added to LUP
- 2017-03-16 10:36:17
- date last changed
- 2024-01-13 17:16:32
@inproceedings{d446b5e3-66ab-4c12-86b9-e88fd363ba7b, abstract = {{<p>We present an assay, based on optical DNA mapping in nanochannels that is capable of characterizing the plasmid content of bacterial isolates resistant to antibiotics in a fast an detailed way. In a single experiment we determine the number of different plasmids in each sample, their size, as well as a barcode that can be used for plasmid identification and tracing. In addition we demonstrate that we can identify resistance genes on individual plasmids using CRISPR/Cas9. We foresee that the assay can be a useful tool all the way from fundamental plasmid biology to diagnostics and surveillance of resistant infections.</p>}}, author = {{Müller, Vilhelm and Nyberg, Lena K. and Quaderi, Saair and Kristiansson, Erik and Ambjörnsson, Tobias and Sandegren, Linus and Westerlund, Fredrik}}, booktitle = {{20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016}}, isbn = {{9780979806490}}, keywords = {{Antibiotic resistance; CRISPR/Cas9; Nanofluidics; Optical DNA mapping; Plasmids}}, language = {{eng}}, pages = {{1561--1562}}, publisher = {{Chemical and Biological Microsystems Society}}, title = {{Detailed characterization of plasmids carrying resistance genes using optical DNA mapping}}, year = {{2016}}, }