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Detailed characterization of plasmids carrying resistance genes using optical DNA mapping

Müller, Vilhelm; Nyberg, Lena K.; Quaderi, Saair; Kristiansson, Erik; Ambjörnsson, Tobias LU ; Sandegren, Linus and Westerlund, Fredrik (2016) 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 p.1561-1562
Abstract

We present an assay, based on optical DNA mapping in nanochannels that is capable of characterizing the plasmid content of bacterial isolates resistant to antibiotics in a fast an detailed way. In a single experiment we determine the number of different plasmids in each sample, their size, as well as a barcode that can be used for plasmid identification and tracing. In addition we demonstrate that we can identify resistance genes on individual plasmids using CRISPR/Cas9. We foresee that the assay can be a useful tool all the way from fundamental plasmid biology to diagnostics and surveillance of resistant infections.

Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Antibiotic resistance, CRISPR/Cas9, Nanofluidics, Optical DNA mapping, Plasmids
host publication
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
pages
2 pages
publisher
Chemical and Biological Microsystems Society
conference name
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
conference location
Dublin, Ireland
conference dates
2016-10-09 - 2016-10-13
external identifiers
  • scopus:85014171223
ISBN
9780979806490
language
English
LU publication?
yes
id
d446b5e3-66ab-4c12-86b9-e88fd363ba7b
date added to LUP
2017-03-16 10:36:17
date last changed
2019-02-20 10:29:15
@inproceedings{d446b5e3-66ab-4c12-86b9-e88fd363ba7b,
  abstract     = {<p>We present an assay, based on optical DNA mapping in nanochannels that is capable of characterizing the plasmid content of bacterial isolates resistant to antibiotics in a fast an detailed way. In a single experiment we determine the number of different plasmids in each sample, their size, as well as a barcode that can be used for plasmid identification and tracing. In addition we demonstrate that we can identify resistance genes on individual plasmids using CRISPR/Cas9. We foresee that the assay can be a useful tool all the way from fundamental plasmid biology to diagnostics and surveillance of resistant infections.</p>},
  author       = {Müller, Vilhelm and Nyberg, Lena K. and Quaderi, Saair and Kristiansson, Erik and Ambjörnsson, Tobias and Sandegren, Linus and Westerlund, Fredrik},
  isbn         = {9780979806490},
  keyword      = {Antibiotic resistance,CRISPR/Cas9,Nanofluidics,Optical DNA mapping,Plasmids},
  language     = {eng},
  location     = {Dublin, Ireland},
  pages        = {1561--1562},
  publisher    = {Chemical and Biological Microsystems Society},
  title        = {Detailed characterization of plasmids carrying resistance genes using optical DNA mapping},
  year         = {2016},
}