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Detailed characterization of plasmids carrying resistance genes using optical DNA mapping

Müller, Vilhelm; Nyberg, Lena K.; Quaderi, Saair; Kristiansson, Erik; Ambjörnsson, Tobias LU ; Sandegren, Linus and Westerlund, Fredrik (2016) 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 p.1561-1562
Abstract

We present an assay, based on optical DNA mapping in nanochannels that is capable of characterizing the plasmid content of bacterial isolates resistant to antibiotics in a fast an detailed way. In a single experiment we determine the number of different plasmids in each sample, their size, as well as a barcode that can be used for plasmid identification and tracing. In addition we demonstrate that we can identify resistance genes on individual plasmids using CRISPR/Cas9. We foresee that the assay can be a useful tool all the way from fundamental plasmid biology to diagnostics and surveillance of resistant infections.

Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Antibiotic resistance, CRISPR/Cas9, Nanofluidics, Optical DNA mapping, Plasmids
in
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
pages
2 pages
publisher
Chemical and Biological Microsystems Society
conference name
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
external identifiers
  • scopus:85014171223
ISBN
9780979806490
language
English
LU publication?
yes
id
d446b5e3-66ab-4c12-86b9-e88fd363ba7b
date added to LUP
2017-03-16 10:36:17
date last changed
2017-03-20 09:48:09
@inproceedings{d446b5e3-66ab-4c12-86b9-e88fd363ba7b,
  abstract     = {<p>We present an assay, based on optical DNA mapping in nanochannels that is capable of characterizing the plasmid content of bacterial isolates resistant to antibiotics in a fast an detailed way. In a single experiment we determine the number of different plasmids in each sample, their size, as well as a barcode that can be used for plasmid identification and tracing. In addition we demonstrate that we can identify resistance genes on individual plasmids using CRISPR/Cas9. We foresee that the assay can be a useful tool all the way from fundamental plasmid biology to diagnostics and surveillance of resistant infections.</p>},
  author       = {Müller, Vilhelm and Nyberg, Lena K. and Quaderi, Saair and Kristiansson, Erik and Ambjörnsson, Tobias and Sandegren, Linus and Westerlund, Fredrik},
  booktitle    = {20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016},
  isbn         = {9780979806490},
  keyword      = {Antibiotic resistance,CRISPR/Cas9,Nanofluidics,Optical DNA mapping,Plasmids},
  language     = {eng},
  pages        = {1561--1562},
  publisher    = {Chemical and Biological Microsystems Society},
  title        = {Detailed characterization of plasmids carrying resistance genes using optical DNA mapping},
  year         = {2016},
}