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Radiopaque dyes allow vessel imaging in lung tissue using laboratory phase contrast micro-CT

Dreier, Till LU orcid ; Bernström, Gustaf ; Ganji, Sahel ; Norvik, Christian LU ; Tran Lundmark, Karin LU and Bech, Martin LU orcid (2022) Developments in X-Ray Tomography XIV 2022 12242.
Abstract
Phase contrast micro-CT is a powerful technique allowing imaging of soft tissue at synchrotrons or using lab- oratory sources. The use of contrast agents is a useful approach when imaging vascular structures. However, common x-ray contrast agents typically rely on heavy metals to increase absorption, which may affect the phase contrast and cause artifacts in the reconstructed volumes. Thus, utilizing an agent with lower attenuation similar to soft tissue is clearly advantageous. Here, we evaluated different colored radiopaque solutions (tissue marking dyes) which had been injected into the vascular system of bovine lung samples, prior to embedding in paraffin. Scans were performed using a micro-focus x-tube calibrated to 10 µm spot size at... (More)
Phase contrast micro-CT is a powerful technique allowing imaging of soft tissue at synchrotrons or using lab- oratory sources. The use of contrast agents is a useful approach when imaging vascular structures. However, common x-ray contrast agents typically rely on heavy metals to increase absorption, which may affect the phase contrast and cause artifacts in the reconstructed volumes. Thus, utilizing an agent with lower attenuation similar to soft tissue is clearly advantageous. Here, we evaluated different colored radiopaque solutions (tissue marking dyes) which had been injected into the vascular system of bovine lung samples, prior to embedding in paraffin. Scans were performed using a micro-focus x-tube calibrated to 10 µm spot size at 70 kV and a photon counting detector with a silicon sensor and 75 µm pixels. The resulting volumes have a voxel size of (25 µm)3, limited by the size of the samples, but sufficient to resolve the vascular system. Experiments confirmed that sufficient perfusion of the vessels with the dyes could be achieved, and the different dyes could be clearly discerned in the reconstructed volumes without causing artifacts allowing to clearly identify features in the soft tissue. Further, the findings were confirmed by histology.
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author
; ; ; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
host publication
Proceedings of SPIE - The International Society for Optical Engineering
editor
Müller, Bert and Wang, Ge
volume
12242
article number
1224203
publisher
SPIE
conference name
Developments in X-Ray Tomography XIV 2022
conference location
San Diego, United States
conference dates
2022-08-22 - 2022-08-24
external identifiers
  • scopus:85184839203
DOI
10.1117/12.2632343
project
Industrial PhD student
language
English
LU publication?
yes
id
d5400ce3-5dd1-493d-92de-ca52f9a46009
date added to LUP
2022-10-18 14:27:20
date last changed
2024-02-27 10:58:19
@inproceedings{d5400ce3-5dd1-493d-92de-ca52f9a46009,
  abstract     = {{Phase contrast micro-CT is a powerful technique allowing imaging of soft tissue at synchrotrons or using lab- oratory sources. The use of contrast agents is a useful approach when imaging vascular structures. However, common x-ray contrast agents typically rely on heavy metals to increase absorption, which may affect the phase contrast and cause artifacts in the reconstructed volumes. Thus, utilizing an agent with lower attenuation similar to soft tissue is clearly advantageous. Here, we evaluated different colored radiopaque solutions (tissue marking dyes) which had been injected into the vascular system of bovine lung samples, prior to embedding in paraffin. Scans were performed using a micro-focus x-tube calibrated to 10 µm spot size at 70 kV and a photon counting detector with a silicon sensor and 75 µm pixels. The resulting volumes have a voxel size of (25 µm)3, limited by the size of the samples, but sufficient to resolve the vascular system. Experiments confirmed that sufficient perfusion of the vessels with the dyes could be achieved, and the different dyes could be clearly discerned in the reconstructed volumes without causing artifacts allowing to clearly identify features in the soft tissue. Further, the findings were confirmed by histology.<br/>Keywords:}},
  author       = {{Dreier, Till and Bernström, Gustaf and Ganji, Sahel and Norvik, Christian and Tran Lundmark, Karin and Bech, Martin}},
  booktitle    = {{Proceedings of SPIE - The International Society for Optical Engineering}},
  editor       = {{Müller, Bert and Wang, Ge}},
  language     = {{eng}},
  month        = {{10}},
  publisher    = {{SPIE}},
  title        = {{Radiopaque dyes allow vessel imaging in lung tissue using laboratory phase contrast micro-CT}},
  url          = {{http://dx.doi.org/10.1117/12.2632343}},
  doi          = {{10.1117/12.2632343}},
  volume       = {{12242}},
  year         = {{2022}},
}