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Three-dimensional scaffolds of acellular human and porcine lungs for high throughput studies of lung disease and regeneration

Wagner, Darcy E. LU ; Bonenfant, Nicholas R.; Sokocevic, Dino; DeSarno, Michael J; Borg, Zachary D.; Parsons, Charles S.; Brooks, Elice M.; Platz, Joseph; Khalpey, Zain I. and Hoganson, David M., et al. (2014) In Biomaterials 35(9). p.2664-2679
Abstract

Acellular scaffolds from complex whole organs such as lung are being increasingly studied for ex vivo organ generation and for in vitro studies of cell-extracellular matrix interactions. We have established effective methods for efficient de and recellularization of large animal and human lungs including techniques which allow multiple small segments (~1-3 cm3) to be excised that retain 3-dimensional lung structure. Coupled with the use of a synthetic pleural coating, cells can be selectively physiologically inoculated via preserved vascular and airway conduits. Inoculated segments can be further sliced for high throughput studies. Further, we demonstrate thermography as a powerful noninvasive technique for monitoring... (More)

Acellular scaffolds from complex whole organs such as lung are being increasingly studied for ex vivo organ generation and for in vitro studies of cell-extracellular matrix interactions. We have established effective methods for efficient de and recellularization of large animal and human lungs including techniques which allow multiple small segments (~1-3 cm3) to be excised that retain 3-dimensional lung structure. Coupled with the use of a synthetic pleural coating, cells can be selectively physiologically inoculated via preserved vascular and airway conduits. Inoculated segments can be further sliced for high throughput studies. Further, we demonstrate thermography as a powerful noninvasive technique for monitoring perfusion decellularization and for evaluating preservation of vascular and airway networks following human and porcine lung decellularization. Collectively, these techniques are a significant step forward as they allow high throughput in vitro studies from a single lung or lobe in a more biologically relevant, three-dimensional acellular scaffold.

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type
Contribution to journal
publication status
published
keywords
Acellular matrix, Endothelial cell, Epithelial cell, Extracellular matrix (ECM), Human lung fibroblast, Mesenchymal stem cell
in
Biomaterials
volume
35
issue
9
pages
2664 - 2679
publisher
Elsevier
external identifiers
  • scopus:84895027459
ISSN
0142-9612
DOI
10.1016/j.biomaterials.2013.11.078
language
English
LU publication?
no
id
d55b2906-55d5-4e04-87b3-bc296d6e41e7
date added to LUP
2017-08-15 15:04:02
date last changed
2017-10-01 05:38:35
@article{d55b2906-55d5-4e04-87b3-bc296d6e41e7,
  abstract     = {<p>Acellular scaffolds from complex whole organs such as lung are being increasingly studied for ex vivo organ generation and for in vitro studies of cell-extracellular matrix interactions. We have established effective methods for efficient de and recellularization of large animal and human lungs including techniques which allow multiple small segments (~1-3 cm<sup>3</sup>) to be excised that retain 3-dimensional lung structure. Coupled with the use of a synthetic pleural coating, cells can be selectively physiologically inoculated via preserved vascular and airway conduits. Inoculated segments can be further sliced for high throughput studies. Further, we demonstrate thermography as a powerful noninvasive technique for monitoring perfusion decellularization and for evaluating preservation of vascular and airway networks following human and porcine lung decellularization. Collectively, these techniques are a significant step forward as they allow high throughput in vitro studies from a single lung or lobe in a more biologically relevant, three-dimensional acellular scaffold.</p>},
  author       = {Wagner, Darcy E. and Bonenfant, Nicholas R. and Sokocevic, Dino and DeSarno, Michael J and Borg, Zachary D. and Parsons, Charles S. and Brooks, Elice M. and Platz, Joseph and Khalpey, Zain I. and Hoganson, David M. and Deng, Bin and Lam, Ying Wai and Oldinski, Rachael A. and Ashikaga, Takamaru and Weiss, Daniel J.},
  issn         = {0142-9612},
  keyword      = {Acellular matrix,Endothelial cell,Epithelial cell,Extracellular matrix (ECM),Human lung fibroblast,Mesenchymal stem cell},
  language     = {eng},
  number       = {9},
  pages        = {2664--2679},
  publisher    = {Elsevier},
  series       = {Biomaterials},
  title        = {Three-dimensional scaffolds of acellular human and porcine lungs for high throughput studies of lung disease and regeneration},
  url          = {http://dx.doi.org/10.1016/j.biomaterials.2013.11.078},
  volume       = {35},
  year         = {2014},
}