Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Gold and cobalt oxide nanoparticles modified poly‐propylene poly‐ethylene glycol membranes in poly (E‐caprolactone) conduits enhance nerve regeneration in the sciatic nerve of healthy rats

Hazer Rosberg, Derya Burcu LU orcid ; Hazer, Baki ; Stenberg, Lena LU orcid and Dahlin, Lars B. LU orcid (2021) In International Journal of Molecular Sciences 22(13).
Abstract

Reconstruction of nerve defects is a clinical challenge. Autologous nerve grafts as the gold standard treatment may result in an incomplete restoration of extremity function. Biosynthetic nerve conduits are studied widely, but still have limitations. Here, we reconstructed a 10 mm sciatic nerve defect in healthy rats and analyzed nerve regeneration in poly (ɛ‐caprolactone) (PCL) conduits longitudinally divided by gold (Au) and gold‐cobalt oxide (AuCoO) nanoparticles embedded in poly‐propylene poly‐ethylene glycol (PPEG) membranes (AuPPEG or AuCoOPPEG) and compared it with unmodified PPEG‐membrane and hollow PCL conduits. After 21 days, we detected significantly better axonal outgrowth, together with higher numbers of activated Schwann... (More)

Reconstruction of nerve defects is a clinical challenge. Autologous nerve grafts as the gold standard treatment may result in an incomplete restoration of extremity function. Biosynthetic nerve conduits are studied widely, but still have limitations. Here, we reconstructed a 10 mm sciatic nerve defect in healthy rats and analyzed nerve regeneration in poly (ɛ‐caprolactone) (PCL) conduits longitudinally divided by gold (Au) and gold‐cobalt oxide (AuCoO) nanoparticles embedded in poly‐propylene poly‐ethylene glycol (PPEG) membranes (AuPPEG or AuCoOPPEG) and compared it with unmodified PPEG‐membrane and hollow PCL conduits. After 21 days, we detected significantly better axonal outgrowth, together with higher numbers of activated Schwann cells (ATF3‐labelled) and higher HSP27 expression, in reconstructed sciatic nerve and in corresponding dorsal root ganglia (DRG) in the AuPPEG and AuCoOPPEG groups; whereas the number of apoptotic Schwann cells (cleaved caspase 3‐labelled) was significantly lower. Furthermore, numbers of activated and apoptotic Schwann cells in the regenerative matrix correlated with axonal outgrowth, whereas HSP27 expression in the regenerative matrix and in DRGs did not show any correlation with axonal outgrowth. We conclude that gold and cobalt‐oxide nanoparticle modified membranes in conduits improve axonal outgrowth and increase the regenerative performance of conduits after nerve reconstruction.

(Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Axonal regeneration, Cobalt oxide nanoparticle, Gold nanoparticle, Heat‐shock protein 27, Nerve conduits, Poly‐caprolactone, Poly‐ethylene glycol
in
International Journal of Molecular Sciences
volume
22
issue
13
article number
7146
publisher
MDPI AG
external identifiers
  • pmid:34281198
  • scopus:85108945380
ISSN
1661-6596
DOI
10.3390/ijms22137146
project
Nerve regeneration in healthy and diabetic rat sciatic nerve - influence by timing, a novel scaffold and the synthetic peptide PLX01
language
English
LU publication?
yes
id
d5ab8b47-0d92-46f9-8786-a8520ac900a6
date added to LUP
2021-08-18 11:39:40
date last changed
2025-08-25 13:48:30
@article{d5ab8b47-0d92-46f9-8786-a8520ac900a6,
  abstract     = {{<p>Reconstruction of nerve defects is a clinical challenge. Autologous nerve grafts as the gold standard treatment may result in an incomplete restoration of extremity function. Biosynthetic nerve conduits are studied widely, but still have limitations. Here, we reconstructed a 10 mm sciatic nerve defect in healthy rats and analyzed nerve regeneration in poly (ɛ‐caprolactone) (PCL) conduits longitudinally divided by gold (Au) and gold‐cobalt oxide (AuCoO) nanoparticles embedded in poly‐propylene poly‐ethylene glycol (PPEG) membranes (AuPPEG or AuCoOPPEG) and compared it with unmodified PPEG‐membrane and hollow PCL conduits. After 21 days, we detected significantly better axonal outgrowth, together with higher numbers of activated Schwann cells (ATF3‐labelled) and higher HSP27 expression, in reconstructed sciatic nerve and in corresponding dorsal root ganglia (DRG) in the AuPPEG and AuCoOPPEG groups; whereas the number of apoptotic Schwann cells (cleaved caspase 3‐labelled) was significantly lower. Furthermore, numbers of activated and apoptotic Schwann cells in the regenerative matrix correlated with axonal outgrowth, whereas HSP27 expression in the regenerative matrix and in DRGs did not show any correlation with axonal outgrowth. We conclude that gold and cobalt‐oxide nanoparticle modified membranes in conduits improve axonal outgrowth and increase the regenerative performance of conduits after nerve reconstruction.</p>}},
  author       = {{Hazer Rosberg, Derya Burcu and Hazer, Baki and Stenberg, Lena and Dahlin, Lars B.}},
  issn         = {{1661-6596}},
  keywords     = {{Axonal regeneration; Cobalt oxide nanoparticle; Gold nanoparticle; Heat‐shock protein 27; Nerve conduits; Poly‐caprolactone; Poly‐ethylene glycol}},
  language     = {{eng}},
  number       = {{13}},
  publisher    = {{MDPI AG}},
  series       = {{International Journal of Molecular Sciences}},
  title        = {{Gold and cobalt oxide nanoparticles modified poly‐propylene poly‐ethylene glycol membranes in poly (E‐caprolactone) conduits enhance nerve regeneration in the sciatic nerve of healthy rats}},
  url          = {{http://dx.doi.org/10.3390/ijms22137146}},
  doi          = {{10.3390/ijms22137146}},
  volume       = {{22}},
  year         = {{2021}},
}