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Characterization of neurotrophic activity in the silicone-chamber model for nerve regeneration

Danielsen, N LU and Varon, S (1995) In Journal of Reconstructive Microsurgery 11(3). p.231-235
Abstract

In the present study, the authors reevaluated the temporal course and properties of neurotrophic activities present in the fluid accumulating in the silicone-chamber model for nerve regeneration. The fluid collected from silicone chambers was tested in four different dissociated neuronal cell cultures. Furthermore, the activity of the chamber fluid was examined, using a cell blot technique. There was one major peak of neurotrophic activity and this activity peaked early, about 3 to 6 hr after nerve injury. Results also indicate that the chamber fluid contains at least two types of neurotrophic activities, namely nerve growth factor and ciliary neurotrophic factor.

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organization
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type
Contribution to journal
publication status
published
subject
keywords
Animals, Cells, Cultured, Ciliary Neurotrophic Factor, Diffusion Chambers, Culture, Female, Nerve Growth Factors, Nerve Regeneration, Nerve Tissue Proteins, Neurons, Rats, Rats, Sprague-Dawley, Sciatic Nerve, Journal Article, Research Support, Non-U.S. Gov't
in
Journal of Reconstructive Microsurgery
volume
11
issue
3
pages
5 pages
publisher
Georg Thieme Verlag
external identifiers
  • scopus:0029065435
ISSN
0743-684X
DOI
10.1055/s-2007-1006537
language
English
LU publication?
yes
id
d6540184-fd41-465f-a477-e3ea08e46da9
date added to LUP
2017-10-13 13:40:03
date last changed
2017-11-29 12:19:02
@article{d6540184-fd41-465f-a477-e3ea08e46da9,
  abstract     = {<p>In the present study, the authors reevaluated the temporal course and properties of neurotrophic activities present in the fluid accumulating in the silicone-chamber model for nerve regeneration. The fluid collected from silicone chambers was tested in four different dissociated neuronal cell cultures. Furthermore, the activity of the chamber fluid was examined, using a cell blot technique. There was one major peak of neurotrophic activity and this activity peaked early, about 3 to 6 hr after nerve injury. Results also indicate that the chamber fluid contains at least two types of neurotrophic activities, namely nerve growth factor and ciliary neurotrophic factor.</p>},
  author       = {Danielsen, N and Varon, S},
  issn         = {0743-684X},
  keyword      = {Animals,Cells, Cultured,Ciliary Neurotrophic Factor,Diffusion Chambers, Culture,Female,Nerve Growth Factors,Nerve Regeneration,Nerve Tissue Proteins,Neurons,Rats,Rats, Sprague-Dawley,Sciatic Nerve,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {3},
  pages        = {231--235},
  publisher    = {Georg Thieme Verlag},
  series       = {Journal of Reconstructive Microsurgery},
  title        = {Characterization of neurotrophic activity in the silicone-chamber model for nerve regeneration},
  url          = {http://dx.doi.org/10.1055/s-2007-1006537},
  volume       = {11},
  year         = {1995},
}