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Gastrointestinal microbiota contributes to the development of murine transfusion-related acute lung injury

Kapur, Rick LU ; Kim, Michael ; Rebetz, Johan LU orcid ; Hallström, Björn LU ; Björkman, Jonas T ; Takabe-French, Alisa ; Kim, Noel ; Liu, Jonathan ; Shanmugabhavananthan, Shanjeevan and Milosevic, Stefan , et al. (2018) In Blood Advances 2(13). p.1651-1663
Abstract

Transfusion-related acute lung injury (TRALI) is a syndrome of respiratory distress upon blood transfusion and is the leading cause of transfusion-related fatalities. Whether the gut microbiota plays any role in the development of TRALI is currently unknown. We observed that untreated barrier-free (BF) mice suffered from severe antibody-mediated acute lung injury, whereas the more sterile housed specific pathogen-free (SPF) mice and gut flora-depleted BF mice were both protected from lung injury. The prevention of TRALI in the SPF mice and gut flora-depleted BF mice was associated with decreased plasma macrophage inflammatory protein-2 levels as well as decreased pulmonary neutrophil accumulation. DNA sequencing of amplicons of the 16S... (More)

Transfusion-related acute lung injury (TRALI) is a syndrome of respiratory distress upon blood transfusion and is the leading cause of transfusion-related fatalities. Whether the gut microbiota plays any role in the development of TRALI is currently unknown. We observed that untreated barrier-free (BF) mice suffered from severe antibody-mediated acute lung injury, whereas the more sterile housed specific pathogen-free (SPF) mice and gut flora-depleted BF mice were both protected from lung injury. The prevention of TRALI in the SPF mice and gut flora-depleted BF mice was associated with decreased plasma macrophage inflammatory protein-2 levels as well as decreased pulmonary neutrophil accumulation. DNA sequencing of amplicons of the 16S ribosomal RNA gene revealed a varying gastrointestinal bacterial composition between BF and SPF mice. BF fecal matter transferred into SPF mice significantly restored TRALI susceptibility in SPF mice. These data reveal a link between the gut flora composition and the development of antibody-mediated TRALI in mice. Assessment of gut microbial composition may help in TRALI risk assessment before transfusion.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Blood Advances
volume
2
issue
13
pages
1651 - 1663
publisher
American Society of Hematology
external identifiers
  • scopus:85055233556
  • pmid:29991496
ISSN
2473-9529
DOI
10.1182/bloodadvances.2018018903
language
English
LU publication?
yes
id
d6cb0f0d-9f0d-4abd-9b45-fc05b997f481
date added to LUP
2018-08-06 16:24:36
date last changed
2022-08-17 19:42:17
@article{d6cb0f0d-9f0d-4abd-9b45-fc05b997f481,
  abstract     = {{<p>Transfusion-related acute lung injury (TRALI) is a syndrome of respiratory distress upon blood transfusion and is the leading cause of transfusion-related fatalities. Whether the gut microbiota plays any role in the development of TRALI is currently unknown. We observed that untreated barrier-free (BF) mice suffered from severe antibody-mediated acute lung injury, whereas the more sterile housed specific pathogen-free (SPF) mice and gut flora-depleted BF mice were both protected from lung injury. The prevention of TRALI in the SPF mice and gut flora-depleted BF mice was associated with decreased plasma macrophage inflammatory protein-2 levels as well as decreased pulmonary neutrophil accumulation. DNA sequencing of amplicons of the 16S ribosomal RNA gene revealed a varying gastrointestinal bacterial composition between BF and SPF mice. BF fecal matter transferred into SPF mice significantly restored TRALI susceptibility in SPF mice. These data reveal a link between the gut flora composition and the development of antibody-mediated TRALI in mice. Assessment of gut microbial composition may help in TRALI risk assessment before transfusion.</p>}},
  author       = {{Kapur, Rick and Kim, Michael and Rebetz, Johan and Hallström, Björn and Björkman, Jonas T and Takabe-French, Alisa and Kim, Noel and Liu, Jonathan and Shanmugabhavananthan, Shanjeevan and Milosevic, Stefan and McVey, Mark J and Speck, Edwin R and Semple, John W}},
  issn         = {{2473-9529}},
  language     = {{eng}},
  month        = {{07}},
  number       = {{13}},
  pages        = {{1651--1663}},
  publisher    = {{American Society of Hematology}},
  series       = {{Blood Advances}},
  title        = {{Gastrointestinal microbiota contributes to the development of murine transfusion-related acute lung injury}},
  url          = {{http://dx.doi.org/10.1182/bloodadvances.2018018903}},
  doi          = {{10.1182/bloodadvances.2018018903}},
  volume       = {{2}},
  year         = {{2018}},
}