Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Label-free fast 3D coherent imaging reveals pancreatic islet micro-vascularization and dynamic blood flow

Berclaz, Corinne ; Szlag, Daniel ; Nguyen, David ; Extermann, Jérôme ; Bouwens, Arno ; Marchand, Paul J. ; Nilsson, Julia LU ; Schmidt-Christensen, Anja LU orcid ; Holmberg, Dan LU and Grapin-Botton, Anne , et al. (2016) In Biomedical Optics Express 7(11). p.4569-4580
Abstract

In diabetes, pancreatic ß-cells play a key role. These cells are clustered within structures called islets of Langerhans inside the pancreas and produce insulin, which is directly secreted into the blood stream. The dense vascularization of islets of Langerhans is critical for maintaining a proper regulation of blood glucose homeostasis and is known to be affected from the early stage of diabetes. The deep localization of these islets inside the pancreas in the abdominal cavity renders their in vivo visualization a challenging task. A fast label-free imaging method with high spatial resolution is required to study the vascular network of islets of Langerhans. Based on these requirements, we developed a label-free and three-dimensional... (More)

In diabetes, pancreatic ß-cells play a key role. These cells are clustered within structures called islets of Langerhans inside the pancreas and produce insulin, which is directly secreted into the blood stream. The dense vascularization of islets of Langerhans is critical for maintaining a proper regulation of blood glucose homeostasis and is known to be affected from the early stage of diabetes. The deep localization of these islets inside the pancreas in the abdominal cavity renders their in vivo visualization a challenging task. A fast label-free imaging method with high spatial resolution is required to study the vascular network of islets of Langerhans. Based on these requirements, we developed a label-free and three-dimensional imaging method for observing islets of Langerhans using extended-focus Fourier domain Optical Coherence Microscopy (xfOCM). In addition to structural imaging, this system provides threedimensional vascular network imaging and dynamic blood flow information within islets of Langerhans. We propose our method to deepen the understanding of the interconnection between diabetes and the evolution of the islet vascular network.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and , et al. (More)
; ; ; ; ; ; ; ; ; and (Less)
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Biology, Flow diagnostics, Optical coherence tomography, Three-dimensional image acquisition
in
Biomedical Optics Express
volume
7
issue
11
article number
#273588
pages
12 pages
publisher
Optical Society of America
external identifiers
  • pmid:27895996
  • wos:000387557600018
  • scopus:84994545324
ISSN
2156-7085
DOI
10.1364/BOE.7.004569
project
inflammatory events leading to autoimmune Diabetes
3D imaging of inflammation and autoimmunity
language
English
LU publication?
yes
id
d751fdd0-f68e-4094-b032-f9d5ba1bd706
date added to LUP
2016-12-05 13:37:57
date last changed
2024-10-05 07:21:13
@article{d751fdd0-f68e-4094-b032-f9d5ba1bd706,
  abstract     = {{<p>In diabetes, pancreatic ß-cells play a key role. These cells are clustered within structures called islets of Langerhans inside the pancreas and produce insulin, which is directly secreted into the blood stream. The dense vascularization of islets of Langerhans is critical for maintaining a proper regulation of blood glucose homeostasis and is known to be affected from the early stage of diabetes. The deep localization of these islets inside the pancreas in the abdominal cavity renders their in vivo visualization a challenging task. A fast label-free imaging method with high spatial resolution is required to study the vascular network of islets of Langerhans. Based on these requirements, we developed a label-free and three-dimensional imaging method for observing islets of Langerhans using extended-focus Fourier domain Optical Coherence Microscopy (xfOCM). In addition to structural imaging, this system provides threedimensional vascular network imaging and dynamic blood flow information within islets of Langerhans. We propose our method to deepen the understanding of the interconnection between diabetes and the evolution of the islet vascular network.</p>}},
  author       = {{Berclaz, Corinne and Szlag, Daniel and Nguyen, David and Extermann, Jérôme and Bouwens, Arno and Marchand, Paul J. and Nilsson, Julia and Schmidt-Christensen, Anja and Holmberg, Dan and Grapin-Botton, Anne and Lasser, Theo}},
  issn         = {{2156-7085}},
  keywords     = {{Biology; Flow diagnostics; Optical coherence tomography; Three-dimensional image acquisition}},
  language     = {{eng}},
  month        = {{11}},
  number       = {{11}},
  pages        = {{4569--4580}},
  publisher    = {{Optical Society of America}},
  series       = {{Biomedical Optics Express}},
  title        = {{Label-free fast 3D coherent imaging reveals pancreatic islet micro-vascularization and dynamic blood flow}},
  url          = {{http://dx.doi.org/10.1364/BOE.7.004569}},
  doi          = {{10.1364/BOE.7.004569}},
  volume       = {{7}},
  year         = {{2016}},
}