Fluorescence microscopy of nanochannel-confined DNA
Westerlund, Fredrik ; Persson, Fredrik ; Fritzsche, Joachim ; Beech, Jason P. LU and Tegenfeldt, Jonas O. LU
(2018)
In Methods in Molecular Biology
1665.
p.173-198
- Abstract
Stretching of DNA in nanoscale confinement allows for several important studies. The genetic contents of the DNA can be visualized on the single DNA molecule level and both the polymer physics of confined DNA and also DNA/protein and other DNA/DNA-binding molecule interactions can be explored. This chapter describes the basic steps to fabricate the nanostructures, perform the experiments and analyze the data.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/d786dea2-a87b-4bef-8da6-65a3ac122f97
- author
- Westerlund, Fredrik
; Persson, Fredrik
; Fritzsche, Joachim
; Beech, Jason P.
LU
and Tegenfeldt, Jonas O.
LU
- organization
- publishing date
- 2018
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- DNA, Fluorescence, Nanochannels, Single molecule
- host publication
- Methods in Molecular Biology
- series title
- Methods in Molecular Biology
- volume
- 1665
- pages
- 26 pages
- publisher
- Humana Press
- external identifiers
-
- pmid:28940070
- scopus:85030026157
- ISSN
- 10643745
- DOI
- 10.1007/978-1-4939-7271-5_10
- language
- English
- LU publication?
- yes
- id
- d786dea2-a87b-4bef-8da6-65a3ac122f97
- date added to LUP
- 2017-10-10 09:23:25
- date last changed
- 2024-01-29 03:53:50
@inbook{d786dea2-a87b-4bef-8da6-65a3ac122f97,
abstract = {{<p>Stretching of DNA in nanoscale confinement allows for several important studies. The genetic contents of the DNA can be visualized on the single DNA molecule level and both the polymer physics of confined DNA and also DNA/protein and other DNA/DNA-binding molecule interactions can be explored. This chapter describes the basic steps to fabricate the nanostructures, perform the experiments and analyze the data.</p>}},
author = {{Westerlund, Fredrik and Persson, Fredrik and Fritzsche, Joachim and Beech, Jason P. and Tegenfeldt, Jonas O.}},
booktitle = {{Methods in Molecular Biology}},
issn = {{10643745}},
keywords = {{DNA; Fluorescence; Nanochannels; Single molecule}},
language = {{eng}},
pages = {{173--198}},
publisher = {{Humana Press}},
series = {{Methods in Molecular Biology}},
title = {{Fluorescence microscopy of nanochannel-confined DNA}},
url = {{http://dx.doi.org/10.1007/978-1-4939-7271-5_10}},
doi = {{10.1007/978-1-4939-7271-5_10}},
volume = {{1665}},
year = {{2018}},
}