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Fluorescence microscopy of nanochannel-confined DNA

Westerlund, Fredrik; Persson, Fredrik; Fritzsche, Joachim; Beech, Jason P. LU and Tegenfeldt, Jonas O. LU (2018) In Methods in Molecular Biology 1665. p.173-198
Abstract

Stretching of DNA in nanoscale confinement allows for several important studies. The genetic contents of the DNA can be visualized on the single DNA molecule level and both the polymer physics of confined DNA and also DNA/protein and other DNA/DNA-binding molecule interactions can be explored. This chapter describes the basic steps to fabricate the nanostructures, perform the experiments and analyze the data.

Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
DNA, Fluorescence, Nanochannels, Single molecule
in
Methods in Molecular Biology
volume
1665
pages
26 pages
publisher
Humana Press
external identifiers
  • scopus:85030026157
ISSN
10643745
DOI
10.1007/978-1-4939-7271-5_10
language
English
LU publication?
yes
id
d786dea2-a87b-4bef-8da6-65a3ac122f97
date added to LUP
2017-10-10 09:23:25
date last changed
2018-01-07 12:21:43
@inbook{d786dea2-a87b-4bef-8da6-65a3ac122f97,
  abstract     = {<p>Stretching of DNA in nanoscale confinement allows for several important studies. The genetic contents of the DNA can be visualized on the single DNA molecule level and both the polymer physics of confined DNA and also DNA/protein and other DNA/DNA-binding molecule interactions can be explored. This chapter describes the basic steps to fabricate the nanostructures, perform the experiments and analyze the data.</p>},
  author       = {Westerlund, Fredrik and Persson, Fredrik and Fritzsche, Joachim and Beech, Jason P. and Tegenfeldt, Jonas O.},
  issn         = {10643745},
  keyword      = {DNA,Fluorescence,Nanochannels,Single molecule},
  language     = {eng},
  pages        = {173--198},
  publisher    = {Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {Fluorescence microscopy of nanochannel-confined DNA},
  url          = {http://dx.doi.org/10.1007/978-1-4939-7271-5_10},
  volume       = {1665},
  year         = {2018},
}