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Microvascular effects of chondroitinase ABC and chymopapain. An in vivo experimental study on hamsters and rabbits

Olmarker, K; Danielsen, N LU ; Nannmark, U; Sennerby, L and Rydevik, B (1990) In Clinical Orthopaedics and Related Research p.9-274
Abstract

Immediate and long-term microvascular effects of chondroitinase ABC, 200 unit/ml, were analyzed in ten hamsters. The immediate effects on the microcirculation were studied by vital microscopy following local injection in the cheek pouch. There were no detectable effects on the microvascular blood flow during the 60 minutes of observation for chondroitinase ABC or the control. A therapeutic concentration (2000 pKat/ml) of chymopapain stopped the microcirculation in the injected area immediately, with numerous microbleedings at the border zone. Long-term effects were studied after subcutaneous injections in the ears of six rabbits. Chondroitinase ABC and the control did not cause any macroscopic or microangiographic effects. However,... (More)

Immediate and long-term microvascular effects of chondroitinase ABC, 200 unit/ml, were analyzed in ten hamsters. The immediate effects on the microcirculation were studied by vital microscopy following local injection in the cheek pouch. There were no detectable effects on the microvascular blood flow during the 60 minutes of observation for chondroitinase ABC or the control. A therapeutic concentration (2000 pKat/ml) of chymopapain stopped the microcirculation in the injected area immediately, with numerous microbleedings at the border zone. Long-term effects were studied after subcutaneous injections in the ears of six rabbits. Chondroitinase ABC and the control did not cause any macroscopic or microangiographic effects. However, light microscopy showed a moderate inflammatory reaction in the subcutaneous layer for both chondroitinase ABC and the control. Chymopapain induced severe effects on the cartilage and surrounding tissues. Microangiography revealed a vessel-free zone at the injection site. Since 200 units/ml of chondroitinase ABC is four to eight times higher than the concentration that might be used for chemonucleolysis, i.e., dissolution of intervertebral discs by local enzyme injection, the present investigation suggests a wide margin of safety regarding the potential effects on blood vessels in tissues surrounding the disc.

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author
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Contribution to journal
publication status
published
keywords
Animals, Cartilage, Cheek, Chondroitin Lyases, Chondroitinases and Chondroitin Lyases, Chymopapain, Cricetinae, Mesocricetus, Microcirculation, Microscopy, Mouth Mucosa, Rabbits, Journal Article, Research Support, Non-U.S. Gov't
in
Clinical Orthopaedics and Related Research
issue
257
pages
6 pages
publisher
Lippincott Williams & Wilkins
external identifiers
  • scopus:0025163510
ISSN
0009-921X
language
English
LU publication?
no
id
d808ca95-9c7e-4268-bd52-0908aa0f04c1
date added to LUP
2017-10-13 14:12:59
date last changed
2017-11-07 16:14:40
@article{d808ca95-9c7e-4268-bd52-0908aa0f04c1,
  abstract     = {<p>Immediate and long-term microvascular effects of chondroitinase ABC, 200 unit/ml, were analyzed in ten hamsters. The immediate effects on the microcirculation were studied by vital microscopy following local injection in the cheek pouch. There were no detectable effects on the microvascular blood flow during the 60 minutes of observation for chondroitinase ABC or the control. A therapeutic concentration (2000 pKat/ml) of chymopapain stopped the microcirculation in the injected area immediately, with numerous microbleedings at the border zone. Long-term effects were studied after subcutaneous injections in the ears of six rabbits. Chondroitinase ABC and the control did not cause any macroscopic or microangiographic effects. However, light microscopy showed a moderate inflammatory reaction in the subcutaneous layer for both chondroitinase ABC and the control. Chymopapain induced severe effects on the cartilage and surrounding tissues. Microangiography revealed a vessel-free zone at the injection site. Since 200 units/ml of chondroitinase ABC is four to eight times higher than the concentration that might be used for chemonucleolysis, i.e., dissolution of intervertebral discs by local enzyme injection, the present investigation suggests a wide margin of safety regarding the potential effects on blood vessels in tissues surrounding the disc.</p>},
  author       = {Olmarker, K and Danielsen, N and Nannmark, U and Sennerby, L and Rydevik, B},
  issn         = {0009-921X},
  keyword      = {Animals,Cartilage,Cheek,Chondroitin Lyases,Chondroitinases and Chondroitin Lyases,Chymopapain,Cricetinae,Mesocricetus,Microcirculation,Microscopy,Mouth Mucosa,Rabbits,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {257},
  pages        = {9--274},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {Clinical Orthopaedics and Related Research},
  title        = {Microvascular effects of chondroitinase ABC and chymopapain. An in vivo experimental study on hamsters and rabbits},
  year         = {1990},
}