Anaerobic fixed-target serial crystallography using sandwiched silicon nitride membranes
(2023) In Acta Crystallographica Section D: Structural Biology 79(Pt 11). p.1018-1025- Abstract
In recent years, the emergence of serial crystallography, initially pioneered at X-ray free-electron lasers (XFELs), has sparked a growing interest in collecting macromolecular crystallographic data at room temperature. Various fixed-target serial crystallography techniques have been developed, ranging from commercially available chips to in-house designs implemented at different synchrotron facilities. Nevertheless, there is currently no commercially available chip (known to the authors) specifically designed for the direct handling of oxygen-sensitive samples. This study presents a methodology employing silicon nitride chips arranged in a 'sandwich' configuration, enabling reliable room-temperature data collection from... (More)
In recent years, the emergence of serial crystallography, initially pioneered at X-ray free-electron lasers (XFELs), has sparked a growing interest in collecting macromolecular crystallographic data at room temperature. Various fixed-target serial crystallography techniques have been developed, ranging from commercially available chips to in-house designs implemented at different synchrotron facilities. Nevertheless, there is currently no commercially available chip (known to the authors) specifically designed for the direct handling of oxygen-sensitive samples. This study presents a methodology employing silicon nitride chips arranged in a 'sandwich' configuration, enabling reliable room-temperature data collection from oxygen-sensitive samples. The method involves the utilization of a custom-made 3D-printed assembling tool and a MX sample holder. To validate the effectiveness of the proposed method, deoxyhemoglobin and methemoglobin samples were investigated using the BioMAX X-ray macromolecular crystallography beamline, the Balder X-ray absorption spectroscopy beamline and UV-Vis absorption spectroscopy.
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- author
- Bjelčić, Monika LU ; Sigfridsson Clauss, Kajsa G.V. LU ; Aurelius, Oskar LU ; Milas, Mirko LU ; Nan, Jie LU and Ursby, Thomas LU
- organization
- publishing date
- 2023
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- anaerobic data collection, fixed-target serial crystallography, hemoglobin, sample supports, sandwiched silicon nitride membranes
- in
- Acta Crystallographica Section D: Structural Biology
- volume
- 79
- issue
- Pt 11
- pages
- 8 pages
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:37860963
- scopus:85175584969
- ISSN
- 2059-7983
- DOI
- 10.1107/S205979832300880X
- language
- English
- LU publication?
- yes
- id
- d8adf558-0d05-48da-8be6-5bf16c96c27d
- date added to LUP
- 2023-11-24 13:53:13
- date last changed
- 2024-04-21 16:44:52
@article{d8adf558-0d05-48da-8be6-5bf16c96c27d,
abstract = {{<p>In recent years, the emergence of serial crystallography, initially pioneered at X-ray free-electron lasers (XFELs), has sparked a growing interest in collecting macromolecular crystallographic data at room temperature. Various fixed-target serial crystallography techniques have been developed, ranging from commercially available chips to in-house designs implemented at different synchrotron facilities. Nevertheless, there is currently no commercially available chip (known to the authors) specifically designed for the direct handling of oxygen-sensitive samples. This study presents a methodology employing silicon nitride chips arranged in a 'sandwich' configuration, enabling reliable room-temperature data collection from oxygen-sensitive samples. The method involves the utilization of a custom-made 3D-printed assembling tool and a MX sample holder. To validate the effectiveness of the proposed method, deoxyhemoglobin and methemoglobin samples were investigated using the BioMAX X-ray macromolecular crystallography beamline, the Balder X-ray absorption spectroscopy beamline and UV-Vis absorption spectroscopy.</p>}},
author = {{Bjelčić, Monika and Sigfridsson Clauss, Kajsa G.V. and Aurelius, Oskar and Milas, Mirko and Nan, Jie and Ursby, Thomas}},
issn = {{2059-7983}},
keywords = {{anaerobic data collection; fixed-target serial crystallography; hemoglobin; sample supports; sandwiched silicon nitride membranes}},
language = {{eng}},
number = {{Pt 11}},
pages = {{1018--1025}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Acta Crystallographica Section D: Structural Biology}},
title = {{Anaerobic fixed-target serial crystallography using sandwiched silicon nitride membranes}},
url = {{http://dx.doi.org/10.1107/S205979832300880X}},
doi = {{10.1107/S205979832300880X}},
volume = {{79}},
year = {{2023}},
}