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Bisphosphonate ligand mediated ultrasensitive capacitive protein sensor : complementary match of supramolecular and dynamic chemistry

Ertürk, Gizem LU ; Akhoundian, Maedeh; Lueg-Althoff, Kyra; Shinde, Sudhirkumar; Yeung, Sing Yee; Hedström, Martin LU ; Schrader, Thomas; Mattiasson, Bo LU and Sellergren, Börje (2018) In New Journal of Chemistry 43(2). p.847-852
Abstract

Modern healthcare demands rapid and accurate detection of proteins/enzymes at the ultratrace level. Herein we present a molecularly imprinted capacitive sensor for trypsin, developed by microcontact imprinting. High affinity and selectivity was achieved by doping the prepolymerization mixture with a stoichiometric amount of methacrylamide-based bisphosphonate (BP) monomer. Taking advantage of the specific interaction between bisphosphonate binding monomers and lysine/arginine residues on the surface of trypsin, we have constructed a powerful polymeric sensor. The BP based sensor has the ability to recognize trypsin over other arginine-rich proteins, even in high ionic strength buffers with a sub-picomolar detection limit (pM). We... (More)

Modern healthcare demands rapid and accurate detection of proteins/enzymes at the ultratrace level. Herein we present a molecularly imprinted capacitive sensor for trypsin, developed by microcontact imprinting. High affinity and selectivity was achieved by doping the prepolymerization mixture with a stoichiometric amount of methacrylamide-based bisphosphonate (BP) monomer. Taking advantage of the specific interaction between bisphosphonate binding monomers and lysine/arginine residues on the surface of trypsin, we have constructed a powerful polymeric sensor. The BP based sensor has the ability to recognize trypsin over other arginine-rich proteins, even in high ionic strength buffers with a sub-picomolar detection limit (pM). We believe that the combination of supramolecular chemistry, molecular imprinting and advanced instrumentation has a potential for future drug development and diagnostics that extends beyond biomolecular recognition.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
New Journal of Chemistry
volume
43
issue
2
pages
6 pages
publisher
Royal Society of Chemistry
external identifiers
  • scopus:85059570875
ISSN
1144-0546
DOI
10.1039/c8nj05238g
language
English
LU publication?
yes
id
d92e068e-f783-4d9c-8656-768604783cb9
date added to LUP
2019-01-18 14:14:05
date last changed
2019-02-20 11:43:34
@article{d92e068e-f783-4d9c-8656-768604783cb9,
  abstract     = {<p>Modern healthcare demands rapid and accurate detection of proteins/enzymes at the ultratrace level. Herein we present a molecularly imprinted capacitive sensor for trypsin, developed by microcontact imprinting. High affinity and selectivity was achieved by doping the prepolymerization mixture with a stoichiometric amount of methacrylamide-based bisphosphonate (BP) monomer. Taking advantage of the specific interaction between bisphosphonate binding monomers and lysine/arginine residues on the surface of trypsin, we have constructed a powerful polymeric sensor. The BP based sensor has the ability to recognize trypsin over other arginine-rich proteins, even in high ionic strength buffers with a sub-picomolar detection limit (pM). We believe that the combination of supramolecular chemistry, molecular imprinting and advanced instrumentation has a potential for future drug development and diagnostics that extends beyond biomolecular recognition.</p>},
  author       = {Ertürk, Gizem and Akhoundian, Maedeh and Lueg-Althoff, Kyra and Shinde, Sudhirkumar and Yeung, Sing Yee and Hedström, Martin and Schrader, Thomas and Mattiasson, Bo and Sellergren, Börje},
  issn         = {1144-0546},
  language     = {eng},
  month        = {12},
  number       = {2},
  pages        = {847--852},
  publisher    = {Royal Society of Chemistry},
  series       = {New Journal of Chemistry},
  title        = {Bisphosphonate ligand mediated ultrasensitive capacitive protein sensor : complementary match of supramolecular and dynamic chemistry},
  url          = {http://dx.doi.org/10.1039/c8nj05238g},
  volume       = {43},
  year         = {2018},
}