Fluorescence polarisation for immunoreagent characterisation
Önnerfjord, P. LU
; Eremin, S.
; Emnéus, J.
LU
and Marko-Varga, G.
LU
(1998)
In Journal of Immunological Methods
213(1).
p.31-39
- Abstract
Antibodies were characterised using fluorescence polarisation, a homogeneous assay technique in which all reagents are in solution. Kinetic studies on the association and dissociation of the immunocomplex were performed. A competitive assay was used and the sensitivities, operational linearities, as well as the specificities of the immunoassays were experimentally determined for various antibody preparations with specificity for triazines. Detection limits for atrazine in water samples were determined to be within the range of 0.08-0.4 ng ml-1 using a 5-min incubation time and a 0.5-ml sample volume.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/d953be59-1b7c-42cf-9371-21973892e1de
- author
- Önnerfjord, P.
LU
; Eremin, S.
; Emnéus, J.
LU
and Marko-Varga, G.
LU
- organization
- publishing date
- 1998-04-01
- type
- Contribution to journal
- publication status
- published
- keywords
- Antibody characterisation, Atrazine, Fluorescence polarisation, Homogenous immunoassay, Triazines
- in
- Journal of Immunological Methods
- volume
- 213
- issue
- 1
- pages
- 9 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:9671123
- scopus:0031845984
- ISSN
- 0022-1759
- DOI
- 10.1016/S0022-1759(98)00019-2
- language
- English
- LU publication?
- yes
- id
- d953be59-1b7c-42cf-9371-21973892e1de
- date added to LUP
- 2016-10-14 11:37:38
- date last changed
- 2025-01-12 13:14:50
@article{d953be59-1b7c-42cf-9371-21973892e1de,
abstract = {{<p>Antibodies were characterised using fluorescence polarisation, a homogeneous assay technique in which all reagents are in solution. Kinetic studies on the association and dissociation of the immunocomplex were performed. A competitive assay was used and the sensitivities, operational linearities, as well as the specificities of the immunoassays were experimentally determined for various antibody preparations with specificity for triazines. Detection limits for atrazine in water samples were determined to be within the range of 0.08-0.4 ng ml<sup>-1</sup> using a 5-min incubation time and a 0.5-ml sample volume.</p>}},
author = {{Önnerfjord, P. and Eremin, S. and Emnéus, J. and Marko-Varga, G.}},
issn = {{0022-1759}},
keywords = {{Antibody characterisation; Atrazine; Fluorescence polarisation; Homogenous immunoassay; Triazines}},
language = {{eng}},
month = {{04}},
number = {{1}},
pages = {{31--39}},
publisher = {{Elsevier}},
series = {{Journal of Immunological Methods}},
title = {{Fluorescence polarisation for immunoreagent characterisation}},
url = {{http://dx.doi.org/10.1016/S0022-1759(98)00019-2}},
doi = {{10.1016/S0022-1759(98)00019-2}},
volume = {{213}},
year = {{1998}},
}