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Mixtures of mucin and oppositely charged surfactant aggregates with varying charge density. Phase behavior, association, and dynamics

Lafitte, Géraldine LU ; Thuresson, Krister LU and Söderman, Olle LU (2005) In Langmuir 21(16). p.7097-7104
Abstract
The nonionic surfactant Tween80 is a commonly used excipient in drug formulations containing an active substance with low aqueous solubility. Model drug vehicles with varying charge density were obtained by mixing Tween80 (PS-80) with the cationic surfactant Tetradecyltrimethylammonium chloride (TTAC), thus forming mixed micelles. The micelles were mixed with the negatively charged polyelectrolyte mucin, which is a component in the protective mucus layer covering epithelial cell linings. Depending on the composition of the mixture, complex-formation could be followed by precipitation. Using X-ray diffraction, it was found that the precipitate contained a lamellar phase with properties sensitive to the proportion of PS-80. Higher amounts of... (More)
The nonionic surfactant Tween80 is a commonly used excipient in drug formulations containing an active substance with low aqueous solubility. Model drug vehicles with varying charge density were obtained by mixing Tween80 (PS-80) with the cationic surfactant Tetradecyltrimethylammonium chloride (TTAC), thus forming mixed micelles. The micelles were mixed with the negatively charged polyelectrolyte mucin, which is a component in the protective mucus layer covering epithelial cell linings. Depending on the composition of the mixture, complex-formation could be followed by precipitation. Using X-ray diffraction, it was found that the precipitate contained a lamellar phase with properties sensitive to the proportion of PS-80. Higher amounts of PS-80 were found to oppose phase separation. Further analysis in the one-phase region, or alternatively of the supernatant of two-phase samples, by H-1 NMR, HPLC, and diffusion measurements with PGSE-NMR led to the conclusions that at low proportion of PS-80 aggregates composed of mixed (PS-80 and TTAC) micelles and mucin were formed, whereas increased concentrations of PS-80 favored the dissolution of the precipitate and limited the interactions between mixed micelles and the polymer. (Less)
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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
21
issue
16
pages
7097 - 7104
publisher
The American Chemical Society (ACS)
external identifiers
  • pmid:16042429
  • wos:000230839600008
  • scopus:23844559323
ISSN
0743-7463
DOI
10.1021/la0472807
language
English
LU publication?
yes
id
d9608baf-a5d1-49b5-8ada-e27a00b92f88 (old id 157440)
date added to LUP
2016-04-01 12:31:48
date last changed
2025-10-14 12:19:31
@article{d9608baf-a5d1-49b5-8ada-e27a00b92f88,
  abstract     = {{The nonionic surfactant Tween80 is a commonly used excipient in drug formulations containing an active substance with low aqueous solubility. Model drug vehicles with varying charge density were obtained by mixing Tween80 (PS-80) with the cationic surfactant Tetradecyltrimethylammonium chloride (TTAC), thus forming mixed micelles. The micelles were mixed with the negatively charged polyelectrolyte mucin, which is a component in the protective mucus layer covering epithelial cell linings. Depending on the composition of the mixture, complex-formation could be followed by precipitation. Using X-ray diffraction, it was found that the precipitate contained a lamellar phase with properties sensitive to the proportion of PS-80. Higher amounts of PS-80 were found to oppose phase separation. Further analysis in the one-phase region, or alternatively of the supernatant of two-phase samples, by H-1 NMR, HPLC, and diffusion measurements with PGSE-NMR led to the conclusions that at low proportion of PS-80 aggregates composed of mixed (PS-80 and TTAC) micelles and mucin were formed, whereas increased concentrations of PS-80 favored the dissolution of the precipitate and limited the interactions between mixed micelles and the polymer.}},
  author       = {{Lafitte, Géraldine and Thuresson, Krister and Söderman, Olle}},
  issn         = {{0743-7463}},
  language     = {{eng}},
  number       = {{16}},
  pages        = {{7097--7104}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Langmuir}},
  title        = {{Mixtures of mucin and oppositely charged surfactant aggregates with varying charge density. Phase behavior, association, and dynamics}},
  url          = {{http://dx.doi.org/10.1021/la0472807}},
  doi          = {{10.1021/la0472807}},
  volume       = {{21}},
  year         = {{2005}},
}