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Mast cell tryptase enhances wound healing by promoting migration in human bronchial epithelial cells

Mogren, Sofia LU ; Berlin, Frida LU ; Ramu, Sangeetha LU ; Sverrild, Asger ; Porsbjerg, Celeste ; Uller, Lena LU and Andersson, Cecilia LU (2021) In Cell Adhesion and Migration 15(1). p.202-214
Abstract
Epithelial damage and increase of intraepithelial mast cells (MC) are characteristics of asthma. The role of MC mediator tryptase and the protease-activated receptor-2 (PAR2) on epithelial wound healing is not fully investigated. Stimulation of bronchial epithelial cells (BECs) with tryptase promoted gap closure, migration and cellular speed compared to controls. Stimulated BECs had higher expression of migration marker CD151 compared to controls. Proliferation marker KI67 was upregulated in tryptase-stimulated BECs compared to controls. Treatment with PAR2 antagonist I-191 reduced gap closure, migration and cell speed compared to BECs stimulated with tryptase. We found that tryptase enhances epithelial wound healing by increased migration... (More)
Epithelial damage and increase of intraepithelial mast cells (MC) are characteristics of asthma. The role of MC mediator tryptase and the protease-activated receptor-2 (PAR2) on epithelial wound healing is not fully investigated. Stimulation of bronchial epithelial cells (BECs) with tryptase promoted gap closure, migration and cellular speed compared to controls. Stimulated BECs had higher expression of migration marker CD151 compared to controls. Proliferation marker KI67 was upregulated in tryptase-stimulated BECs compared to controls. Treatment with PAR2 antagonist I-191 reduced gap closure, migration and cell speed compared to BECs stimulated with tryptase. We found that tryptase enhances epithelial wound healing by increased migration and proliferation, which is in part regulated via PAR2. Our data suggest that tryptase might be beneficial in tissue repair under baseline conditions. However, in a pathological context such as asthma with increased numbers of activated MCs, it might lead to epithelial remodeling and loss of function. (Less)
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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Cell Adhesion and Migration
volume
15
issue
1
pages
202 - 214
publisher
Landes Bioscience
external identifiers
  • scopus:85111119719
  • pmid:34308764
ISSN
1933-6918
DOI
10.1080/19336918.2021.1950594
language
English
LU publication?
yes
id
d97ee21c-3017-417a-85cf-17ac45dda164
date added to LUP
2021-09-17 14:55:52
date last changed
2023-04-02 17:36:22
@article{d97ee21c-3017-417a-85cf-17ac45dda164,
  abstract     = {{Epithelial damage and increase of intraepithelial mast cells (MC) are characteristics of asthma. The role of MC mediator tryptase and the protease-activated receptor-2 (PAR2) on epithelial wound healing is not fully investigated. Stimulation of bronchial epithelial cells (BECs) with tryptase promoted gap closure, migration and cellular speed compared to controls. Stimulated BECs had higher expression of migration marker CD151 compared to controls. Proliferation marker KI67 was upregulated in tryptase-stimulated BECs compared to controls. Treatment with PAR2 antagonist I-191 reduced gap closure, migration and cell speed compared to BECs stimulated with tryptase. We found that tryptase enhances epithelial wound healing by increased migration and proliferation, which is in part regulated via PAR2. Our data suggest that tryptase might be beneficial in tissue repair under baseline conditions. However, in a pathological context such as asthma with increased numbers of activated MCs, it might lead to epithelial remodeling and loss of function.}},
  author       = {{Mogren, Sofia and Berlin, Frida and Ramu, Sangeetha and Sverrild, Asger and Porsbjerg, Celeste and Uller, Lena and Andersson, Cecilia}},
  issn         = {{1933-6918}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{202--214}},
  publisher    = {{Landes Bioscience}},
  series       = {{Cell Adhesion and Migration}},
  title        = {{Mast cell tryptase enhances wound healing by promoting migration in human bronchial epithelial cells}},
  url          = {{http://dx.doi.org/10.1080/19336918.2021.1950594}},
  doi          = {{10.1080/19336918.2021.1950594}},
  volume       = {{15}},
  year         = {{2021}},
}