Mast cell tryptase enhances wound healing by promoting migration in human bronchial epithelial cells
(2021) In Cell Adhesion and Migration 15(1). p.202-214- Abstract
- Epithelial damage and increase of intraepithelial mast cells (MC) are characteristics of asthma. The role of MC mediator tryptase and the protease-activated receptor-2 (PAR2) on epithelial wound healing is not fully investigated. Stimulation of bronchial epithelial cells (BECs) with tryptase promoted gap closure, migration and cellular speed compared to controls. Stimulated BECs had higher expression of migration marker CD151 compared to controls. Proliferation marker KI67 was upregulated in tryptase-stimulated BECs compared to controls. Treatment with PAR2 antagonist I-191 reduced gap closure, migration and cell speed compared to BECs stimulated with tryptase. We found that tryptase enhances epithelial wound healing by increased migration... (More)
- Epithelial damage and increase of intraepithelial mast cells (MC) are characteristics of asthma. The role of MC mediator tryptase and the protease-activated receptor-2 (PAR2) on epithelial wound healing is not fully investigated. Stimulation of bronchial epithelial cells (BECs) with tryptase promoted gap closure, migration and cellular speed compared to controls. Stimulated BECs had higher expression of migration marker CD151 compared to controls. Proliferation marker KI67 was upregulated in tryptase-stimulated BECs compared to controls. Treatment with PAR2 antagonist I-191 reduced gap closure, migration and cell speed compared to BECs stimulated with tryptase. We found that tryptase enhances epithelial wound healing by increased migration and proliferation, which is in part regulated via PAR2. Our data suggest that tryptase might be beneficial in tissue repair under baseline conditions. However, in a pathological context such as asthma with increased numbers of activated MCs, it might lead to epithelial remodeling and loss of function. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/d97ee21c-3017-417a-85cf-17ac45dda164
- author
- Mogren, Sofia LU ; Berlin, Frida LU ; Ramu, Sangeetha LU ; Sverrild, Asger ; Porsbjerg, Celeste ; Uller, Lena LU and Andersson, Cecilia LU
- organization
- publishing date
- 2021
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Cell Adhesion and Migration
- volume
- 15
- issue
- 1
- pages
- 202 - 214
- publisher
- Landes Bioscience
- external identifiers
-
- scopus:85111119719
- pmid:34308764
- ISSN
- 1933-6918
- DOI
- 10.1080/19336918.2021.1950594
- language
- English
- LU publication?
- yes
- id
- d97ee21c-3017-417a-85cf-17ac45dda164
- date added to LUP
- 2021-09-17 14:55:52
- date last changed
- 2024-09-22 01:27:26
@article{d97ee21c-3017-417a-85cf-17ac45dda164, abstract = {{Epithelial damage and increase of intraepithelial mast cells (MC) are characteristics of asthma. The role of MC mediator tryptase and the protease-activated receptor-2 (PAR2) on epithelial wound healing is not fully investigated. Stimulation of bronchial epithelial cells (BECs) with tryptase promoted gap closure, migration and cellular speed compared to controls. Stimulated BECs had higher expression of migration marker CD151 compared to controls. Proliferation marker KI67 was upregulated in tryptase-stimulated BECs compared to controls. Treatment with PAR2 antagonist I-191 reduced gap closure, migration and cell speed compared to BECs stimulated with tryptase. We found that tryptase enhances epithelial wound healing by increased migration and proliferation, which is in part regulated via PAR2. Our data suggest that tryptase might be beneficial in tissue repair under baseline conditions. However, in a pathological context such as asthma with increased numbers of activated MCs, it might lead to epithelial remodeling and loss of function.}}, author = {{Mogren, Sofia and Berlin, Frida and Ramu, Sangeetha and Sverrild, Asger and Porsbjerg, Celeste and Uller, Lena and Andersson, Cecilia}}, issn = {{1933-6918}}, language = {{eng}}, number = {{1}}, pages = {{202--214}}, publisher = {{Landes Bioscience}}, series = {{Cell Adhesion and Migration}}, title = {{Mast cell tryptase enhances wound healing by promoting migration in human bronchial epithelial cells}}, url = {{http://dx.doi.org/10.1080/19336918.2021.1950594}}, doi = {{10.1080/19336918.2021.1950594}}, volume = {{15}}, year = {{2021}}, }