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Frequency determination of rare populations by flow cytometry: A hematopoietic stem cell perspective.

Rundberg Nilsson, Alexandra LU orcid ; Bryder, David LU and Pronk, Kees-Jan LU (2013) In Cytometry Part A 83A(8). p.721-727
Abstract
Flow cytometry allows for identification of cellular subsets based on cell intrinsic properties, most often by the use of fluorochrome-conjugated antibodies recognizing distinct cell-surface epitopes that define the cells of interest. Advances in technical instrumentation and the availability of an ever-increasing number of fluorophores, today enables identification of multicolor defined cellular populations to a previously unreachable resolution. However, these possibilities put an increasing demand on preparation, acquisition, and subsequent analysis of the investigated samples. Identification of very rare cellular subsets, such as the bone marrow-residing hematopoietic stem cells (HSCs), causes further complexity to such analysis. Here,... (More)
Flow cytometry allows for identification of cellular subsets based on cell intrinsic properties, most often by the use of fluorochrome-conjugated antibodies recognizing distinct cell-surface epitopes that define the cells of interest. Advances in technical instrumentation and the availability of an ever-increasing number of fluorophores, today enables identification of multicolor defined cellular populations to a previously unreachable resolution. However, these possibilities put an increasing demand on preparation, acquisition, and subsequent analysis of the investigated samples. Identification of very rare cellular subsets, such as the bone marrow-residing hematopoietic stem cells (HSCs), causes further complexity to such analysis. Here, we discuss considerations and aspects in multicolor flow cytometry as exemplified by analysis of human and mouse HSCs. We illustrate advantages and drawbacks of polychromatic flow cytometry and propose strategies, such as the use of internal reference populations, for sample analysis. © 2013 International Society for Advancement of Cytometry. (Less)
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publishing date
type
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publication status
published
subject
in
Cytometry Part A
volume
83A
issue
8
pages
721 - 727
publisher
John Wiley & Sons Inc.
external identifiers
  • wos:000330246000007
  • pmid:23839904
  • scopus:84881026735
  • pmid:23839904
ISSN
1552-4930
DOI
10.1002/cyto.a.22324
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Stem Cell Aging (013212073)
id
d9d8cce1-3210-4959-89d0-826a918587a5 (old id 3956040)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/23839904?dopt=Abstract
date added to LUP
2016-04-01 11:14:00
date last changed
2025-04-09 21:33:49
@article{d9d8cce1-3210-4959-89d0-826a918587a5,
  abstract     = {{Flow cytometry allows for identification of cellular subsets based on cell intrinsic properties, most often by the use of fluorochrome-conjugated antibodies recognizing distinct cell-surface epitopes that define the cells of interest. Advances in technical instrumentation and the availability of an ever-increasing number of fluorophores, today enables identification of multicolor defined cellular populations to a previously unreachable resolution. However, these possibilities put an increasing demand on preparation, acquisition, and subsequent analysis of the investigated samples. Identification of very rare cellular subsets, such as the bone marrow-residing hematopoietic stem cells (HSCs), causes further complexity to such analysis. Here, we discuss considerations and aspects in multicolor flow cytometry as exemplified by analysis of human and mouse HSCs. We illustrate advantages and drawbacks of polychromatic flow cytometry and propose strategies, such as the use of internal reference populations, for sample analysis. © 2013 International Society for Advancement of Cytometry.}},
  author       = {{Rundberg Nilsson, Alexandra and Bryder, David and Pronk, Kees-Jan}},
  issn         = {{1552-4930}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{721--727}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Cytometry Part A}},
  title        = {{Frequency determination of rare populations by flow cytometry: A hematopoietic stem cell perspective.}},
  url          = {{http://dx.doi.org/10.1002/cyto.a.22324}},
  doi          = {{10.1002/cyto.a.22324}},
  volume       = {{83A}},
  year         = {{2013}},
}