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Identification and characterization of the ccdA gene, required for cytochrome c synthesis in Bacillus subtilis

Schiött, Torbjörn LU ; von Wachenfeldt, Claes LU and Hederstedt, Lars LU (1997) In Journal of Bacteriology 179(6). p.1962-1973
Abstract
The gram-positive, endospore-forming bacterium Bacillus subtilis contains several membrane-bound c-type cytochromes. We have isolated a mutant pleiotropically deficient in cytochromes c. The responsible mutation resides in a gene which we have named ccdA (cytochrome c defective). This gene is located at 173 degrees on the B. subtilis chromosome. The ccdA gene was found to be specifically required for synthesis of cytochromes of the c type. CcdA is a predicted 26-kDa integral membrane protein with no clear similarity to any known cytochrome c biogenesis protein but seems to be related to a part of Escherichia coli DipZ/DsbD. The ccdA gene is cotranscribed with two other genes. These genes encode a putative 13.5-kDa single-domain response... (More)
The gram-positive, endospore-forming bacterium Bacillus subtilis contains several membrane-bound c-type cytochromes. We have isolated a mutant pleiotropically deficient in cytochromes c. The responsible mutation resides in a gene which we have named ccdA (cytochrome c defective). This gene is located at 173 degrees on the B. subtilis chromosome. The ccdA gene was found to be specifically required for synthesis of cytochromes of the c type. CcdA is a predicted 26-kDa integral membrane protein with no clear similarity to any known cytochrome c biogenesis protein but seems to be related to a part of Escherichia coli DipZ/DsbD. The ccdA gene is cotranscribed with two other genes. These genes encode a putative 13.5-kDa single-domain response regulator, similar to B. subtilis CheY and SpoOF, and a predicted 18-kDa hydrophobic protein with no similarity to any protein in databases, respectively. Inactivation of the three genes showed that only ccdA is required for cytochrome c synthesis. The results also demonstrated that cytochromes of the c type are not needed for growth of B. subtilis. (Less)
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author
organization
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Contribution to journal
publication status
published
subject
in
Journal of Bacteriology
volume
179
issue
6
pages
1962 - 1973
publisher
American Society for Microbiology
ISSN
0021-9193
DOI
10.1128/jb.179.6.1962-1973.1997
language
English
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yes
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d9deeef7-e7ac-4622-b67d-f3efe6e080a0
date added to LUP
2017-07-18 10:02:33
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2017-08-14 11:11:21
@article{d9deeef7-e7ac-4622-b67d-f3efe6e080a0,
  abstract     = {The gram-positive, endospore-forming bacterium Bacillus subtilis contains several membrane-bound c-type cytochromes. We have isolated a mutant pleiotropically deficient in cytochromes c. The responsible mutation resides in a gene which we have named ccdA (cytochrome c defective). This gene is located at 173 degrees on the B. subtilis chromosome. The ccdA gene was found to be specifically required for synthesis of cytochromes of the c type. CcdA is a predicted 26-kDa integral membrane protein with no clear similarity to any known cytochrome c biogenesis protein but seems to be related to a part of Escherichia coli DipZ/DsbD. The ccdA gene is cotranscribed with two other genes. These genes encode a putative 13.5-kDa single-domain response regulator, similar to B. subtilis CheY and SpoOF, and a predicted 18-kDa hydrophobic protein with no similarity to any protein in databases, respectively. Inactivation of the three genes showed that only ccdA is required for cytochrome c synthesis. The results also demonstrated that cytochromes of the c type are not needed for growth of B. subtilis.},
  author       = {Schiött, Torbjörn and von Wachenfeldt, Claes and Hederstedt, Lars},
  issn         = {0021-9193},
  language     = {eng},
  number       = {6},
  pages        = {1962--1973},
  publisher    = {American Society for Microbiology},
  series       = {Journal of Bacteriology},
  title        = {Identification and characterization of the<em> ccdA</em> gene, required for cytochrome c synthesis in <em>Bacillus subtilis</em>},
  url          = {http://dx.doi.org/10.1128/jb.179.6.1962-1973.1997},
  volume       = {179},
  year         = {1997},
}