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miR-204 suppresses the development and progression of human glioblastoma by targeting ATF2

Song, Shiwei; Fajol, Abul LU ; Tu, Xiankun; Ren, Baogang and Shi, Songsheng (2016) In Oncotarget 7(43). p.70058-70065
Abstract

In human cancers, miRNAs are important regulators of multiple cellular processes, and aberrant miRNA expression has been observed, and their alterations contribute to multiple cancer development and progression. Till now, little has been known about the role of miR-204 in human glioblastoma (GBM). In the present study, we used in-vitro assays to investigate the mechanisms of miR-204 in GBM cell lines and 60 cases of GBM tissues. Here, we found that miR-204 expression is downregulated in both GBM cell lines A172, U87 and U251 cells and GBM tissues as compared with NHA cells and normal tissues (all p < 0.001). In addition, the ectopic expression of miR-204 suppressed A172 and U87 cell proliferation, migration and invasion. Meanwhile,... (More)

In human cancers, miRNAs are important regulators of multiple cellular processes, and aberrant miRNA expression has been observed, and their alterations contribute to multiple cancer development and progression. Till now, little has been known about the role of miR-204 in human glioblastoma (GBM). In the present study, we used in-vitro assays to investigate the mechanisms of miR-204 in GBM cell lines and 60 cases of GBM tissues. Here, we found that miR-204 expression is downregulated in both GBM cell lines A172, U87 and U251 cells and GBM tissues as compared with NHA cells and normal tissues (all p < 0.001). In addition, the ectopic expression of miR-204 suppressed A172 and U87 cell proliferation, migration and invasion. Meanwhile, miR-204 over-expression extremely inhibited the protein expression of ATF2. Notably, the enforced expression of ATF2 in A172 and U87 cells with the over-expression of miR-204 attenuated the inhibitory effects of miR-204 on proliferation, migration and invasion. In conclusion, our findings suggest that miR-204 suppressed cell proliferation, migration and invasion through inhibition of ATF2, thus, miR-204 may function as a useful drug target in the treatment and diagnosis of GBM.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
GBM, miR-204, Suppressor
in
Oncotarget
volume
7
issue
43
pages
8 pages
publisher
Impact Journals, LLC
external identifiers
  • scopus:84994323085
  • wos:000387448300073
ISSN
1949-2553
DOI
10.18632/oncotarget.11732
language
English
LU publication?
yes
id
d9eaf6d6-0452-4843-a8ec-8f6c0d29c997
date added to LUP
2016-12-05 09:08:09
date last changed
2017-10-22 05:23:03
@article{d9eaf6d6-0452-4843-a8ec-8f6c0d29c997,
  abstract     = {<p>In human cancers, miRNAs are important regulators of multiple cellular processes, and aberrant miRNA expression has been observed, and their alterations contribute to multiple cancer development and progression. Till now, little has been known about the role of miR-204 in human glioblastoma (GBM). In the present study, we used in-vitro assays to investigate the mechanisms of miR-204 in GBM cell lines and 60 cases of GBM tissues. Here, we found that miR-204 expression is downregulated in both GBM cell lines A172, U87 and U251 cells and GBM tissues as compared with NHA cells and normal tissues (all p &lt; 0.001). In addition, the ectopic expression of miR-204 suppressed A172 and U87 cell proliferation, migration and invasion. Meanwhile, miR-204 over-expression extremely inhibited the protein expression of ATF2. Notably, the enforced expression of ATF2 in A172 and U87 cells with the over-expression of miR-204 attenuated the inhibitory effects of miR-204 on proliferation, migration and invasion. In conclusion, our findings suggest that miR-204 suppressed cell proliferation, migration and invasion through inhibition of ATF2, thus, miR-204 may function as a useful drug target in the treatment and diagnosis of GBM.</p>},
  author       = {Song, Shiwei and Fajol, Abul and Tu, Xiankun and Ren, Baogang and Shi, Songsheng},
  issn         = {1949-2553},
  keyword      = {GBM,miR-204,Suppressor},
  language     = {eng},
  number       = {43},
  pages        = {70058--70065},
  publisher    = {Impact Journals, LLC},
  series       = {Oncotarget},
  title        = {miR-204 suppresses the development and progression of human glioblastoma by targeting ATF2},
  url          = {http://dx.doi.org/10.18632/oncotarget.11732},
  volume       = {7},
  year         = {2016},
}