A general method for bead-enhanced quantitation by flow cytometry
(2006) In Journal of Immunological Methods 317(1-2). p.45-55- Abstract
- Flow cytometry provides accurate relative cellular quantitation (percent abundance) of cells from diverse samples, but technical limitations of most flow cytometers preclude accurate absolute quantitation. Several quantitation standards are now commercially available which, when added to samples, permit absolute quantitation of CD4+ T cells. However, these reagents are limited by their cost, technical complexity, requirement for additional software and/or limited applicability. Moreover, few studies have validated the use of such reagents in complex biological samples, especially for quantitation of non-T cells. Here we show that addition to samples of known quantities of polystyrene fluorescence standardization beads permits accurate... (More)
- Flow cytometry provides accurate relative cellular quantitation (percent abundance) of cells from diverse samples, but technical limitations of most flow cytometers preclude accurate absolute quantitation. Several quantitation standards are now commercially available which, when added to samples, permit absolute quantitation of CD4+ T cells. However, these reagents are limited by their cost, technical complexity, requirement for additional software and/or limited applicability. Moreover, few studies have validated the use of such reagents in complex biological samples, especially for quantitation of non-T cells. Here we show that addition to samples of known quantities of polystyrene fluorescence standardization beads permits accurate quantitation of CD4+ T cells from complex cell samples. This procedure, here termed single bead-enhanced cytofluorimetry (SBEC), was equally capable of enumerating eosinophils as well as subcellular fragments of apoptotic cells, moieties with very different optical and fluorescent characteristics. Relative to other proprietary products, SBEC is simple, inexpensive and requires no special software, suggesting that the method is suitable for the routine quantitation of most cells and other particles by flow cytometry. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1298322
- author
- Montes, Martin ; Jaensson Gyllenbäck, Elin LU ; Orozco, Aaron F ; Lewis, Dorothy E and Corry, David B
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Immunological Methods
- volume
- 317
- issue
- 1-2
- pages
- 45 - 55
- publisher
- Elsevier
- external identifiers
-
- wos:000243148700006
- scopus:33751343683
- pmid:17067632
- ISSN
- 1872-7905
- DOI
- 10.1016/j.jim.2006.09.013
- language
- English
- LU publication?
- no
- id
- da7fd991-2fdb-4029-84ff-0d53e6b3c66f (old id 1298322)
- date added to LUP
- 2016-04-01 15:18:45
- date last changed
- 2022-03-30 00:42:46
@article{da7fd991-2fdb-4029-84ff-0d53e6b3c66f,
abstract = {{Flow cytometry provides accurate relative cellular quantitation (percent abundance) of cells from diverse samples, but technical limitations of most flow cytometers preclude accurate absolute quantitation. Several quantitation standards are now commercially available which, when added to samples, permit absolute quantitation of CD4+ T cells. However, these reagents are limited by their cost, technical complexity, requirement for additional software and/or limited applicability. Moreover, few studies have validated the use of such reagents in complex biological samples, especially for quantitation of non-T cells. Here we show that addition to samples of known quantities of polystyrene fluorescence standardization beads permits accurate quantitation of CD4+ T cells from complex cell samples. This procedure, here termed single bead-enhanced cytofluorimetry (SBEC), was equally capable of enumerating eosinophils as well as subcellular fragments of apoptotic cells, moieties with very different optical and fluorescent characteristics. Relative to other proprietary products, SBEC is simple, inexpensive and requires no special software, suggesting that the method is suitable for the routine quantitation of most cells and other particles by flow cytometry.}},
author = {{Montes, Martin and Jaensson Gyllenbäck, Elin and Orozco, Aaron F and Lewis, Dorothy E and Corry, David B}},
issn = {{1872-7905}},
language = {{eng}},
number = {{1-2}},
pages = {{45--55}},
publisher = {{Elsevier}},
series = {{Journal of Immunological Methods}},
title = {{A general method for bead-enhanced quantitation by flow cytometry}},
url = {{http://dx.doi.org/10.1016/j.jim.2006.09.013}},
doi = {{10.1016/j.jim.2006.09.013}},
volume = {{317}},
year = {{2006}},
}