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Efficient poly(3-hydroxypropionate) production from glycerol using Lactobacillus reuteri and recombinant Escherichia coli harboring L. reuteri propionaldehyde dehydrogenase and Chromobacterium sp. PHA synthase genes.

Linares-Pastén, Javier LU orcid ; Sabet Azad, Ramin LU ; Pessina, Laura LU ; Sardari, Roya R R LU ; Ibrahim, Mohammad H A LU and Hatti-Kaul, Rajni LU (2015) In Bioresource Technology 180. p.172-176
Abstract
Poly(3-hydroxypropionate), P(3HP), is a polymer combining good biodegradability with favorable material properties. In the present study, a production system for P(3HP) was designed, comprising conversion of glycerol to 3-hydroxypropionaldehyde (3HPA) as equilibrium mixture with 3HPA-hydrate and -dimer in aqueous system (reuterin) using resting cells of native Lactobacillus reuteri in a first stage followed by transformation of the 3HPA to P(3HP) using recombinant Escherichia coli strain co-expressing highly active coenzyme A-acylating propionaldehyde dehydrogenase (PduP) from L. reuteri and polyhydroxyalkanoate synthase (PhaCcs) from Chromobacterium sp. P(3HP) content of up to 40% (w/w) cell dry weight was reached, and the yield with... (More)
Poly(3-hydroxypropionate), P(3HP), is a polymer combining good biodegradability with favorable material properties. In the present study, a production system for P(3HP) was designed, comprising conversion of glycerol to 3-hydroxypropionaldehyde (3HPA) as equilibrium mixture with 3HPA-hydrate and -dimer in aqueous system (reuterin) using resting cells of native Lactobacillus reuteri in a first stage followed by transformation of the 3HPA to P(3HP) using recombinant Escherichia coli strain co-expressing highly active coenzyme A-acylating propionaldehyde dehydrogenase (PduP) from L. reuteri and polyhydroxyalkanoate synthase (PhaCcs) from Chromobacterium sp. P(3HP) content of up to 40% (w/w) cell dry weight was reached, and the yield with respect to the reuterin consumed by the cells was 78%. Short biotransformation period (4.5h), lack of additives or expensive cofactors, and use of a cheap medium for cultivation of the recombinant strain, provides a new efficient and potentially economical system for P(3HP) production. (Less)
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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Bioresource Technology
volume
180
pages
172 - 176
publisher
Elsevier
external identifiers
  • pmid:25600014
  • wos:000349715700023
  • scopus:84921261451
  • pmid:25600014
ISSN
1873-2976
DOI
10.1016/j.biortech.2014.12.099
language
English
LU publication?
yes
id
da8e7d19-5b6c-4ec7-8b9f-6e674da6e156 (old id 5040203)
date added to LUP
2016-04-01 10:11:30
date last changed
2022-04-04 03:19:52
@article{da8e7d19-5b6c-4ec7-8b9f-6e674da6e156,
  abstract     = {{Poly(3-hydroxypropionate), P(3HP), is a polymer combining good biodegradability with favorable material properties. In the present study, a production system for P(3HP) was designed, comprising conversion of glycerol to 3-hydroxypropionaldehyde (3HPA) as equilibrium mixture with 3HPA-hydrate and -dimer in aqueous system (reuterin) using resting cells of native Lactobacillus reuteri in a first stage followed by transformation of the 3HPA to P(3HP) using recombinant Escherichia coli strain co-expressing highly active coenzyme A-acylating propionaldehyde dehydrogenase (PduP) from L. reuteri and polyhydroxyalkanoate synthase (PhaCcs) from Chromobacterium sp. P(3HP) content of up to 40% (w/w) cell dry weight was reached, and the yield with respect to the reuterin consumed by the cells was 78%. Short biotransformation period (4.5h), lack of additives or expensive cofactors, and use of a cheap medium for cultivation of the recombinant strain, provides a new efficient and potentially economical system for P(3HP) production.}},
  author       = {{Linares-Pastén, Javier and Sabet Azad, Ramin and Pessina, Laura and Sardari, Roya R R and Ibrahim, Mohammad H A and Hatti-Kaul, Rajni}},
  issn         = {{1873-2976}},
  language     = {{eng}},
  pages        = {{172--176}},
  publisher    = {{Elsevier}},
  series       = {{Bioresource Technology}},
  title        = {{Efficient poly(3-hydroxypropionate) production from glycerol using Lactobacillus reuteri and recombinant Escherichia coli harboring L. reuteri propionaldehyde dehydrogenase and Chromobacterium sp. PHA synthase genes.}},
  url          = {{http://dx.doi.org/10.1016/j.biortech.2014.12.099}},
  doi          = {{10.1016/j.biortech.2014.12.099}},
  volume       = {{180}},
  year         = {{2015}},
}