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Low Incubation Temperature Induces DNA Hypomethylation in Lizard Brains

Paredes, Ursula; Radersma, Reinder LU ; Cannell, Naomi; While, Geoffrey M. and Uller, Tobias LU (2016) In Journal of Experimental Zoology Part A: Ecological Genetics and Physiology 325(6). p.390-395
Abstract

Developmental stress can have organizational effects on suites of physiological, morphological, and behavioral characteristics. In lizards, incubation temperature is perhaps the most significant environmental variable affecting embryonic development. Wall lizards (Podarcis muralis) recently introduced by humans from Italy to England experience stressfully cool incubation conditions, which we here show reduce growth and increase the incidence of scale malformations. Using a methylation-sensitive AFLP protocol optimized for vertebrates, we demonstrate that this low incubation temperature also causes hypomethylation of DNA in brain tissue. A consistent pattern across methylation-susceptible AFLP loci suggests that hypomethylation is a... (More)

Developmental stress can have organizational effects on suites of physiological, morphological, and behavioral characteristics. In lizards, incubation temperature is perhaps the most significant environmental variable affecting embryonic development. Wall lizards (Podarcis muralis) recently introduced by humans from Italy to England experience stressfully cool incubation conditions, which we here show reduce growth and increase the incidence of scale malformations. Using a methylation-sensitive AFLP protocol optimized for vertebrates, we demonstrate that this low incubation temperature also causes hypomethylation of DNA in brain tissue. A consistent pattern across methylation-susceptible AFLP loci suggests that hypomethylation is a general response and not limited to certain CpG sites. The functional consequences of hypomethylation are unknown, but it could contribute to genome stability and regulation of gene expression. Further studies of the effects of incubation temperature on DNA methylation in ectotherm vertebrates may reveal mechanisms that explain why the embryonic thermal environment often has physiological and behavioral consequences for offspring.

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organization
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type
Contribution to journal
publication status
published
subject
in
Journal of Experimental Zoology Part A: Ecological Genetics and Physiology
volume
325
issue
6
pages
6 pages
publisher
John Wiley and Sons Inc.
external identifiers
  • scopus:84978371895
  • wos:000379514100004
ISSN
1932-5223
DOI
10.1002/jez.2024
language
English
LU publication?
yes
id
daf0ef50-205d-4305-a906-d74a89d27bdc
date added to LUP
2017-01-18 09:11:55
date last changed
2017-09-18 11:32:15
@article{daf0ef50-205d-4305-a906-d74a89d27bdc,
  abstract     = {<p>Developmental stress can have organizational effects on suites of physiological, morphological, and behavioral characteristics. In lizards, incubation temperature is perhaps the most significant environmental variable affecting embryonic development. Wall lizards (Podarcis muralis) recently introduced by humans from Italy to England experience stressfully cool incubation conditions, which we here show reduce growth and increase the incidence of scale malformations. Using a methylation-sensitive AFLP protocol optimized for vertebrates, we demonstrate that this low incubation temperature also causes hypomethylation of DNA in brain tissue. A consistent pattern across methylation-susceptible AFLP loci suggests that hypomethylation is a general response and not limited to certain CpG sites. The functional consequences of hypomethylation are unknown, but it could contribute to genome stability and regulation of gene expression. Further studies of the effects of incubation temperature on DNA methylation in ectotherm vertebrates may reveal mechanisms that explain why the embryonic thermal environment often has physiological and behavioral consequences for offspring.</p>},
  author       = {Paredes, Ursula and Radersma, Reinder and Cannell, Naomi and While, Geoffrey M. and Uller, Tobias},
  issn         = {1932-5223},
  language     = {eng},
  month        = {07},
  number       = {6},
  pages        = {390--395},
  publisher    = {John Wiley and Sons Inc.},
  series       = {Journal of Experimental Zoology Part A: Ecological Genetics and Physiology},
  title        = {Low Incubation Temperature Induces DNA Hypomethylation in Lizard Brains},
  url          = {http://dx.doi.org/10.1002/jez.2024},
  volume       = {325},
  year         = {2016},
}