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Complex Formation Between Chymotrypsin and Polymers as a Means to Improve Exposure of the Enzyme to Organic Solvents

Otamiri, Marina ; Adlercreutz, Patrick LU orcid and Mattiasson, Bo LU (1992) In Progress in Biotechnology 8(C). p.363-369
Abstract

When mixing chymotrypsin and a polymer, e.g. ethyl cellulose and drying them together, complexes are formed that are soluble in organic solvents. The enzyme maintains its activity and is even stabilized as compared to a preparation in which the enzyme is added as a dry powder to the solvent. In both cases, minute amounts of water are added afterwards. The system containing a polymer shows a markedly reduced sensitivity to additions of water to the system. The complex formation is reversible and the enzyme can conveniently be recovered from the organic solvent by adding enough water to form a separate phase.

Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
host publication
Progress in Biotechnology
series title
Progress in Biotechnology
volume
8
issue
C
edition
C
pages
7 pages
external identifiers
  • scopus:85013530383
ISSN
0921-0423
DOI
10.1016/B978-0-444-89046-7.50056-4
language
English
LU publication?
yes
id
db1dbdec-abdf-461c-9048-a543a16c5c67
date added to LUP
2019-06-22 09:23:35
date last changed
2021-09-26 03:01:06
@inbook{db1dbdec-abdf-461c-9048-a543a16c5c67,
  abstract     = {{<p>When mixing chymotrypsin and a polymer, e.g. ethyl cellulose and drying them together, complexes are formed that are soluble in organic solvents. The enzyme maintains its activity and is even stabilized as compared to a preparation in which the enzyme is added as a dry powder to the solvent. In both cases, minute amounts of water are added afterwards. The system containing a polymer shows a markedly reduced sensitivity to additions of water to the system. The complex formation is reversible and the enzyme can conveniently be recovered from the organic solvent by adding enough water to form a separate phase.</p>}},
  author       = {{Otamiri, Marina and Adlercreutz, Patrick and Mattiasson, Bo}},
  booktitle    = {{Progress in Biotechnology}},
  issn         = {{0921-0423}},
  language     = {{eng}},
  number       = {{C}},
  pages        = {{363--369}},
  series       = {{Progress in Biotechnology}},
  title        = {{Complex Formation Between Chymotrypsin and Polymers as a Means to Improve Exposure of the Enzyme to Organic Solvents}},
  url          = {{http://dx.doi.org/10.1016/B978-0-444-89046-7.50056-4}},
  doi          = {{10.1016/B978-0-444-89046-7.50056-4}},
  volume       = {{8}},
  year         = {{1992}},
}