Complex Formation Between Chymotrypsin and Polymers as a Means to Improve Exposure of the Enzyme to Organic Solvents
(1992) In Progress in Biotechnology 8(C). p.363-369- Abstract
When mixing chymotrypsin and a polymer, e.g. ethyl cellulose and drying them together, complexes are formed that are soluble in organic solvents. The enzyme maintains its activity and is even stabilized as compared to a preparation in which the enzyme is added as a dry powder to the solvent. In both cases, minute amounts of water are added afterwards. The system containing a polymer shows a markedly reduced sensitivity to additions of water to the system. The complex formation is reversible and the enzyme can conveniently be recovered from the organic solvent by adding enough water to form a separate phase.
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- author
- Otamiri, Marina ; Adlercreutz, Patrick LU and Mattiasson, Bo LU
- organization
- publishing date
- 1992
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- host publication
- Progress in Biotechnology
- series title
- Progress in Biotechnology
- volume
- 8
- issue
- C
- edition
- C
- pages
- 7 pages
- external identifiers
-
- scopus:85013530383
- ISSN
- 0921-0423
- DOI
- 10.1016/B978-0-444-89046-7.50056-4
- language
- English
- LU publication?
- yes
- id
- db1dbdec-abdf-461c-9048-a543a16c5c67
- date added to LUP
- 2019-06-22 09:23:35
- date last changed
- 2021-09-26 03:01:06
@inbook{db1dbdec-abdf-461c-9048-a543a16c5c67, abstract = {{<p>When mixing chymotrypsin and a polymer, e.g. ethyl cellulose and drying them together, complexes are formed that are soluble in organic solvents. The enzyme maintains its activity and is even stabilized as compared to a preparation in which the enzyme is added as a dry powder to the solvent. In both cases, minute amounts of water are added afterwards. The system containing a polymer shows a markedly reduced sensitivity to additions of water to the system. The complex formation is reversible and the enzyme can conveniently be recovered from the organic solvent by adding enough water to form a separate phase.</p>}}, author = {{Otamiri, Marina and Adlercreutz, Patrick and Mattiasson, Bo}}, booktitle = {{Progress in Biotechnology}}, issn = {{0921-0423}}, language = {{eng}}, number = {{C}}, pages = {{363--369}}, series = {{Progress in Biotechnology}}, title = {{Complex Formation Between Chymotrypsin and Polymers as a Means to Improve Exposure of the Enzyme to Organic Solvents}}, url = {{http://dx.doi.org/10.1016/B978-0-444-89046-7.50056-4}}, doi = {{10.1016/B978-0-444-89046-7.50056-4}}, volume = {{8}}, year = {{1992}}, }