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Stroke alters behavior of human skin-derived neural progenitors after transplantation adjacent to neurogenic area in rat brain

De La Rosa-Prieto, Carlos; Laterza, Cecilia LU ; Gonzalez-Ramos, Ana; Wattananit, Somsak LU ; Ge, Ruimin LU ; Lindvall, Olle LU ; Tornero, Daniel LU and Kokaia, Zaal LU (2017) In Stem Cell Research and Therapy 8(1).
Abstract

Background: Intracerebral transplantation of human induced pluripotent stem cells (iPSCs) can ameliorate behavioral deficits in animal models of stroke. How the ischemic lesion affects the survival of the transplanted cells, their proliferation, migration, differentiation, and function is only partly understood. Methods: Here we have assessed the influence of the stroke-induced injury on grafts of human skin iPSCs-derived long-term neuroepithelial-like stem cells using transplantation into the rostral migratory stream (RMS), adjacent to the neurogenic subventricular zone, in adult rats as a model system. Results: We show that the occurrence of an ischemic lesion, induced by middle cerebral artery occlusion, in the striatum close to the... (More)

Background: Intracerebral transplantation of human induced pluripotent stem cells (iPSCs) can ameliorate behavioral deficits in animal models of stroke. How the ischemic lesion affects the survival of the transplanted cells, their proliferation, migration, differentiation, and function is only partly understood. Methods: Here we have assessed the influence of the stroke-induced injury on grafts of human skin iPSCs-derived long-term neuroepithelial-like stem cells using transplantation into the rostral migratory stream (RMS), adjacent to the neurogenic subventricular zone, in adult rats as a model system. Results: We show that the occurrence of an ischemic lesion, induced by middle cerebral artery occlusion, in the striatum close to the transplant does not alter the survival, proliferation, or generation of neuroblasts or mature neurons or astrocytes from the grafted progenitors. In contrast, the migration and axonal projection patterns of the transplanted cells are markedly influenced. In the intact brain, the grafted cells send many fibers to the main olfactory bulb through the RMS and a few of them migrate in the same direction, reaching the first one third of this pathway. In the stroke-injured brain, on the other hand, the grafted cells only migrate toward the ischemic lesion and virtually no axonal outgrowth is observed in the RMS. Conclusions: Our findings indicate that signals released from the stroke-injured area regulate the migration of and fiber outgrowth from grafted human skin-derived neural progenitors and overcome the influence on these cellular properties exerted by the neurogenic area/RMS in the intact brain.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Adult neurogenesis, Human skin-derived cells, Pluripotent, Rostral migratory stream, Stroke, Subventricular zone, Transplantation
in
Stem Cell Research and Therapy
volume
8
issue
1
publisher
BioMed Central
external identifiers
  • scopus:85015071356
  • wos:000396977700010
ISSN
1757-6512
DOI
10.1186/s13287-017-0513-6
language
English
LU publication?
yes
id
dc3da5ec-cbed-4e71-a059-faf1de6f0dd8
date added to LUP
2017-04-10 13:44:56
date last changed
2017-09-18 13:31:51
@article{dc3da5ec-cbed-4e71-a059-faf1de6f0dd8,
  abstract     = {<p>Background: Intracerebral transplantation of human induced pluripotent stem cells (iPSCs) can ameliorate behavioral deficits in animal models of stroke. How the ischemic lesion affects the survival of the transplanted cells, their proliferation, migration, differentiation, and function is only partly understood. Methods: Here we have assessed the influence of the stroke-induced injury on grafts of human skin iPSCs-derived long-term neuroepithelial-like stem cells using transplantation into the rostral migratory stream (RMS), adjacent to the neurogenic subventricular zone, in adult rats as a model system. Results: We show that the occurrence of an ischemic lesion, induced by middle cerebral artery occlusion, in the striatum close to the transplant does not alter the survival, proliferation, or generation of neuroblasts or mature neurons or astrocytes from the grafted progenitors. In contrast, the migration and axonal projection patterns of the transplanted cells are markedly influenced. In the intact brain, the grafted cells send many fibers to the main olfactory bulb through the RMS and a few of them migrate in the same direction, reaching the first one third of this pathway. In the stroke-injured brain, on the other hand, the grafted cells only migrate toward the ischemic lesion and virtually no axonal outgrowth is observed in the RMS. Conclusions: Our findings indicate that signals released from the stroke-injured area regulate the migration of and fiber outgrowth from grafted human skin-derived neural progenitors and overcome the influence on these cellular properties exerted by the neurogenic area/RMS in the intact brain.</p>},
  articleno    = {59},
  author       = {De La Rosa-Prieto, Carlos and Laterza, Cecilia and Gonzalez-Ramos, Ana and Wattananit, Somsak and Ge, Ruimin and Lindvall, Olle and Tornero, Daniel and Kokaia, Zaal},
  issn         = {1757-6512},
  keyword      = {Adult neurogenesis,Human skin-derived cells,Pluripotent,Rostral migratory stream,Stroke,Subventricular zone,Transplantation},
  language     = {eng},
  month        = {03},
  number       = {1},
  publisher    = {BioMed Central},
  series       = {Stem Cell Research and Therapy},
  title        = {Stroke alters behavior of human skin-derived neural progenitors after transplantation adjacent to neurogenic area in rat brain},
  url          = {http://dx.doi.org/10.1186/s13287-017-0513-6},
  volume       = {8},
  year         = {2017},
}