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Continuous multicomponent quantification during supercritical fluid extraction applied to microalgae using in-line UV/Vis absorption spectroscopy and on-line evaporative light scattering detection

Abrahamsson, Victor LU ; Jumaah, Firas LU and Turner, Charlotta LU (2018) In Journal of Supercritical Fluids 131. p.157-165
Abstract

A quantitative methodology based on in-line UV/Vis absorption spectroscopy and on-line evaporative light scattering detection for supercritical fluid extraction is proposed. The method was applied to the extraction of carotenoids, chlorophyll A, ergosterol and total lipids from microalgae. One regression technique and two curve resolution techniques were applied on the absorption spectroscopy data and evaluated, namely classical least squares, multivariate curve resolution by alternating least squares and parallel factor analysis (PARAFAC2). The two former both generated useful models, furthermore multivariate curve resolution also successfully enabled estimation of both spectra and concentration profiles of the analytes. The integrated... (More)

A quantitative methodology based on in-line UV/Vis absorption spectroscopy and on-line evaporative light scattering detection for supercritical fluid extraction is proposed. The method was applied to the extraction of carotenoids, chlorophyll A, ergosterol and total lipids from microalgae. One regression technique and two curve resolution techniques were applied on the absorption spectroscopy data and evaluated, namely classical least squares, multivariate curve resolution by alternating least squares and parallel factor analysis (PARAFAC2). The two former both generated useful models, furthermore multivariate curve resolution also successfully enabled estimation of both spectra and concentration profiles of the analytes. The integrated extraction profiles of each analyte were compared with analysis of the collected fractions using reference analysis methods Precision, in regards to quantification of the analytes in the eluent, was better using in-line measurements compared to off-line measurements by UV/Vis absorption spectroscopy, supercritical fluid chromatography with mass spectrometry and liquid chromatography with UV/Vis detection.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Analytical method, Carotenoids, Chromatography, Microalgae, SFC, SFE
in
Journal of Supercritical Fluids
volume
131
pages
9 pages
publisher
Elsevier
external identifiers
  • scopus:85029473786
ISSN
0896-8446
DOI
10.1016/j.supflu.2017.09.014
language
English
LU publication?
yes
id
dc54775b-764c-4285-98e3-a90e84f84b65
date added to LUP
2017-09-28 14:52:07
date last changed
2018-01-07 12:19:56
@article{dc54775b-764c-4285-98e3-a90e84f84b65,
  abstract     = {<p>A quantitative methodology based on in-line UV/Vis absorption spectroscopy and on-line evaporative light scattering detection for supercritical fluid extraction is proposed. The method was applied to the extraction of carotenoids, chlorophyll A, ergosterol and total lipids from microalgae. One regression technique and two curve resolution techniques were applied on the absorption spectroscopy data and evaluated, namely classical least squares, multivariate curve resolution by alternating least squares and parallel factor analysis (PARAFAC2). The two former both generated useful models, furthermore multivariate curve resolution also successfully enabled estimation of both spectra and concentration profiles of the analytes. The integrated extraction profiles of each analyte were compared with analysis of the collected fractions using reference analysis methods Precision, in regards to quantification of the analytes in the eluent, was better using in-line measurements compared to off-line measurements by UV/Vis absorption spectroscopy, supercritical fluid chromatography with mass spectrometry and liquid chromatography with UV/Vis detection.</p>},
  author       = {Abrahamsson, Victor and Jumaah, Firas and Turner, Charlotta},
  issn         = {0896-8446},
  keyword      = {Analytical method,Carotenoids,Chromatography,Microalgae,SFC,SFE},
  language     = {eng},
  pages        = {157--165},
  publisher    = {Elsevier},
  series       = {Journal of Supercritical Fluids},
  title        = {Continuous multicomponent quantification during supercritical fluid extraction applied to microalgae using in-line UV/Vis absorption spectroscopy and on-line evaporative light scattering detection},
  url          = {http://dx.doi.org/10.1016/j.supflu.2017.09.014},
  volume       = {131},
  year         = {2018},
}