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Cdc42 is involved in PKCepsilon- and delta-induced neurite outgrowth and stress fibre dismantling.

Trollér, Ulrika LU and Larsson, Christer LU (2006) In Biochemical and Biophysical Research Communications 349(1). p.91-98
Abstract
We have shown that protein kinase C (PKC)epsilon, independently of the catalytic domain, induces outgrowth of cellular processes via its regulatory domain in both neural cells and fibroblasts. This was accompanied by stress fibre loss. Here, we have examined the role of the small GTPases, Rac1, and Cdc42, in these PKC-mediated morphological and cytoskeletal changes. Both constitutively active and dominant negative Rac1 and Cdc42 attenuated the PKC-mediated outgrowth of processes. The suppression was larger for Cdc42 than for Rac1. The PKC-mediated dismantling of the stress fibres in both HiB5 and fibroblasts was inhibited by the expression of the Cdc42 mutants whereas they had smaller effects on the stress fibre dismantling induced by the... (More)
We have shown that protein kinase C (PKC)epsilon, independently of the catalytic domain, induces outgrowth of cellular processes via its regulatory domain in both neural cells and fibroblasts. This was accompanied by stress fibre loss. Here, we have examined the role of the small GTPases, Rac1, and Cdc42, in these PKC-mediated morphological and cytoskeletal changes. Both constitutively active and dominant negative Rac1 and Cdc42 attenuated the PKC-mediated outgrowth of processes. The suppression was larger for Cdc42 than for Rac1. The PKC-mediated dismantling of the stress fibres in both HiB5 and fibroblasts was inhibited by the expression of the Cdc42 mutants whereas they had smaller effects on the stress fibre dismantling induced by the ROCK inhibitor, Y-27632, indicating a more crucial role for Cdc42 in the PKC-mediated pathway. We conclude that Cdc42 is an important downstream factor in the pathway through which PKC mediates morphological and cytoskeletal effects. (c) 2006 Elsevier Inc. All rights reserved. (Less)
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Contribution to journal
publication status
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keywords
ROCK, protein kinase C, stress fibres, neuroblastoma, cells, Rac1, fibroblasts, neurite outgrowth, Cdc42
in
Biochemical and Biophysical Research Communications
volume
349
issue
1
pages
91 - 98
publisher
Elsevier
external identifiers
  • pmid:16930532
  • wos:000240650000012
  • scopus:33748324660
  • pmid:16930532
ISSN
1090-2104
DOI
10.1016/j.bbrc.2006.07.200
language
English
LU publication?
yes
id
dc6dbb4d-5fa6-4ff3-ac40-3ef1f218dd26 (old id 159901)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16930532&dopt=Abstract
date added to LUP
2016-04-01 16:53:22
date last changed
2020-01-12 19:50:24
@article{dc6dbb4d-5fa6-4ff3-ac40-3ef1f218dd26,
  abstract     = {We have shown that protein kinase C (PKC)epsilon, independently of the catalytic domain, induces outgrowth of cellular processes via its regulatory domain in both neural cells and fibroblasts. This was accompanied by stress fibre loss. Here, we have examined the role of the small GTPases, Rac1, and Cdc42, in these PKC-mediated morphological and cytoskeletal changes. Both constitutively active and dominant negative Rac1 and Cdc42 attenuated the PKC-mediated outgrowth of processes. The suppression was larger for Cdc42 than for Rac1. The PKC-mediated dismantling of the stress fibres in both HiB5 and fibroblasts was inhibited by the expression of the Cdc42 mutants whereas they had smaller effects on the stress fibre dismantling induced by the ROCK inhibitor, Y-27632, indicating a more crucial role for Cdc42 in the PKC-mediated pathway. We conclude that Cdc42 is an important downstream factor in the pathway through which PKC mediates morphological and cytoskeletal effects. (c) 2006 Elsevier Inc. All rights reserved.},
  author       = {Trollér, Ulrika and Larsson, Christer},
  issn         = {1090-2104},
  language     = {eng},
  number       = {1},
  pages        = {91--98},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {Cdc42 is involved in PKCepsilon- and delta-induced neurite outgrowth and stress fibre dismantling.},
  url          = {http://dx.doi.org/10.1016/j.bbrc.2006.07.200},
  doi          = {10.1016/j.bbrc.2006.07.200},
  volume       = {349},
  year         = {2006},
}