Xenobiotic Pathway Gene Polymorphisms Associated with Gastric Cancer in High Risk Mizo-Mongoloid Population, Northeast India
(2016) In Helicobacter 21(6). p.523-535- Abstract
Background: The aim of this study was to evaluate the risk of gastric cancer associated with individual or combined glutathione S-transferases (GSTs) polymorphism and their interaction with environmental factors. Materials and Methods: Genotyping by PCR was carried out for 80 cases and controls each for GSTM1, GSTT1, and GSTP1 polymorphism and mapped for gene—environment association studies. The samples were subjected to pathogen detection and GSTP1 expression for analyzing their association with different genotypes. Logistic regression analyses were conducted to compute the influence of both genetic and environmental factors for gastric cancer. MDR analysis was performed to assess the risk of gastric cancer by studying the gene–gene... (More)
Background: The aim of this study was to evaluate the risk of gastric cancer associated with individual or combined glutathione S-transferases (GSTs) polymorphism and their interaction with environmental factors. Materials and Methods: Genotyping by PCR was carried out for 80 cases and controls each for GSTM1, GSTT1, and GSTP1 polymorphism and mapped for gene—environment association studies. The samples were subjected to pathogen detection and GSTP1 expression for analyzing their association with different genotypes. Logistic regression analyses were conducted to compute the influence of both genetic and environmental factors for gastric cancer. MDR analysis was performed to assess the risk of gastric cancer by studying the gene–gene and gene–environment effect on the basis of GST genotyping and GSTP1 gene expression. Results: Infection with Helicobacter pylori and CagA+ strains was more frequent in patients with GSTM1/T1 null genotype. Intake of high fermented fat and smoked meat was found to be significantly associated with gastric cancer. The G/G, A/G (rs1695), and T/T (rs1138272) were found to be significantly associated with low expression of GSTP1 gene in cancer tissue. Conclusion: Presence of H. pylori with CagA genotype showed significant individual effect with GSTT1 polymorphism as well as strong synergistic effect in gastric cancer risk. Majority of the gastric cancer samples showed significant negative expression in G/G, A/G (rs1695), and T/T (rs1138272) genotypes. This study shows that GST gene polymorphism was significantly relevant for determining the individual susceptibility to gastric cancer.
(Less)
- author
- Ghatak, Souvik LU ; Yadav, Ravi Prakash ; Lalrohlui, Freda ; Chakraborty, Payel LU ; Ghosh, Soumee ; Ghosh, Sudakshina ; Das, Madhusudan ; Pautu, Jeremy L. ; Zohmingthanga, John and Senthil Kumar, Nachimuthu
- publishing date
- 2016-12-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- CagA, Gastric cancer, glutathione S-transferases, Helicobacter pylori, Mizo-Mongoloid, polymorphisms
- in
- Helicobacter
- volume
- 21
- issue
- 6
- pages
- 523 - 535
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:84961813303
- pmid:27006283
- ISSN
- 1083-4389
- DOI
- 10.1111/hel.12308
- language
- English
- LU publication?
- no
- additional info
- Publisher Copyright: © 2016 John Wiley & Sons Ltd
- id
- dc9115bf-e2be-435f-ad75-1319f287d022
- date added to LUP
- 2021-11-09 16:59:44
- date last changed
- 2024-05-04 17:12:19
@article{dc9115bf-e2be-435f-ad75-1319f287d022,
abstract = {{<p>Background: The aim of this study was to evaluate the risk of gastric cancer associated with individual or combined glutathione S-transferases (GSTs) polymorphism and their interaction with environmental factors. Materials and Methods: Genotyping by PCR was carried out for 80 cases and controls each for GSTM1, GSTT1, and GSTP1 polymorphism and mapped for gene—environment association studies. The samples were subjected to pathogen detection and GSTP1 expression for analyzing their association with different genotypes. Logistic regression analyses were conducted to compute the influence of both genetic and environmental factors for gastric cancer. MDR analysis was performed to assess the risk of gastric cancer by studying the gene–gene and gene–environment effect on the basis of GST genotyping and GSTP1 gene expression. Results: Infection with Helicobacter pylori and CagA+ strains was more frequent in patients with GSTM1/T1 null genotype. Intake of high fermented fat and smoked meat was found to be significantly associated with gastric cancer. The G/G, A/G (rs1695), and T/T (rs1138272) were found to be significantly associated with low expression of GSTP1 gene in cancer tissue. Conclusion: Presence of H. pylori with CagA genotype showed significant individual effect with GSTT1 polymorphism as well as strong synergistic effect in gastric cancer risk. Majority of the gastric cancer samples showed significant negative expression in G/G, A/G (rs1695), and T/T (rs1138272) genotypes. This study shows that GST gene polymorphism was significantly relevant for determining the individual susceptibility to gastric cancer.</p>}},
author = {{Ghatak, Souvik and Yadav, Ravi Prakash and Lalrohlui, Freda and Chakraborty, Payel and Ghosh, Soumee and Ghosh, Sudakshina and Das, Madhusudan and Pautu, Jeremy L. and Zohmingthanga, John and Senthil Kumar, Nachimuthu}},
issn = {{1083-4389}},
keywords = {{CagA; Gastric cancer; glutathione S-transferases; Helicobacter pylori; Mizo-Mongoloid; polymorphisms}},
language = {{eng}},
month = {{12}},
number = {{6}},
pages = {{523--535}},
publisher = {{Wiley-Blackwell}},
series = {{Helicobacter}},
title = {{Xenobiotic Pathway Gene Polymorphisms Associated with Gastric Cancer in High Risk Mizo-Mongoloid Population, Northeast India}},
url = {{http://dx.doi.org/10.1111/hel.12308}},
doi = {{10.1111/hel.12308}},
volume = {{21}},
year = {{2016}},
}