Foodborne pathogens in unpasteurized milk in Sweden
(2018) In International Journal of Food Microbiology 284. p.120-127- Abstract
- Raw milk may be a risk for public health if it is contaminated with zoonotic pathogens. To study the prevalence
in unpasteurized milk from Swedish farms, bovine and small ruminant dairy farms were sampled. Since the
sampling method and transport conditions may influence the outcome of analyses, efforts were made to optimize
the methodology. Culturing of bacteria was done from in-line milk filters collected from the milk pipe at the
point where it enters the milk bulk tank at the farms and this way of sampling was compared to sampling bulk
tank milk (BTM) directly. Analysing milk filters were found to be superior to analysing BTM directly. Conditions
for transport of milk filter samples were further improved by the... (More) - Raw milk may be a risk for public health if it is contaminated with zoonotic pathogens. To study the prevalence
in unpasteurized milk from Swedish farms, bovine and small ruminant dairy farms were sampled. Since the
sampling method and transport conditions may influence the outcome of analyses, efforts were made to optimize
the methodology. Culturing of bacteria was done from in-line milk filters collected from the milk pipe at the
point where it enters the milk bulk tank at the farms and this way of sampling was compared to sampling bulk
tank milk (BTM) directly. Analysing milk filters were found to be superior to analysing BTM directly. Conditions
for transport of milk filter samples were further improved by the addition of Cary Blair transport medium, which
significantly increased the number of positive samples for pathogenic bacteria. The isolation of several foodborne
pathogens from milk filters was demonstrated. The prevalence of samples with Staphylococcus aureus was
71% and 64%, and Listeria spp. 21% and 29% from dairy cow and goat/sheep farms, respectively. Campylobacter
jejuni, Yersinia enterocolitica and verotoxigenic Escherichia coli (VTEC) O157 were detected in 9%, 2% and 2% of
samples from bovine milk, respectively.
We conclude that the choice of sampling method and sample handling influence the results of bacterial
culturing. From the results of this study, we strongly recommend to sample in-line milk filters instead of BTM
directly and to use Cary Blair medium during transport, especially if the samples are to be analysed for
Campylobacter spp. and/or Listeria spp. The findings also show that unpasteurized milk from Swedish farms
occasionally contain bacteria with zoonotic potential. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/dca9cef0-25ab-42c4-8148-8de7c13fe391
- author
- Artursson, Karin ; Schelin, Jenny LU ; Thisted Lambertz, Susanne ; Hansson, Ingrid and Olsson Engvall, Eva
- organization
- publishing date
- 2018-05-19
- type
- Contribution to journal
- publication status
- published
- subject
- in
- International Journal of Food Microbiology
- volume
- 284
- pages
- 120 - 127
- publisher
- Elsevier
- external identifiers
-
- scopus:85048122529
- pmid:29887505
- ISSN
- 0168-1605
- DOI
- 10.1016/j.ijfoodmicro.2018.05.015
- language
- English
- LU publication?
- yes
- id
- dca9cef0-25ab-42c4-8148-8de7c13fe391
- date added to LUP
- 2018-06-11 15:49:12
- date last changed
- 2022-04-02 00:43:22
@article{dca9cef0-25ab-42c4-8148-8de7c13fe391,
abstract = {{Raw milk may be a risk for public health if it is contaminated with zoonotic pathogens. To study the prevalence<br/>in unpasteurized milk from Swedish farms, bovine and small ruminant dairy farms were sampled. Since the<br/>sampling method and transport conditions may influence the outcome of analyses, efforts were made to optimize<br/>the methodology. Culturing of bacteria was done from in-line milk filters collected from the milk pipe at the<br/>point where it enters the milk bulk tank at the farms and this way of sampling was compared to sampling bulk<br/>tank milk (BTM) directly. Analysing milk filters were found to be superior to analysing BTM directly. Conditions<br/>for transport of milk filter samples were further improved by the addition of Cary Blair transport medium, which<br/>significantly increased the number of positive samples for pathogenic bacteria. The isolation of several foodborne<br/>pathogens from milk filters was demonstrated. The prevalence of samples with Staphylococcus aureus was<br/>71% and 64%, and Listeria spp. 21% and 29% from dairy cow and goat/sheep farms, respectively. Campylobacter<br/>jejuni, Yersinia enterocolitica and verotoxigenic Escherichia coli (VTEC) O157 were detected in 9%, 2% and 2% of<br/>samples from bovine milk, respectively.<br/>We conclude that the choice of sampling method and sample handling influence the results of bacterial<br/>culturing. From the results of this study, we strongly recommend to sample in-line milk filters instead of BTM<br/>directly and to use Cary Blair medium during transport, especially if the samples are to be analysed for<br/>Campylobacter spp. and/or Listeria spp. The findings also show that unpasteurized milk from Swedish farms<br/>occasionally contain bacteria with zoonotic potential.}},
author = {{Artursson, Karin and Schelin, Jenny and Thisted Lambertz, Susanne and Hansson, Ingrid and Olsson Engvall, Eva}},
issn = {{0168-1605}},
language = {{eng}},
month = {{05}},
pages = {{120--127}},
publisher = {{Elsevier}},
series = {{International Journal of Food Microbiology}},
title = {{Foodborne pathogens in unpasteurized milk in Sweden}},
url = {{http://dx.doi.org/10.1016/j.ijfoodmicro.2018.05.015}},
doi = {{10.1016/j.ijfoodmicro.2018.05.015}},
volume = {{284}},
year = {{2018}},
}