Tissue deposits of IgA-binding streptococcal M proteins in IgA nephropathy and Henoch-Schonlein purpura.
Schmitt, Roland LU ; Carlsson, Fredric LU ; Mörgelin, Matthias LU ; Tati, Ramesh LU ; Lindahl, Gunnar LU and Karpman, Diana LU
(2010)
In American Journal of Pathology
176(2).
p.608-618
- Abstract
- IgA nephropathy (IgAN) and Henoch-Schönlein purpura (HSP) are diseases characterized by IgA deposits in the kidney and/or skin. Both may arise after upper respiratory tract infections, but the pathogenic mechanisms governing these diseases remain unclear. Patients with IgAN (n = 16) and HSP (n = 17) were included in this study aimed at examining whether IgA-binding M proteins of group A streptococci could be involved. As M proteins vary in sequence, the study focused on the IgA-binding-region (IgA-BR) of three different M proteins: M4, M22, and M60. Renal tissue from IgAN and HSP patients and skin from HSP patients were examined for deposits of streptococcal IgA-BR by immunohistochemistry and electron microscopy using specific antibodies,... (More)
- IgA nephropathy (IgAN) and Henoch-Schönlein purpura (HSP) are diseases characterized by IgA deposits in the kidney and/or skin. Both may arise after upper respiratory tract infections, but the pathogenic mechanisms governing these diseases remain unclear. Patients with IgAN (n = 16) and HSP (n = 17) were included in this study aimed at examining whether IgA-binding M proteins of group A streptococci could be involved. As M proteins vary in sequence, the study focused on the IgA-binding-region (IgA-BR) of three different M proteins: M4, M22, and M60. Renal tissue from IgAN and HSP patients and skin from HSP patients were examined for deposits of streptococcal IgA-BR by immunohistochemistry and electron microscopy using specific antibodies, and a skin sample from a HSP patient was examined by mass spectrometry. IgA-BR deposits were detected in 10/16 IgAN kidneys and 7/13 HSP kidneys. Electron microscopy demonstrated deposits of IgA-BRs in the mesangial matrix and glomerular basement membrane, which colocalized with IgA. Skin samples exhibited IgA-BR deposits in 4/5 biopsies, a result confirmed by mass spectrometry in one patient. IgA-BR deposits were not detected in normal kidney and skin samples. Taken together, these results demonstrate IgA-BR from streptococcal M proteins in patient tissues. IgA-BR, would on gaining access to the circulation, encounter circulatory IgA and form a complex with IgA-Fc that could deposit in tissues and contribute to the pathogenesis of IgAN and HSP. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1541336
- author
- Schmitt, Roland
LU
; Carlsson, Fredric
LU
; Mörgelin, Matthias
LU
; Tati, Ramesh
LU
; Lindahl, Gunnar
LU
and Karpman, Diana
LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- American Journal of Pathology
- volume
- 176
- issue
- 2
- pages
- 608 - 618
- publisher
- American Society for Investigative Pathology
- external identifiers
-
- wos:000274111400012
- pmid:20056836
- scopus:76149095207
- pmid:20056836
- ISSN
- 1525-2191
- DOI
- 10.2353/ajpath.2010.090428
- language
- English
- LU publication?
- yes
- id
- dcb350d8-6c99-4bce-bcf2-6efeaac95888 (old id 1541336)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/20056836?dopt=Abstract
- date added to LUP
- 2016-04-04 08:35:54
- date last changed
- 2022-02-20 21:56:11
@article{dcb350d8-6c99-4bce-bcf2-6efeaac95888,
abstract = {{IgA nephropathy (IgAN) and Henoch-Schönlein purpura (HSP) are diseases characterized by IgA deposits in the kidney and/or skin. Both may arise after upper respiratory tract infections, but the pathogenic mechanisms governing these diseases remain unclear. Patients with IgAN (n = 16) and HSP (n = 17) were included in this study aimed at examining whether IgA-binding M proteins of group A streptococci could be involved. As M proteins vary in sequence, the study focused on the IgA-binding-region (IgA-BR) of three different M proteins: M4, M22, and M60. Renal tissue from IgAN and HSP patients and skin from HSP patients were examined for deposits of streptococcal IgA-BR by immunohistochemistry and electron microscopy using specific antibodies, and a skin sample from a HSP patient was examined by mass spectrometry. IgA-BR deposits were detected in 10/16 IgAN kidneys and 7/13 HSP kidneys. Electron microscopy demonstrated deposits of IgA-BRs in the mesangial matrix and glomerular basement membrane, which colocalized with IgA. Skin samples exhibited IgA-BR deposits in 4/5 biopsies, a result confirmed by mass spectrometry in one patient. IgA-BR deposits were not detected in normal kidney and skin samples. Taken together, these results demonstrate IgA-BR from streptococcal M proteins in patient tissues. IgA-BR, would on gaining access to the circulation, encounter circulatory IgA and form a complex with IgA-Fc that could deposit in tissues and contribute to the pathogenesis of IgAN and HSP.}},
author = {{Schmitt, Roland and Carlsson, Fredric and Mörgelin, Matthias and Tati, Ramesh and Lindahl, Gunnar and Karpman, Diana}},
issn = {{1525-2191}},
language = {{eng}},
number = {{2}},
pages = {{608--618}},
publisher = {{American Society for Investigative Pathology}},
series = {{American Journal of Pathology}},
title = {{Tissue deposits of IgA-binding streptococcal M proteins in IgA nephropathy and Henoch-Schonlein purpura.}},
url = {{http://dx.doi.org/10.2353/ajpath.2010.090428}},
doi = {{10.2353/ajpath.2010.090428}},
volume = {{176}},
year = {{2010}},
}