In vitro preparation of amelogenin nanoparticles carrying nucleic acids.
Svensson Bonde, Johan LU
and Bülow, Leif
LU
(2014)
In Biotechnology Letters
36(6).
p.1349-1357
- Abstract
- Amelogenin, a matrix protein involved in biomineralization of enamel, can self-assemble to form nanospheres in a pH-dependent manner. Nucleic acids (single-stranded, double-stranded, and plasmid DNA, as well as RNA) could be co-precipitated with amelogenin, demonstrating a strong binding of nucleic acids to amelogenin. The amounts of co-precipitated nucleic acids were analyzed and binding levels upto 90 μg DNA/mg amelogenin was achieved. The co-precipitation could also be carried out in a bacterial cell homogenate, and no bacterial proteins were found in the amelogenin aggregates, suggesting specificity for nucleic acid binding. Dynamic light scattering showed that amelogenin nanosphere structure is maintained upon DNA binding with an upto... (More)
- Amelogenin, a matrix protein involved in biomineralization of enamel, can self-assemble to form nanospheres in a pH-dependent manner. Nucleic acids (single-stranded, double-stranded, and plasmid DNA, as well as RNA) could be co-precipitated with amelogenin, demonstrating a strong binding of nucleic acids to amelogenin. The amounts of co-precipitated nucleic acids were analyzed and binding levels upto 90 μg DNA/mg amelogenin was achieved. The co-precipitation could also be carried out in a bacterial cell homogenate, and no bacterial proteins were found in the amelogenin aggregates, suggesting specificity for nucleic acid binding. Dynamic light scattering showed that amelogenin nanosphere structure is maintained upon DNA binding with an upto 2.6 nm increase in diameter. The reported binding of nucleic acids to amelogenin can be explored practically for nucleic acid separation. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4334076
- author
- Svensson Bonde, Johan
LU
and Bülow, Leif
LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biotechnology Letters
- volume
- 36
- issue
- 6
- pages
- 1349 - 1357
- publisher
- Springer
- external identifiers
-
- pmid:24563322
- wos:000335145800029
- scopus:84899911653
- pmid:24563322
- ISSN
- 1573-6776
- DOI
- 10.1007/s10529-014-1492-7
- language
- English
- LU publication?
- yes
- id
- dcce0355-3048-4212-9b22-5a750b5f0489 (old id 4334076)
- date added to LUP
- 2016-04-01 10:50:49
- date last changed
- 2023-08-31 12:49:55
@article{dcce0355-3048-4212-9b22-5a750b5f0489,
abstract = {{Amelogenin, a matrix protein involved in biomineralization of enamel, can self-assemble to form nanospheres in a pH-dependent manner. Nucleic acids (single-stranded, double-stranded, and plasmid DNA, as well as RNA) could be co-precipitated with amelogenin, demonstrating a strong binding of nucleic acids to amelogenin. The amounts of co-precipitated nucleic acids were analyzed and binding levels upto 90 μg DNA/mg amelogenin was achieved. The co-precipitation could also be carried out in a bacterial cell homogenate, and no bacterial proteins were found in the amelogenin aggregates, suggesting specificity for nucleic acid binding. Dynamic light scattering showed that amelogenin nanosphere structure is maintained upon DNA binding with an upto 2.6 nm increase in diameter. The reported binding of nucleic acids to amelogenin can be explored practically for nucleic acid separation.}},
author = {{Svensson Bonde, Johan and Bülow, Leif}},
issn = {{1573-6776}},
language = {{eng}},
number = {{6}},
pages = {{1349--1357}},
publisher = {{Springer}},
series = {{Biotechnology Letters}},
title = {{In vitro preparation of amelogenin nanoparticles carrying nucleic acids.}},
url = {{http://dx.doi.org/10.1007/s10529-014-1492-7}},
doi = {{10.1007/s10529-014-1492-7}},
volume = {{36}},
year = {{2014}},
}