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Bone mineral crystal size and organization vary across mature rat bone cortex

Turunen, Mikael J. LU ; Dovling Kaspersen, Jorn LU ; Olsson, Ulf LU ; Guizar-Sicairos, Manuel; Bech, Martin LU ; Schaff, Florian; Tägil, Magnus LU ; Jurvelin, Jukka S. and Isaksson, Hanna LU (2016) In Journal of Structural Biology 195(3). p.337-344
Abstract

The macro- and micro-features of bone can be assessed by using imaging methods. However, nano- and molecular features require more detailed characterization, such as use of e.g., vibrational spectroscopy and X-ray scattering. Nano- and molecular features also affect the mechanical competence of bone tissue. The aim of the present study was to reveal the effects of mineralization and its alterations on the mineral crystal scale, by investigating the spatial variation of molecular composition and mineral crystal structure across the cross-section of femur diaphyses in young rats, and healthy and osteoporotic mature rats (N = 5). Fourier transform infrared spectroscopy and scanning small- and wide-angle X-ray scattering (SAXS/WAXS)... (More)

The macro- and micro-features of bone can be assessed by using imaging methods. However, nano- and molecular features require more detailed characterization, such as use of e.g., vibrational spectroscopy and X-ray scattering. Nano- and molecular features also affect the mechanical competence of bone tissue. The aim of the present study was to reveal the effects of mineralization and its alterations on the mineral crystal scale, by investigating the spatial variation of molecular composition and mineral crystal structure across the cross-section of femur diaphyses in young rats, and healthy and osteoporotic mature rats (N = 5). Fourier transform infrared spectroscopy and scanning small- and wide-angle X-ray scattering (SAXS/WAXS) techniques with high spatial resolution were used at identical locations over the whole cross-section. This allowed quantification of point-by-point information about the spatial distribution of mineral crystal volume. All measured parameters (crystal dimensions, degree of orientation and predominant orientation) varied across the cortex. Specifically, the crystal dimensions were lower in the central cortex than in the endosteal and periosteal regions. Mineral crystal orientation followed the cortical circumference in the periosteal and endosteal regions, but was less well-oriented in the central regions. Central cortex is formed rapidly during development through endochondral ossification. Since rats possess no osteonal remodeling, this bone remains (until old age). Significant linear correlations were observed between the dimensional and organizational parameters, e.g., between crystal length and degree of orientation (R2 = 0.83, p < 0.001). Application of SAXS/WAXS provides valuable information on bone nanostructure and its constituents, effects of diseases and, prospectively, mechanical competence.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bone mineralization, Fourier transform infrared spectroscopy, Osteoporosis, Rat, Small/wide angle X-ray scattering
in
Journal of Structural Biology
volume
195
issue
3
pages
8 pages
publisher
Elsevier
external identifiers
  • scopus:84991325300
  • wos:000381777700008
ISSN
1047-8477
DOI
10.1016/j.jsb.2016.07.005
language
English
LU publication?
yes
id
dd7d7f06-61b7-4c8c-885d-30aaf323b5ac
date added to LUP
2016-12-12 15:13:22
date last changed
2017-10-22 05:23:43
@article{dd7d7f06-61b7-4c8c-885d-30aaf323b5ac,
  abstract     = {<p>The macro- and micro-features of bone can be assessed by using imaging methods. However, nano- and molecular features require more detailed characterization, such as use of e.g., vibrational spectroscopy and X-ray scattering. Nano- and molecular features also affect the mechanical competence of bone tissue. The aim of the present study was to reveal the effects of mineralization and its alterations on the mineral crystal scale, by investigating the spatial variation of molecular composition and mineral crystal structure across the cross-section of femur diaphyses in young rats, and healthy and osteoporotic mature rats (N = 5). Fourier transform infrared spectroscopy and scanning small- and wide-angle X-ray scattering (SAXS/WAXS) techniques with high spatial resolution were used at identical locations over the whole cross-section. This allowed quantification of point-by-point information about the spatial distribution of mineral crystal volume. All measured parameters (crystal dimensions, degree of orientation and predominant orientation) varied across the cortex. Specifically, the crystal dimensions were lower in the central cortex than in the endosteal and periosteal regions. Mineral crystal orientation followed the cortical circumference in the periosteal and endosteal regions, but was less well-oriented in the central regions. Central cortex is formed rapidly during development through endochondral ossification. Since rats possess no osteonal remodeling, this bone remains (until old age). Significant linear correlations were observed between the dimensional and organizational parameters, e.g., between crystal length and degree of orientation (R<sup>2</sup> = 0.83, p &lt; 0.001). Application of SAXS/WAXS provides valuable information on bone nanostructure and its constituents, effects of diseases and, prospectively, mechanical competence.</p>},
  author       = {Turunen, Mikael J. and Dovling Kaspersen, Jorn and Olsson, Ulf and Guizar-Sicairos, Manuel and Bech, Martin and Schaff, Florian and Tägil, Magnus and Jurvelin, Jukka S. and Isaksson, Hanna},
  issn         = {1047-8477},
  keyword      = {Bone mineralization,Fourier transform infrared spectroscopy,Osteoporosis,Rat,Small/wide angle X-ray scattering},
  language     = {eng},
  month        = {09},
  number       = {3},
  pages        = {337--344},
  publisher    = {Elsevier},
  series       = {Journal of Structural Biology},
  title        = {Bone mineral crystal size and organization vary across mature rat bone cortex},
  url          = {http://dx.doi.org/10.1016/j.jsb.2016.07.005},
  volume       = {195},
  year         = {2016},
}