Influence of the reaction medium on the product distribution of peroxidase-catalysed oxidation of p-cresol
Pietikäinen, Pekka and Adlercreutz, Patrick LU
(1990)
In Applied Microbiology and Biotechnology
33(4).
p.455-458
- Abstract
p-Cresol was oxidized by hydrogen peroxide in a reaction catalysed by horseradish peroxidase and the low molecular weight products were investigated. In aqueous media Pummerer's ketone (I) was the dominating product but in organic media the product distribution was quite different; 2,2'-dihydroxy-5,5'-dimethyldiphenyl (II) was the main low molecular weight product. Similar product distributions were obtained with peroxidase adsorbed on a solid support and suspended in toluene and with peroxidase solubilized in a microemulsion containing the same solvent. The best selectivity for the formation of (II) was obtained when the enzyme was adsorbed on Celite and suspended in water-saturated chloroform with 0.5% (v/v) extra water added. The... (More)
p-Cresol was oxidized by hydrogen peroxide in a reaction catalysed by horseradish peroxidase and the low molecular weight products were investigated. In aqueous media Pummerer's ketone (I) was the dominating product but in organic media the product distribution was quite different; 2,2'-dihydroxy-5,5'-dimethyldiphenyl (II) was the main low molecular weight product. Similar product distributions were obtained with peroxidase adsorbed on a solid support and suspended in toluene and with peroxidase solubilized in a microemulsion containing the same solvent. The best selectivity for the formation of (II) was obtained when the enzyme was adsorbed on Celite and suspended in water-saturated chloroform with 0.5% (v/v) extra water added. The yield of low molecular weight products in this case was 28%; of this fraction, 95% was (II).
(Less)
- author
- Pietikäinen, Pekka
and Adlercreutz, Patrick
LU
- organization
- publishing date
- 1990-07-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Applied Microbiology and Biotechnology
- volume
- 33
- issue
- 4
- pages
- 4 pages
- publisher
- Springer
- external identifiers
-
- pmid:1366748
- scopus:0025073661
- ISSN
- 0175-7598
- DOI
- 10.1007/BF00176665
- language
- English
- LU publication?
- yes
- id
- ddad782f-3d0f-4005-8b41-f38270958d49
- date added to LUP
- 2019-06-22 18:55:50
- date last changed
- 2024-01-01 12:25:33
@article{ddad782f-3d0f-4005-8b41-f38270958d49,
abstract = {{<p>p-Cresol was oxidized by hydrogen peroxide in a reaction catalysed by horseradish peroxidase and the low molecular weight products were investigated. In aqueous media Pummerer's ketone (I) was the dominating product but in organic media the product distribution was quite different; 2,2'-dihydroxy-5,5'-dimethyldiphenyl (II) was the main low molecular weight product. Similar product distributions were obtained with peroxidase adsorbed on a solid support and suspended in toluene and with peroxidase solubilized in a microemulsion containing the same solvent. The best selectivity for the formation of (II) was obtained when the enzyme was adsorbed on Celite and suspended in water-saturated chloroform with 0.5% (v/v) extra water added. The yield of low molecular weight products in this case was 28%; of this fraction, 95% was (II).</p>}},
author = {{Pietikäinen, Pekka and Adlercreutz, Patrick}},
issn = {{0175-7598}},
language = {{eng}},
month = {{07}},
number = {{4}},
pages = {{455--458}},
publisher = {{Springer}},
series = {{Applied Microbiology and Biotechnology}},
title = {{Influence of the reaction medium on the product distribution of peroxidase-catalysed oxidation of p-cresol}},
url = {{http://dx.doi.org/10.1007/BF00176665}},
doi = {{10.1007/BF00176665}},
volume = {{33}},
year = {{1990}},
}