Real-time observation of nonclassical protein crystallization kinetics
(2015) In Journal of the American Chemical Society 137(4). p.1485-1491- Abstract
We present a real-time study of protein crystallization of bovine-lactoglobulin in the presence of CdCl2 using small-angle X-ray scattering and optical microscopy. From observing the crystallization kinetics, we propose the following multistep crystallization mechanism that is consistent with our data. In the first step, an intermediate phase is formed, followed by the nucleation of crystals within the intermediate phase. During this period, the number of crystals increases with time, but the crystal growth is slowed down by the surrounding dense intermediate phase due to the low mobility. In the next step, the intermediate phase is consumed by nucleation and slow growth, and the crystals are exposed to the dilute phase. In... (More)
We present a real-time study of protein crystallization of bovine-lactoglobulin in the presence of CdCl2 using small-angle X-ray scattering and optical microscopy. From observing the crystallization kinetics, we propose the following multistep crystallization mechanism that is consistent with our data. In the first step, an intermediate phase is formed, followed by the nucleation of crystals within the intermediate phase. During this period, the number of crystals increases with time, but the crystal growth is slowed down by the surrounding dense intermediate phase due to the low mobility. In the next step, the intermediate phase is consumed by nucleation and slow growth, and the crystals are exposed to the dilute phase. In this stage, the number of crystals becomes nearly constant, whereas the crystals grow rapidly due to access to the free protein molecules in the dilute phase. This real-time study not only provides evidence for a two-step nucleation process for protein crystallization but also elucidates the role and the structural signature of the metastable intermediate phase in this process.
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- author
- Sauter, Andrea ; Roosen-Runge, Felix LU ; Zhang, Fajun ; Lotze, Gudrun LU ; Jacobs, Robert M.J. and Schreiber, Frank
- publishing date
- 2015-02-04
- type
- Contribution to journal
- publication status
- published
- in
- Journal of the American Chemical Society
- volume
- 137
- issue
- 4
- pages
- 7 pages
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- pmid:25569484
- scopus:84922347272
- ISSN
- 0002-7863
- DOI
- 10.1021/ja510533x
- language
- English
- LU publication?
- no
- id
- de0bfa48-e69f-48cf-9c99-50e8392ad9a1
- date added to LUP
- 2018-12-17 09:43:30
- date last changed
- 2024-03-02 15:07:25
@article{de0bfa48-e69f-48cf-9c99-50e8392ad9a1,
abstract = {{<p>We present a real-time study of protein crystallization of bovine-lactoglobulin in the presence of CdCl<sub>2</sub> using small-angle X-ray scattering and optical microscopy. From observing the crystallization kinetics, we propose the following multistep crystallization mechanism that is consistent with our data. In the first step, an intermediate phase is formed, followed by the nucleation of crystals within the intermediate phase. During this period, the number of crystals increases with time, but the crystal growth is slowed down by the surrounding dense intermediate phase due to the low mobility. In the next step, the intermediate phase is consumed by nucleation and slow growth, and the crystals are exposed to the dilute phase. In this stage, the number of crystals becomes nearly constant, whereas the crystals grow rapidly due to access to the free protein molecules in the dilute phase. This real-time study not only provides evidence for a two-step nucleation process for protein crystallization but also elucidates the role and the structural signature of the metastable intermediate phase in this process.</p>}},
author = {{Sauter, Andrea and Roosen-Runge, Felix and Zhang, Fajun and Lotze, Gudrun and Jacobs, Robert M.J. and Schreiber, Frank}},
issn = {{0002-7863}},
language = {{eng}},
month = {{02}},
number = {{4}},
pages = {{1485--1491}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Journal of the American Chemical Society}},
title = {{Real-time observation of nonclassical protein crystallization kinetics}},
url = {{http://dx.doi.org/10.1021/ja510533x}},
doi = {{10.1021/ja510533x}},
volume = {{137}},
year = {{2015}},
}