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HPV16 E5 is produced from an HPV16 early mRNA spliced from SD226 to SA3358

Nilsson, Kersti LU ; Norberg, Christopher; Mossberg, Ann-Kristin LU and Schwartz, Stefan LU (2018) In Virus Research 244. p.128-136
Abstract

The HPV16 E5 open reading frame (ORF) is present on the majority of all alternatively spliced HPV16 mRNAs, but it is currently unknown how well it is translated into E5 protein. To identify HPV16 mRNAs that are efficiently translated into E5, we have generated cDNA plasmids expressing individual, alternatively spliced HPV16 mRNAs with the potential to produce E5. By replacing the E5 ORF with sLuc, we could quantitate sLuc and determine how well each cDNA was translated. Our results showed that the upstream E1 and E7 AUGs inhibited translation of the E5 ORF and revealed that only one HPV16 mRNA produced high levels of E5. This was an HPV16 early mRNA spliced from SD226 to SA3358. These results were confirmed in the context of the entire... (More)

The HPV16 E5 open reading frame (ORF) is present on the majority of all alternatively spliced HPV16 mRNAs, but it is currently unknown how well it is translated into E5 protein. To identify HPV16 mRNAs that are efficiently translated into E5, we have generated cDNA plasmids expressing individual, alternatively spliced HPV16 mRNAs with the potential to produce E5. By replacing the E5 ORF with sLuc, we could quantitate sLuc and determine how well each cDNA was translated. Our results showed that the upstream E1 and E7 AUGs inhibited translation of the E5 ORF and revealed that only one HPV16 mRNA produced high levels of E5. This was an HPV16 early mRNA spliced from SD226 to SA3358. These results were confirmed in the context of the entire HPV16 genome. Taken together, our results indicate that E5 is expressed early in the HPV16 replication cycle since it is translated efficiently only by one early mRNA.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
E5, Hpv, mRNA, Splicing, Translation
in
Virus Research
volume
244
pages
128 - 136
publisher
Elsevier
external identifiers
  • scopus:85034420391
ISSN
0168-1702
DOI
10.1016/j.virusres.2017.11.009
language
English
LU publication?
yes
id
de5b22be-8354-47d3-8c66-417f511ff0ab
date added to LUP
2017-12-21 09:34:00
date last changed
2018-01-19 12:58:15
@article{de5b22be-8354-47d3-8c66-417f511ff0ab,
  abstract     = {<p>The HPV16 E5 open reading frame (ORF) is present on the majority of all alternatively spliced HPV16 mRNAs, but it is currently unknown how well it is translated into E5 protein. To identify HPV16 mRNAs that are efficiently translated into E5, we have generated cDNA plasmids expressing individual, alternatively spliced HPV16 mRNAs with the potential to produce E5. By replacing the E5 ORF with sLuc, we could quantitate sLuc and determine how well each cDNA was translated. Our results showed that the upstream E1 and E7 AUGs inhibited translation of the E5 ORF and revealed that only one HPV16 mRNA produced high levels of E5. This was an HPV16 early mRNA spliced from SD226 to SA3358. These results were confirmed in the context of the entire HPV16 genome. Taken together, our results indicate that E5 is expressed early in the HPV16 replication cycle since it is translated efficiently only by one early mRNA.</p>},
  author       = {Nilsson, Kersti and Norberg, Christopher and Mossberg, Ann-Kristin and Schwartz, Stefan},
  issn         = {0168-1702},
  keyword      = {E5,Hpv,mRNA,Splicing,Translation},
  language     = {eng},
  pages        = {128--136},
  publisher    = {Elsevier},
  series       = {Virus Research},
  title        = {HPV16 E5 is produced from an HPV16 early mRNA spliced from SD226 to SA3358},
  url          = {http://dx.doi.org/10.1016/j.virusres.2017.11.009},
  volume       = {244},
  year         = {2018},
}