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Porous silicon microarray for simultaneous fluorometric immunoassay of the biomarkers prostate-specific antigen and human glandular kallikrein 2

Lee, Sang Wook; Hosokawa, Kazuo; Kim, Soyoun; Jeong, Ok Chan; Lilja, Hans LU ; Laurell, Thomas LU and Maeda, Mizuo (2016) In Mikrochimica Acta 183(12). p.3321-3327
Abstract

The authors have developed a porous silicon (P-Si) based duplex antibody microarray platform for simultaneous quantitation of the biomarkers prostate-specific antigen (PSA) and human glandular kallikrein 2 (hK2) in serum. Pore size-controlled P-Si surfaces have an extremely enlarged surface area that enables high-density immobilization of fluorescently labeled antibodies by physical adsorption. Automated microarraying of the antibodies provides a fast and reproducible duplex format of antibody arrays on the P-Si chips placed in the wells of a microtiter plate. The assay platform showed a 100 fg·mL−1 limit of detection for both PSA and hK2, and a dynamic range that extends over five orders of magnitude. After optimization of... (More)

The authors have developed a porous silicon (P-Si) based duplex antibody microarray platform for simultaneous quantitation of the biomarkers prostate-specific antigen (PSA) and human glandular kallikrein 2 (hK2) in serum. Pore size-controlled P-Si surfaces have an extremely enlarged surface area that enables high-density immobilization of fluorescently labeled antibodies by physical adsorption. Automated microarraying of the antibodies provides a fast and reproducible duplex format of antibody arrays on the P-Si chips placed in the wells of a microtiter plate. The assay platform showed a 100 fg·mL−1 limit of detection for both PSA and hK2, and a dynamic range that extends over five orders of magnitude. After optimization of the density of both capture antibodies, neither the PSA nor the hK2 array showed cross-sensitivity to non-target proteins or other plasma proteins. The microarray was evaluated by titration of PSA and hK2, respectively, in the same serum samples. In our perception, this highly sensitive and selective platform holds promise for improved detection of tumor markers in an early diagnostic stage, but also to monitor the recurrence of prostate cancer. [Figure not available: see fulltext.]

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Duplex antibody microarray, hK2, Prostate cancer, PSA, Serum analysis, Silicon anodization
in
Mikrochimica Acta
volume
183
issue
12
pages
7 pages
publisher
Springer
external identifiers
  • scopus:84991782479
  • wos:000389194500027
ISSN
0026-3672
DOI
10.1007/s00604-016-1986-1
language
English
LU publication?
yes
id
debd3813-c13a-4c6b-8fcd-6a296506cba8
date added to LUP
2016-11-09 09:38:55
date last changed
2017-10-29 04:53:50
@article{debd3813-c13a-4c6b-8fcd-6a296506cba8,
  abstract     = {<p>The authors have developed a porous silicon (P-Si) based duplex antibody microarray platform for simultaneous quantitation of the biomarkers prostate-specific antigen (PSA) and human glandular kallikrein 2 (hK2) in serum. Pore size-controlled P-Si surfaces have an extremely enlarged surface area that enables high-density immobilization of fluorescently labeled antibodies by physical adsorption. Automated microarraying of the antibodies provides a fast and reproducible duplex format of antibody arrays on the P-Si chips placed in the wells of a microtiter plate. The assay platform showed a 100 fg·mL<sup>−1</sup> limit of detection for both PSA and hK2, and a dynamic range that extends over five orders of magnitude. After optimization of the density of both capture antibodies, neither the PSA nor the hK2 array showed cross-sensitivity to non-target proteins or other plasma proteins. The microarray was evaluated by titration of PSA and hK2, respectively, in the same serum samples. In our perception, this highly sensitive and selective platform holds promise for improved detection of tumor markers in an early diagnostic stage, but also to monitor the recurrence of prostate cancer. [Figure not available: see fulltext.]</p>},
  author       = {Lee, Sang Wook and Hosokawa, Kazuo and Kim, Soyoun and Jeong, Ok Chan and Lilja, Hans and Laurell, Thomas and Maeda, Mizuo},
  issn         = {0026-3672},
  keyword      = {Duplex antibody microarray,hK2,Prostate cancer,PSA,Serum analysis,Silicon anodization},
  language     = {eng},
  number       = {12},
  pages        = {3321--3327},
  publisher    = {Springer},
  series       = {Mikrochimica Acta},
  title        = {Porous silicon microarray for simultaneous fluorometric immunoassay of the biomarkers prostate-specific antigen and human glandular kallikrein 2},
  url          = {http://dx.doi.org/10.1007/s00604-016-1986-1},
  volume       = {183},
  year         = {2016},
}