Synthesis of 2-acetamido-2-deoxy-3-O-β-d-galactopyrano-syl-d-galactose by the sequential use of β-d-galactosidases from bovine testes and escherichia coli
(1989) In Carbohydrate Research 186(2). p.217-223- Abstract
- β-d-Galp-(1→3)-d-GalNAc (1) was synthesised from lactose and GalNAc on a mmolar scale by transgalactosylation using β-d-galactosidase from bovine testes. The large proportions of unwanted oligosaccharides in the product mixture were removed by treatment with β-d-galactosidase from E. coli, which left 1, monosaccharides, and a small proportion of trisaccharides. Carbon-Celite chromatography then gave 1 in a yield of 21% based on the GalNAc added.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/e08417f1-7c0a-492e-86b0-c0a778fe4d32
- author
- Hedbys, Lars ; Johansson, Elisabet ; Mosbach, Klaus LU ; Larsson, Per-Olof LU ; Gunnarsson, Alf and Svensson, Sigfrid
- organization
- publishing date
- 1989
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- β-D-Galp-(1→3)-D-GalNAc, Transgalactosylation, β-galactosidase, E. coli, Bovine testes
- in
- Carbohydrate Research
- volume
- 186
- issue
- 2
- pages
- 7 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0024969887
- ISSN
- 0008-6215
- DOI
- 10.1016/0008-6215(89)84036-4
- language
- English
- LU publication?
- yes
- id
- e08417f1-7c0a-492e-86b0-c0a778fe4d32
- date added to LUP
- 2024-06-17 14:53:16
- date last changed
- 2024-09-02 15:16:23
@article{e08417f1-7c0a-492e-86b0-c0a778fe4d32, abstract = {{β-d-Galp-(1→3)-d-GalNAc (1) was synthesised from lactose and GalNAc on a mmolar scale by transgalactosylation using β-d-galactosidase from bovine testes. The large proportions of unwanted oligosaccharides in the product mixture were removed by treatment with β-d-galactosidase from E. coli, which left 1, monosaccharides, and a small proportion of trisaccharides. Carbon-Celite chromatography then gave 1 in a yield of 21% based on the GalNAc added.}}, author = {{Hedbys, Lars and Johansson, Elisabet and Mosbach, Klaus and Larsson, Per-Olof and Gunnarsson, Alf and Svensson, Sigfrid}}, issn = {{0008-6215}}, keywords = {{β-D-Galp-(1→3)-D-GalNAc; Transgalactosylation; β-galactosidase; E. coli; Bovine testes}}, language = {{eng}}, number = {{2}}, pages = {{217--223}}, publisher = {{Elsevier}}, series = {{Carbohydrate Research}}, title = {{Synthesis of 2-acetamido-2-deoxy-3-O-β-d-galactopyrano-syl-d-galactose by the sequential use of β-d-galactosidases from bovine testes and <i>escherichia coli</i>}}, url = {{http://dx.doi.org/10.1016/0008-6215(89)84036-4}}, doi = {{10.1016/0008-6215(89)84036-4}}, volume = {{186}}, year = {{1989}}, }