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Comprehensive on-line two-dimensional liquid chromatography × supercritical fluid chromatography with trapping column-assisted modulation for depolymerised lignin analysis

Sun, Mingzhe LU ; Sandahl, Margareta LU and Turner, Charlotta LU (2018) In Journal of Chromatography A
Abstract

Lignin depolymerisation produces a large variety of low molecular weight phenolic compounds that can be upgraded to value-added chemicals. Detailed analysis of these complex depolymerisation mixtures is, however, hampered by the lack of resolving power of traditional analysis techniques. In this study, a novel online comprehensive two-dimensional reversed-phase liquid chromatography (RPLC) × supercritical fluid chromatography (SFC) method with trapping column interface was developed for the separation of phenolic compounds in depolymerised lignin samples. The trapping capacities of different trapping columns were evaluated. The influence of large volume water-containing injection on SFC performance was studied. The relation between peak... (More)

Lignin depolymerisation produces a large variety of low molecular weight phenolic compounds that can be upgraded to value-added chemicals. Detailed analysis of these complex depolymerisation mixtures is, however, hampered by the lack of resolving power of traditional analysis techniques. In this study, a novel online comprehensive two-dimensional reversed-phase liquid chromatography (RPLC) × supercritical fluid chromatography (SFC) method with trapping column interface was developed for the separation of phenolic compounds in depolymerised lignin samples. The trapping capacities of different trapping columns were evaluated. The influence of large volume water-containing injection on SFC performance was studied. The relation between peak capacity and first dimension flow rate and gradient was investigated. The optimized method was applied for the analysis of a depolymerised lignin sample. The RPLC × SFC system exhibited high degree of orthogonality. Compared with traditional loop based interface, trapping column interface can significantly shorten the analysis time and offer higher detectability, with the disadvantage of more severe undersampling in the first dimension.

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author
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
Lignin, Phenolic compound, Supercritical fluid chromatography, Trapping capacity, Two-dimensional chromatography
in
Journal of Chromatography A
publisher
Elsevier
external identifiers
  • scopus:85041957289
ISSN
0021-9673
DOI
10.1016/j.chroma.2018.02.008
language
English
LU publication?
yes
id
e0aea681-dd57-4ef8-9609-e8e08a7fa286
date added to LUP
2018-02-21 14:35:28
date last changed
2018-07-15 04:47:37
@article{e0aea681-dd57-4ef8-9609-e8e08a7fa286,
  abstract     = {<p>Lignin depolymerisation produces a large variety of low molecular weight phenolic compounds that can be upgraded to value-added chemicals. Detailed analysis of these complex depolymerisation mixtures is, however, hampered by the lack of resolving power of traditional analysis techniques. In this study, a novel online comprehensive two-dimensional reversed-phase liquid chromatography (RPLC) × supercritical fluid chromatography (SFC) method with trapping column interface was developed for the separation of phenolic compounds in depolymerised lignin samples. The trapping capacities of different trapping columns were evaluated. The influence of large volume water-containing injection on SFC performance was studied. The relation between peak capacity and first dimension flow rate and gradient was investigated. The optimized method was applied for the analysis of a depolymerised lignin sample. The RPLC × SFC system exhibited high degree of orthogonality. Compared with traditional loop based interface, trapping column interface can significantly shorten the analysis time and offer higher detectability, with the disadvantage of more severe undersampling in the first dimension.</p>},
  author       = {Sun, Mingzhe and Sandahl, Margareta and Turner, Charlotta},
  issn         = {0021-9673},
  keyword      = {Lignin,Phenolic compound,Supercritical fluid chromatography,Trapping capacity,Two-dimensional chromatography},
  language     = {eng},
  month        = {02},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {Comprehensive on-line two-dimensional liquid chromatography × supercritical fluid chromatography with trapping column-assisted modulation for depolymerised lignin analysis},
  url          = {http://dx.doi.org/10.1016/j.chroma.2018.02.008},
  year         = {2018},
}