Comprehensive on-line two-dimensional liquid chromatography × supercritical fluid chromatography with trapping column-assisted modulation for depolymerised lignin analysis
Sun, Mingzhe LU ; Sandahl, Margareta LU and Turner, Charlotta LU
(2018)
In Journal of Chromatography A
1541.
p.21-30
- Abstract
Lignin depolymerisation produces a large variety of low molecular weight phenolic compounds that can be upgraded to value-added chemicals. Detailed analysis of these complex depolymerisation mixtures is, however, hampered by the lack of resolving power of traditional analysis techniques. In this study, a novel online comprehensive two-dimensional reversed-phase liquid chromatography (RPLC) × supercritical fluid chromatography (SFC) method with trapping column interface was developed for the separation of phenolic compounds in depolymerised lignin samples. The trapping capacities of different trapping columns were evaluated. The influence of large volume water-containing injection on SFC performance was studied. The relation between peak... (More)
Lignin depolymerisation produces a large variety of low molecular weight phenolic compounds that can be upgraded to value-added chemicals. Detailed analysis of these complex depolymerisation mixtures is, however, hampered by the lack of resolving power of traditional analysis techniques. In this study, a novel online comprehensive two-dimensional reversed-phase liquid chromatography (RPLC) × supercritical fluid chromatography (SFC) method with trapping column interface was developed for the separation of phenolic compounds in depolymerised lignin samples. The trapping capacities of different trapping columns were evaluated. The influence of large volume water-containing injection on SFC performance was studied. The relation between peak capacity and first dimension flow rate and gradient was investigated. The optimized method was applied for the analysis of a depolymerised lignin sample. The RPLC × SFC system exhibited high degree of orthogonality. Compared with traditional loop based interface, trapping column interface can significantly shorten the analysis time and offer higher detectability, with the disadvantage of more severe undersampling in the first dimension.
(Less)
- author
- Sun, Mingzhe
LU
; Sandahl, Margareta
LU
and Turner, Charlotta
LU
- organization
- publishing date
- 2018-02-06
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Lignin, Phenolic compound, Supercritical fluid chromatography, Trapping capacity, Two-dimensional chromatography
- in
- Journal of Chromatography A
- volume
- 1541
- pages
- 21 - 30
- publisher
- Elsevier
- external identifiers
-
- scopus:85041957289
- pmid:29452928
- ISSN
- 0021-9673
- DOI
- 10.1016/j.chroma.2018.02.008
- language
- English
- LU publication?
- yes
- id
- e0aea681-dd57-4ef8-9609-e8e08a7fa286
- date added to LUP
- 2018-02-21 14:35:28
- date last changed
- 2024-07-08 09:45:12
@article{e0aea681-dd57-4ef8-9609-e8e08a7fa286,
abstract = {{<p>Lignin depolymerisation produces a large variety of low molecular weight phenolic compounds that can be upgraded to value-added chemicals. Detailed analysis of these complex depolymerisation mixtures is, however, hampered by the lack of resolving power of traditional analysis techniques. In this study, a novel online comprehensive two-dimensional reversed-phase liquid chromatography (RPLC) × supercritical fluid chromatography (SFC) method with trapping column interface was developed for the separation of phenolic compounds in depolymerised lignin samples. The trapping capacities of different trapping columns were evaluated. The influence of large volume water-containing injection on SFC performance was studied. The relation between peak capacity and first dimension flow rate and gradient was investigated. The optimized method was applied for the analysis of a depolymerised lignin sample. The RPLC × SFC system exhibited high degree of orthogonality. Compared with traditional loop based interface, trapping column interface can significantly shorten the analysis time and offer higher detectability, with the disadvantage of more severe undersampling in the first dimension.</p>}},
author = {{Sun, Mingzhe and Sandahl, Margareta and Turner, Charlotta}},
issn = {{0021-9673}},
keywords = {{Lignin; Phenolic compound; Supercritical fluid chromatography; Trapping capacity; Two-dimensional chromatography}},
language = {{eng}},
month = {{02}},
pages = {{21--30}},
publisher = {{Elsevier}},
series = {{Journal of Chromatography A}},
title = {{Comprehensive on-line two-dimensional liquid chromatography × supercritical fluid chromatography with trapping column-assisted modulation for depolymerised lignin analysis}},
url = {{http://dx.doi.org/10.1016/j.chroma.2018.02.008}},
doi = {{10.1016/j.chroma.2018.02.008}},
volume = {{1541}},
year = {{2018}},
}