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Time‐dependent effects of insulin on Schwann cell proliferation in the in vitro regenerating adult frog sciatic nerve

Ekström, P. A R ; Edbladh, M. LU and Edström, A. LU (1993) In Journal of Neuroscience Research 34(6). p.614-621
Abstract

The present study showed that insulin (0.01 μg/ml, ≈︂ 2 nM) inhibited [3H]‐thymidine incorporation in support cells, most likely Schwann cells, of the cultured frog sciatic nerve. A 25–35% inhibition took place in regenerating nerve preparations as well as in preparations devoid of neuronal protein synthesis, i.e., in isolated 5 mm nerve segments and in gangliectomized nerves, suggesting that the effect was direct and not mediated via the neuronal cells. The inhibition by insulin was time‐dependent in that an effect was seen after 4 days but not at shorter or at longer periods of culturing. In separate experiments biotinylated insulin was shown to be taken up by Schwann cells in the regenerating nerve. Addition of serum... (More)

The present study showed that insulin (0.01 μg/ml, ≈︂ 2 nM) inhibited [3H]‐thymidine incorporation in support cells, most likely Schwann cells, of the cultured frog sciatic nerve. A 25–35% inhibition took place in regenerating nerve preparations as well as in preparations devoid of neuronal protein synthesis, i.e., in isolated 5 mm nerve segments and in gangliectomized nerves, suggesting that the effect was direct and not mediated via the neuronal cells. The inhibition by insulin was time‐dependent in that an effect was seen after 4 days but not at shorter or at longer periods of culturing. In separate experiments biotinylated insulin was shown to be taken up by Schwann cells in the regenerating nerve. Addition of serum increased the [3H]‐thymidine incorporation severalfold and abolished the inhibitory action of insulin. Our results suggest that insulin, at a certain stage of the regeneration programme, exerts a direct, inhibitory effect on the proliferation of the Schwann cells in the cultured frog sciatic nerve. © 1993 Wiley‐Liss, Inc.

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publishing date
type
Contribution to journal
publication status
published
subject
keywords
inhibition, nerve regeneration, support cells
in
Journal of Neuroscience Research
volume
34
issue
6
pages
8 pages
publisher
John Wiley & Sons Inc.
external identifiers
  • pmid:8315661
  • scopus:0027241435
ISSN
0360-4012
DOI
10.1002/jnr.490340603
language
English
LU publication?
yes
id
e0bdebe8-5b45-484b-84b2-ef2ce17cfde7
date added to LUP
2016-12-07 14:39:24
date last changed
2025-01-12 17:07:11
@article{e0bdebe8-5b45-484b-84b2-ef2ce17cfde7,
  abstract     = {{<p>The present study showed that insulin (0.01 μg/ml, ≈︂ 2 nM) inhibited [<sup>3</sup>H]‐thymidine incorporation in support cells, most likely Schwann cells, of the cultured frog sciatic nerve. A 25–35% inhibition took place in regenerating nerve preparations as well as in preparations devoid of neuronal protein synthesis, i.e., in isolated 5 mm nerve segments and in gangliectomized nerves, suggesting that the effect was direct and not mediated via the neuronal cells. The inhibition by insulin was time‐dependent in that an effect was seen after 4 days but not at shorter or at longer periods of culturing. In separate experiments biotinylated insulin was shown to be taken up by Schwann cells in the regenerating nerve. Addition of serum increased the [<sup>3</sup>H]‐thymidine incorporation severalfold and abolished the inhibitory action of insulin. Our results suggest that insulin, at a certain stage of the regeneration programme, exerts a direct, inhibitory effect on the proliferation of the Schwann cells in the cultured frog sciatic nerve. © 1993 Wiley‐Liss, Inc.</p>}},
  author       = {{Ekström, P. A R and Edbladh, M. and Edström, A.}},
  issn         = {{0360-4012}},
  keywords     = {{inhibition; nerve regeneration; support cells}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{614--621}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Journal of Neuroscience Research}},
  title        = {{Time‐dependent effects of insulin on Schwann cell proliferation in the in vitro regenerating adult frog sciatic nerve}},
  url          = {{http://dx.doi.org/10.1002/jnr.490340603}},
  doi          = {{10.1002/jnr.490340603}},
  volume       = {{34}},
  year         = {{1993}},
}