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Approaches to molecular imprinting based selectivity in capillary electrochromatography.

Schweitz, Leif LU ; Spégel, Peter LU and Nilsson, Staffan LU (2001) In Electrophoresis 22(19). p.63-4053
Abstract
The work done during the past decade in order to adapt molecularly imprinted polymers (MIPs) to the capillary format and subsequently use these highly selective matrices for capillary electrochromatography (CEC) are reviewed in this article. MIPs are prepared utilizing a templated polymer synthesis where the template addresses the selectivity of the resulting polymer. These polymers possess binding characteristics that are comparable to the biological antibodies. Due to the polyclonality of the binding sites in the MIP, the separation result in severe peak broadening and tailing when performed in the isocratic mode. This was seen early in the development of MIPs as selective stationary phases in liquid chromatography (LC). As a mean of... (More)
The work done during the past decade in order to adapt molecularly imprinted polymers (MIPs) to the capillary format and subsequently use these highly selective matrices for capillary electrochromatography (CEC) are reviewed in this article. MIPs are prepared utilizing a templated polymer synthesis where the template addresses the selectivity of the resulting polymer. These polymers possess binding characteristics that are comparable to the biological antibodies. Due to the polyclonality of the binding sites in the MIP, the separation result in severe peak broadening and tailing when performed in the isocratic mode. This was seen early in the development of MIPs as selective stationary phases in liquid chromatography (LC). As a mean of decreasing these problems, much effort was put into adapting the MIP to fit in CEC systems, that offers an efficiency that is superior to that in LC. Aiming to increase the efficiency of the MIP-CEC systems, different MIP formats have been developed that can be divided into three conceptually different categories, i.e., the monolithic, the microparticle and the coating. The strive for MIP formats that can be used in small bore capillaries has led to the development of MIP formats applicable to miniaturized systems approaching the chip format. Although prepared in order to perform MIP-CEC mediated separations, these formats can be used in a broad range of applications were the characteristics of the MIP, e.g. stability, selectivity and cost efficiency, could offer an interesting solution to cover the needs. (Less)
Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Chromatography, Micellar Electrokinetic Capillary : methods, Microscopy, Electron, Scanning
in
Electrophoresis
volume
22
issue
19
pages
63 - 4053
publisher
John Wiley & Sons Inc.
external identifiers
  • scopus:0035210948
ISSN
0173-0835
DOI
10.1002/1522-2683(200111)22:19<4053::AID-ELPS4053>3.0.CO;2-H
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
id
e0e48eed-a531-4a86-a869-46531f96c918 (old id 107116)
date added to LUP
2016-04-01 11:55:29
date last changed
2022-03-28 17:44:39
@article{e0e48eed-a531-4a86-a869-46531f96c918,
  abstract     = {{The work done during the past decade in order to adapt molecularly imprinted polymers (MIPs) to the capillary format and subsequently use these highly selective matrices for capillary electrochromatography (CEC) are reviewed in this article. MIPs are prepared utilizing a templated polymer synthesis where the template addresses the selectivity of the resulting polymer. These polymers possess binding characteristics that are comparable to the biological antibodies. Due to the polyclonality of the binding sites in the MIP, the separation result in severe peak broadening and tailing when performed in the isocratic mode. This was seen early in the development of MIPs as selective stationary phases in liquid chromatography (LC). As a mean of decreasing these problems, much effort was put into adapting the MIP to fit in CEC systems, that offers an efficiency that is superior to that in LC. Aiming to increase the efficiency of the MIP-CEC systems, different MIP formats have been developed that can be divided into three conceptually different categories, i.e., the monolithic, the microparticle and the coating. The strive for MIP formats that can be used in small bore capillaries has led to the development of MIP formats applicable to miniaturized systems approaching the chip format. Although prepared in order to perform MIP-CEC mediated separations, these formats can be used in a broad range of applications were the characteristics of the MIP, e.g. stability, selectivity and cost efficiency, could offer an interesting solution to cover the needs.}},
  author       = {{Schweitz, Leif and Spégel, Peter and Nilsson, Staffan}},
  issn         = {{0173-0835}},
  keywords     = {{Chromatography; Micellar Electrokinetic Capillary : methods; Microscopy; Electron; Scanning}},
  language     = {{eng}},
  number       = {{19}},
  pages        = {{63--4053}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Electrophoresis}},
  title        = {{Approaches to molecular imprinting based selectivity in capillary electrochromatography.}},
  url          = {{http://dx.doi.org/10.1002/1522-2683(200111)22:19<4053::AID-ELPS4053>3.0.CO;2-H}},
  doi          = {{10.1002/1522-2683(200111)22:19<4053::AID-ELPS4053>3.0.CO;2-H}},
  volume       = {{22}},
  year         = {{2001}},
}