Control of an affinity purification procedure using a thermal biosensor
(1990) In Biotechnology and Bioengineering 36(7). p.723-726- Abstract
- Lactate dehydrogenase (LDH) was recovered from a solution by affinity binding to an N6-(6-aminohexyl)-AMP-Sepharose gel. An enzyme thermistor unit was employed to continously measure the activity of the unbound LDH. The enzyme activity signal from the enzyme thermistor was used in a PID controller to regulate the addition of AMP-Sepharose gel to the LDH solution. In another type of experiment, a desktop computer was utilized to control the addition of the adsorbent. Both systems worked satisfactorily, and enabled a rapid and accurate assessment of correct addition of adsorbent.
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- author
- Flygare, Lilian ; Larsson, Per-Olof LU and Danielsson, Bengt LU
- organization
- publishing date
- 1990
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Enzyme thermistor, Affinity purification
- in
- Biotechnology and Bioengineering
- volume
- 36
- issue
- 7
- pages
- 4 pages
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:0025701091
- ISSN
- 1097-0290
- DOI
- 10.1002/bit.260360710
- language
- English
- LU publication?
- yes
- id
- e1bd07e7-a19f-4c7d-8aee-e47ff330f7ca
- date added to LUP
- 2024-06-17 13:47:11
- date last changed
- 2024-08-29 11:51:34
@article{e1bd07e7-a19f-4c7d-8aee-e47ff330f7ca, abstract = {{Lactate dehydrogenase (LDH) was recovered from a solution by affinity binding to an N6-(6-aminohexyl)-AMP-Sepharose gel. An enzyme thermistor unit was employed to continously measure the activity of the unbound LDH. The enzyme activity signal from the enzyme thermistor was used in a PID controller to regulate the addition of AMP-Sepharose gel to the LDH solution. In another type of experiment, a desktop computer was utilized to control the addition of the adsorbent. Both systems worked satisfactorily, and enabled a rapid and accurate assessment of correct addition of adsorbent.}}, author = {{Flygare, Lilian and Larsson, Per-Olof and Danielsson, Bengt}}, issn = {{1097-0290}}, keywords = {{Enzyme thermistor; Affinity purification}}, language = {{eng}}, number = {{7}}, pages = {{723--726}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Biotechnology and Bioengineering}}, title = {{Control of an affinity purification procedure using a thermal biosensor}}, url = {{http://dx.doi.org/10.1002/bit.260360710}}, doi = {{10.1002/bit.260360710}}, volume = {{36}}, year = {{1990}}, }