Gene expression profiling of placentae from women with early- and late-onset pre-eclampsia: down-regulation of the angiogenesis-related genes ACVRL1 and EGFL7 in early-onset disease
(2012) In Molecular Human Reproduction 18(3). p.146-155- Abstract
- The underlying mechanisms behind the obstetric condition pre-eclampsia (PE) are still unclear. Manifestation of PE is heterogeneous and it has therefore been proposed to be a syndrome with different causes rather than one disease with a specific aetiology. Recently, we showed differences in circulating angiogenic factors between two subgroupsearly- and late-onset PE. To further elucidate the differences between the two, we investigated placental gene expression profiles. Whole genome microarray technology and bioinformatic analysis were used to evaluate gene expression profiles in placentae from early- (2432 gestational weeks, n 8) and late-onset (3641 gestational weeks, n 7) PE. The results were verified by using quantitative real-time... (More)
- The underlying mechanisms behind the obstetric condition pre-eclampsia (PE) are still unclear. Manifestation of PE is heterogeneous and it has therefore been proposed to be a syndrome with different causes rather than one disease with a specific aetiology. Recently, we showed differences in circulating angiogenic factors between two subgroupsearly- and late-onset PE. To further elucidate the differences between the two, we investigated placental gene expression profiles. Whole genome microarray technology and bioinformatic analysis were used to evaluate gene expression profiles in placentae from early- (2432 gestational weeks, n 8) and late-onset (3641 gestational weeks, n 7) PE. The results were verified by using quantitative real-time (qRT)PCR. We found significant differences in the expression of 196 genes in early- compared with late-onset PE, 45 of these genes showing a fold change above 2. Bioinformatic analysis revealed alterations in angiogenesis and regulation of cell motility. Two angiogenesis-associated transcripts (Egfl7 and Acvrl1) showed lower expression in early-onset PE versus late-onset PE (P 0.037 and P 0.003) and versus gestational age-matched controls (P 0.007 and P 0.011). We conclude that angiogenesis-associated genes are regulated in a different manner in the two subgroups, and that the gene expression profiles of early- and late-onset PE diverge, supporting the hypothesis of early- and late-onset PE being at least partly two separate entities. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2390930
- author
- Junus, K. ; Centlow, Magnus LU ; Wikstrom, A-K ; Larsson, Irene LU ; Hansson, Stefan LU and Olovsson, M.
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- angiogenesis, early-onset pre-eclampsia, gene expression, microarray, placenta
- in
- Molecular Human Reproduction
- volume
- 18
- issue
- 3
- pages
- 146 - 155
- publisher
- Oxford University Press
- external identifiers
-
- wos:000300726400005
- scopus:84857517106
- ISSN
- 1460-2407
- DOI
- 10.1093/molehr/gar067
- language
- English
- LU publication?
- yes
- id
- e2429921-2c80-4c72-b619-ee33a5065351 (old id 2390930)
- date added to LUP
- 2016-04-01 10:43:10
- date last changed
- 2022-04-04 20:44:30
@article{e2429921-2c80-4c72-b619-ee33a5065351, abstract = {{The underlying mechanisms behind the obstetric condition pre-eclampsia (PE) are still unclear. Manifestation of PE is heterogeneous and it has therefore been proposed to be a syndrome with different causes rather than one disease with a specific aetiology. Recently, we showed differences in circulating angiogenic factors between two subgroupsearly- and late-onset PE. To further elucidate the differences between the two, we investigated placental gene expression profiles. Whole genome microarray technology and bioinformatic analysis were used to evaluate gene expression profiles in placentae from early- (2432 gestational weeks, n 8) and late-onset (3641 gestational weeks, n 7) PE. The results were verified by using quantitative real-time (qRT)PCR. We found significant differences in the expression of 196 genes in early- compared with late-onset PE, 45 of these genes showing a fold change above 2. Bioinformatic analysis revealed alterations in angiogenesis and regulation of cell motility. Two angiogenesis-associated transcripts (Egfl7 and Acvrl1) showed lower expression in early-onset PE versus late-onset PE (P 0.037 and P 0.003) and versus gestational age-matched controls (P 0.007 and P 0.011). We conclude that angiogenesis-associated genes are regulated in a different manner in the two subgroups, and that the gene expression profiles of early- and late-onset PE diverge, supporting the hypothesis of early- and late-onset PE being at least partly two separate entities.}}, author = {{Junus, K. and Centlow, Magnus and Wikstrom, A-K and Larsson, Irene and Hansson, Stefan and Olovsson, M.}}, issn = {{1460-2407}}, keywords = {{angiogenesis; early-onset pre-eclampsia; gene expression; microarray; placenta}}, language = {{eng}}, number = {{3}}, pages = {{146--155}}, publisher = {{Oxford University Press}}, series = {{Molecular Human Reproduction}}, title = {{Gene expression profiling of placentae from women with early- and late-onset pre-eclampsia: down-regulation of the angiogenesis-related genes ACVRL1 and EGFL7 in early-onset disease}}, url = {{http://dx.doi.org/10.1093/molehr/gar067}}, doi = {{10.1093/molehr/gar067}}, volume = {{18}}, year = {{2012}}, }