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Protease activity of Blastocystis hominis

Sio, Selena W S ; Puthia, Manoj K LU ; Lee, Alex S Y ; Lu, Jia and Tan, Kevin S W (2006) In Parasitology Research 99(2). p.30-126
Abstract

Parasite-derived proteases are important for the parasite life cycle and the pathogenesis of the disease they produce. Proteases of intestinal protozoan parasite Blastocystis hominis were studied for the first time with azocasein assays and gelatin SDS-PAGE analysis. Parasitic lysates were found to have high protease activity and nine protease bands of low (20-33 kDa) and high (44-75 kDa) molecular weights were reported. Proteases were found to be pH-dependent and highest proteolytic activity was observed at neutral pH. Inhibition studies showed that B. hominis isolate B, like many other protozoan parasites, contains mainly cysteine proteases.

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author
; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Animals, Blastocystis hominis/drug effects, Cysteine Endopeptidases/metabolism, Electrophoresis, Polyacrylamide Gel, Humans, Peptide Hydrolases/drug effects, Protease Inhibitors/pharmacology
in
Parasitology Research
volume
99
issue
2
pages
30 - 126
publisher
Springer
external identifiers
  • scopus:33745044249
  • pmid:16518611
ISSN
0932-0113
DOI
10.1007/s00436-006-0131-1
language
English
LU publication?
no
id
e33325a2-3fe7-4858-80a6-9c0c4b270dc5
date added to LUP
2018-08-27 13:44:20
date last changed
2024-08-05 21:19:37
@article{e33325a2-3fe7-4858-80a6-9c0c4b270dc5,
  abstract     = {{<p>Parasite-derived proteases are important for the parasite life cycle and the pathogenesis of the disease they produce. Proteases of intestinal protozoan parasite Blastocystis hominis were studied for the first time with azocasein assays and gelatin SDS-PAGE analysis. Parasitic lysates were found to have high protease activity and nine protease bands of low (20-33 kDa) and high (44-75 kDa) molecular weights were reported. Proteases were found to be pH-dependent and highest proteolytic activity was observed at neutral pH. Inhibition studies showed that B. hominis isolate B, like many other protozoan parasites, contains mainly cysteine proteases.</p>}},
  author       = {{Sio, Selena W S and Puthia, Manoj K and Lee, Alex S Y and Lu, Jia and Tan, Kevin S W}},
  issn         = {{0932-0113}},
  keywords     = {{Animals; Blastocystis hominis/drug effects; Cysteine Endopeptidases/metabolism; Electrophoresis, Polyacrylamide Gel; Humans; Peptide Hydrolases/drug effects; Protease Inhibitors/pharmacology}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{30--126}},
  publisher    = {{Springer}},
  series       = {{Parasitology Research}},
  title        = {{Protease activity of Blastocystis hominis}},
  url          = {{http://dx.doi.org/10.1007/s00436-006-0131-1}},
  doi          = {{10.1007/s00436-006-0131-1}},
  volume       = {{99}},
  year         = {{2006}},
}