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Advances in proteomic workflows for systems biology

Malmström, Johan LU ; Lee, Hookeun and Aebersold, Ruedi (2007) In Current Opinion in Biotechnology 18(4). p.84-378
Abstract

Mass spectrometry, specifically the analysis of complex peptide mixtures by liquid chromatography and tandem mass spectrometry (shotgun proteomics) has been at the centre of proteomics research for the past decade. To overcome some of the fundamental limitations of the approach, including its limited sensitivity and high degree of redundancy, new proteomic workflows are being developed. Among these, targeting methods in which specific peptides are selectively isolated, identified and quantified are particularly promising. Here we summarize recent incremental advances in shotgun proteomic methods and outline emerging targeted workflows. The development of the target-driven approaches with their ability to detect and quantify identical,... (More)

Mass spectrometry, specifically the analysis of complex peptide mixtures by liquid chromatography and tandem mass spectrometry (shotgun proteomics) has been at the centre of proteomics research for the past decade. To overcome some of the fundamental limitations of the approach, including its limited sensitivity and high degree of redundancy, new proteomic workflows are being developed. Among these, targeting methods in which specific peptides are selectively isolated, identified and quantified are particularly promising. Here we summarize recent incremental advances in shotgun proteomic methods and outline emerging targeted workflows. The development of the target-driven approaches with their ability to detect and quantify identical, non-redundant sets of proteins in multiple repeat analyses will be crucially important for the application of proteomics to biomarker discovery and validation, and to systems biology research.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Mass Spectrometry, Models, Theoretical, Proteome, Proteomics, Systems Biology, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't
in
Current Opinion in Biotechnology
volume
18
issue
4
pages
7 pages
publisher
Elsevier
external identifiers
  • scopus:34548522775
ISSN
0958-1669
DOI
10.1016/j.copbio.2007.07.005
language
English
LU publication?
no
id
e46a8b6f-7eeb-420e-b3db-7ca077da748d
date added to LUP
2017-09-04 17:22:26
date last changed
2017-09-10 05:23:43
@article{e46a8b6f-7eeb-420e-b3db-7ca077da748d,
  abstract     = {<p>Mass spectrometry, specifically the analysis of complex peptide mixtures by liquid chromatography and tandem mass spectrometry (shotgun proteomics) has been at the centre of proteomics research for the past decade. To overcome some of the fundamental limitations of the approach, including its limited sensitivity and high degree of redundancy, new proteomic workflows are being developed. Among these, targeting methods in which specific peptides are selectively isolated, identified and quantified are particularly promising. Here we summarize recent incremental advances in shotgun proteomic methods and outline emerging targeted workflows. The development of the target-driven approaches with their ability to detect and quantify identical, non-redundant sets of proteins in multiple repeat analyses will be crucially important for the application of proteomics to biomarker discovery and validation, and to systems biology research.</p>},
  author       = {Malmström, Johan and Lee, Hookeun and Aebersold, Ruedi},
  issn         = {0958-1669},
  keyword      = {Mass Spectrometry,Models, Theoretical,Proteome,Proteomics,Systems Biology,Journal Article,Research Support, N.I.H., Extramural,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {4},
  pages        = {84--378},
  publisher    = {Elsevier},
  series       = {Current Opinion in Biotechnology},
  title        = {Advances in proteomic workflows for systems biology},
  url          = {http://dx.doi.org/10.1016/j.copbio.2007.07.005},
  volume       = {18},
  year         = {2007},
}