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Glucose-6-phosphate-dehydrogenase on old peroxisomes maintains self-renewal of epithelial stem cells after asymmetric cell division

Bui, Hien ; Andersson, Simon ; Sola-Carvajal, Agustin ; De Marchi, Tommaso LU ; Vähäkangas, Eliisa ; Holopainen, Minna ; House, Andrew H. ; Rovenko, Bohdana M. ; Englund, Johanna I. and Kasper, Maria , et al. (2025) In Nature Communications 16(1).
Abstract

Selective inheritance of sub-cellular components has emerged as a mechanism guiding stem cell fate after asymmetric cell divisions. Peroxisomes play a crucial role in multiple metabolic processes such as fatty acid metabolism and reactive oxygen species detoxification, but the apportioning of peroxisomes during stem cell division remains understudied. Here, we develop a mouse model and labeling technique to follow the dynamics of distinct peroxisome age-classes, and find that old peroxisomes are inherited by the daughter cell retaining full stem cell potency in mammary and epidermal stem cell divisions. Old peroxisomes carry Glucose-6-phosphate-dehydrogenase, whose specific location on the peroxisomal membrane promotes stem cell... (More)

Selective inheritance of sub-cellular components has emerged as a mechanism guiding stem cell fate after asymmetric cell divisions. Peroxisomes play a crucial role in multiple metabolic processes such as fatty acid metabolism and reactive oxygen species detoxification, but the apportioning of peroxisomes during stem cell division remains understudied. Here, we develop a mouse model and labeling technique to follow the dynamics of distinct peroxisome age-classes, and find that old peroxisomes are inherited by the daughter cell retaining full stem cell potency in mammary and epidermal stem cell divisions. Old peroxisomes carry Glucose-6-phosphate-dehydrogenase, whose specific location on the peroxisomal membrane promotes stem cell function by facilitating peroxisomal ether lipid synthesis. Our study demonstrates age-selective apportioning of peroxisomes in vivo, and unveils how functional heterogeneity of peroxisomes is utilized by asymmetrically dividing cells to metabolically divert the fate of the two daughter cells.

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publishing date
type
Contribution to journal
publication status
published
subject
in
Nature Communications
volume
16
issue
1
article number
3932
publisher
Nature Publishing Group
external identifiers
  • pmid:40287409
  • scopus:105003666433
ISSN
2041-1723
DOI
10.1038/s41467-025-58752-z
language
English
LU publication?
yes
id
e484357c-4b59-42a7-b4fb-758e44343ad2
date added to LUP
2025-07-14 11:11:30
date last changed
2025-07-15 03:00:10
@article{e484357c-4b59-42a7-b4fb-758e44343ad2,
  abstract     = {{<p>Selective inheritance of sub-cellular components has emerged as a mechanism guiding stem cell fate after asymmetric cell divisions. Peroxisomes play a crucial role in multiple metabolic processes such as fatty acid metabolism and reactive oxygen species detoxification, but the apportioning of peroxisomes during stem cell division remains understudied. Here, we develop a mouse model and labeling technique to follow the dynamics of distinct peroxisome age-classes, and find that old peroxisomes are inherited by the daughter cell retaining full stem cell potency in mammary and epidermal stem cell divisions. Old peroxisomes carry Glucose-6-phosphate-dehydrogenase, whose specific location on the peroxisomal membrane promotes stem cell function by facilitating peroxisomal ether lipid synthesis. Our study demonstrates age-selective apportioning of peroxisomes in vivo, and unveils how functional heterogeneity of peroxisomes is utilized by asymmetrically dividing cells to metabolically divert the fate of the two daughter cells.</p>}},
  author       = {{Bui, Hien and Andersson, Simon and Sola-Carvajal, Agustin and De Marchi, Tommaso and Vähäkangas, Eliisa and Holopainen, Minna and House, Andrew H. and Rovenko, Bohdana M. and Englund, Johanna I. and Kasper, Maria and Kuuluvainen, Emilia and Käkelä, Reijo and Hietakangas, Ville and Niméus, Emma and Katajisto, Pekka}},
  issn         = {{2041-1723}},
  language     = {{eng}},
  number       = {{1}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Nature Communications}},
  title        = {{Glucose-6-phosphate-dehydrogenase on old peroxisomes maintains self-renewal of epithelial stem cells after asymmetric cell division}},
  url          = {{http://dx.doi.org/10.1038/s41467-025-58752-z}},
  doi          = {{10.1038/s41467-025-58752-z}},
  volume       = {{16}},
  year         = {{2025}},
}