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Covalent attachment of actin filaments to Tween 80 coated polystyrene beads for cargo transportation

Kaur, Harsimran; Das, Tapan; Kumar, Rajesh LU ; Ajore, Ram LU and Bharadwaj, Lalit M (2008) In BioSystems 92(1). p.69-75
Abstract

In this manuscript, a new strategy has been reported for circumscribed covalent attachment of barbed and pointed ends of actin filaments to polystyrene beads. A comparative study of attachment of actin filaments to polystyrene beads was performed by blocking functionally active sites on polystyrene beads with nonionic detergents such as Tween 20, Tween 80 and polyethylene glycol (PEG). Effective blocking of active sites was obtained with Tween 80 at 0.1% concentration. Attachment of single bundle of actin filament to bead was assessed by rotational motion of bead tailed actin in front and lateral view. Velocity of actin filaments attached to different size of beads in in-vitro motility assay was calculated to ascertain their... (More)

In this manuscript, a new strategy has been reported for circumscribed covalent attachment of barbed and pointed ends of actin filaments to polystyrene beads. A comparative study of attachment of actin filaments to polystyrene beads was performed by blocking functionally active sites on polystyrene beads with nonionic detergents such as Tween 20, Tween 80 and polyethylene glycol (PEG). Effective blocking of active sites was obtained with Tween 80 at 0.1% concentration. Attachment of single bundle of actin filament to bead was assessed by rotational motion of bead tailed actin in front and lateral view. Velocity of actin filaments attached to different size of beads in in-vitro motility assay was calculated to ascertain their attachments. Velocity of actin attached to 1.0 and 3.0 microm polystyrene beads was reduced to 3.0-4.0 and 0.0-1.0 microm/s, respectively as compared to free actin velocity of 4.0-6.0 microm/s. Single point attachment of actin filaments to different size of beads was assessed by decrease in sliding velocity. Present study provides insight into the actin-myosin based molecular motor systems for drug delivery and biosensors applications.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Actins, Polysorbates, Polystyrenes, Journal Article
in
BioSystems
volume
92
issue
1
pages
7 pages
publisher
Elsevier
external identifiers
  • scopus:39949083007
ISSN
0303-2647
DOI
10.1016/j.biosystems.2007.12.003
language
English
LU publication?
no
id
e48c41ec-5ac4-4b5b-95fa-fa24c2321d60
date added to LUP
2017-07-25 11:16:15
date last changed
2017-08-27 06:45:21
@article{e48c41ec-5ac4-4b5b-95fa-fa24c2321d60,
  abstract     = {<p>In this manuscript, a new strategy has been reported for circumscribed covalent attachment of barbed and pointed ends of actin filaments to polystyrene beads. A comparative study of attachment of actin filaments to polystyrene beads was performed by blocking functionally active sites on polystyrene beads with nonionic detergents such as Tween 20, Tween 80 and polyethylene glycol (PEG). Effective blocking of active sites was obtained with Tween 80 at 0.1% concentration. Attachment of single bundle of actin filament to bead was assessed by rotational motion of bead tailed actin in front and lateral view. Velocity of actin filaments attached to different size of beads in in-vitro motility assay was calculated to ascertain their attachments. Velocity of actin attached to 1.0 and 3.0 microm polystyrene beads was reduced to 3.0-4.0 and 0.0-1.0 microm/s, respectively as compared to free actin velocity of 4.0-6.0 microm/s. Single point attachment of actin filaments to different size of beads was assessed by decrease in sliding velocity. Present study provides insight into the actin-myosin based molecular motor systems for drug delivery and biosensors applications.</p>},
  author       = {Kaur, Harsimran and Das, Tapan and Kumar, Rajesh and Ajore, Ram and Bharadwaj, Lalit M},
  issn         = {0303-2647},
  keyword      = {Actins,Polysorbates,Polystyrenes,Journal Article},
  language     = {eng},
  number       = {1},
  pages        = {69--75},
  publisher    = {Elsevier},
  series       = {BioSystems},
  title        = {Covalent attachment of actin filaments to Tween 80 coated polystyrene beads for cargo transportation},
  url          = {http://dx.doi.org/10.1016/j.biosystems.2007.12.003},
  volume       = {92},
  year         = {2008},
}