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A current view on the phenotypic antibiotic resistance of leading pathogens in wounded patients during the war in Ukraine

Kovalchuk, Valentyn ; Riesbeck, Kristian LU orcid ; Nazarchuk, Oleksandr ; Faustova, Mariia ; Dmytriiev, Dmytro ; Nazarchuk, Halyna ; Chornopyshchuk, Roman and Levchenko, Bohdan (2024) In Acta Biomedica 95(2).
Abstract

Background and aim: The purpose of this study was to determine the effectiveness of antibacterial drugs through cluster analysis of antibiotic sensitivity data from clinical isolates of Acinetobacter and Pseudomonas spp., obtained from soft tissue wounds and burn injuries. Methods: The cohort included 76 clinical isolates of Acinetobacter and Pseudomonas spp. obtained from 141 patients (male – 95,04 %; female – 4,96 %) with combined injuries of soft tissues and burns. The mean age of patients was 38,57±10,90 years. The final identification of the obtained isolates was carried using MALDI-ToF (Bruker). The sensitivity of clinical isolates to antibacterial drugs was determined using the standard Kirby-Bauer method. Extended antimicrobial... (More)

Background and aim: The purpose of this study was to determine the effectiveness of antibacterial drugs through cluster analysis of antibiotic sensitivity data from clinical isolates of Acinetobacter and Pseudomonas spp., obtained from soft tissue wounds and burn injuries. Methods: The cohort included 76 clinical isolates of Acinetobacter and Pseudomonas spp. obtained from 141 patients (male – 95,04 %; female – 4,96 %) with combined injuries of soft tissues and burns. The mean age of patients was 38,57±10,90 years. The final identification of the obtained isolates was carried using MALDI-ToF (Bruker). The sensitivity of clinical isolates to antibacterial drugs was determined using the standard Kirby-Bauer method. Extended antimicrobial susceptibility testing was performed to measure the minimal inhibitory concentrations of antibiotics against the studied. A hierarchical cluster analysis was performed using the Ward method. Results: The high level of resistance formation was observed among representatives of this Acinetobacter genus. We found the highest level of resistance in P. aeruginosa to ciprofloxacin. The lowest proportions of resistant Pseudomonas spp. were observed for meropenem and tobramycin. Cluster analysis of susceptibility data for P. aeruginosa isolates resulted in the division of antibiotics into two clusters that differ significantly from each other. The isolated A. baumannii strains showed susceptible to a higher number of antibacterial drugs compared to P. aeruginosa. Conclusions: Clinical isolates of A. baumannii and P. aeruginosa obtained from patients with combined soft tissue injuries and burns in Ukraine in 2022 exhibit a high level of multidrug resistance. Cluster analysis results indicate that only tobramycin potentially retains efficacy against A. baumannii isolates. Clinical isolates of P. aeruginosa demonstrate susceptible to tobramycin and meropenem. (www.actabiomedica.it).

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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Acinetobacter, antibiotics, multidrug resistance, Pseudomonas, resistance, sensitivity
in
Acta Biomedica
volume
95
issue
2
article number
e2024030
publisher
Mattioli 1885
external identifiers
  • scopus:85192365829
ISSN
0392-4203
DOI
10.23750/abm.v95i2.15395
language
English
LU publication?
yes
id
e4a9308b-c9c9-4cd9-aab6-77143f0652c0
date added to LUP
2025-01-10 12:24:56
date last changed
2025-04-04 14:36:18
@article{e4a9308b-c9c9-4cd9-aab6-77143f0652c0,
  abstract     = {{<p>Background and aim: The purpose of this study was to determine the effectiveness of antibacterial drugs through cluster analysis of antibiotic sensitivity data from clinical isolates of Acinetobacter and Pseudomonas spp., obtained from soft tissue wounds and burn injuries. Methods: The cohort included 76 clinical isolates of Acinetobacter and Pseudomonas spp. obtained from 141 patients (male – 95,04 %; female – 4,96 %) with combined injuries of soft tissues and burns. The mean age of patients was 38,57±10,90 years. The final identification of the obtained isolates was carried using MALDI-ToF (Bruker). The sensitivity of clinical isolates to antibacterial drugs was determined using the standard Kirby-Bauer method. Extended antimicrobial susceptibility testing was performed to measure the minimal inhibitory concentrations of antibiotics against the studied. A hierarchical cluster analysis was performed using the Ward method. Results: The high level of resistance formation was observed among representatives of this Acinetobacter genus. We found the highest level of resistance in P. aeruginosa to ciprofloxacin. The lowest proportions of resistant Pseudomonas spp. were observed for meropenem and tobramycin. Cluster analysis of susceptibility data for P. aeruginosa isolates resulted in the division of antibiotics into two clusters that differ significantly from each other. The isolated A. baumannii strains showed susceptible to a higher number of antibacterial drugs compared to P. aeruginosa. Conclusions: Clinical isolates of A. baumannii and P. aeruginosa obtained from patients with combined soft tissue injuries and burns in Ukraine in 2022 exhibit a high level of multidrug resistance. Cluster analysis results indicate that only tobramycin potentially retains efficacy against A. baumannii isolates. Clinical isolates of P. aeruginosa demonstrate susceptible to tobramycin and meropenem. (www.actabiomedica.it).</p>}},
  author       = {{Kovalchuk, Valentyn and Riesbeck, Kristian and Nazarchuk, Oleksandr and Faustova, Mariia and Dmytriiev, Dmytro and Nazarchuk, Halyna and Chornopyshchuk, Roman and Levchenko, Bohdan}},
  issn         = {{0392-4203}},
  keywords     = {{Acinetobacter; antibiotics; multidrug resistance; Pseudomonas; resistance; sensitivity}},
  language     = {{eng}},
  number       = {{2}},
  publisher    = {{Mattioli 1885}},
  series       = {{Acta Biomedica}},
  title        = {{A current view on the phenotypic antibiotic resistance of leading pathogens in wounded patients during the war in Ukraine}},
  url          = {{http://dx.doi.org/10.23750/abm.v95i2.15395}},
  doi          = {{10.23750/abm.v95i2.15395}},
  volume       = {{95}},
  year         = {{2024}},
}