Polyclonal expansion of cells with trisomy 7 in synovia from patients with osteoarthritis
(1998) In Cytogenetics and Cell Genetics 83(1-2). p.4-30- Abstract
Trisomy 7 as the single chromosome aberration has been found in a variety of neoplasms and in normal tissue in the proximity of tumors, as well as in non-neoplastic lesions. Recently, we described a nonrandom pattern of chromosome aberrations, in particular, a gain of chromosome 7, in synovia, cartilage, and osteophytes from patients with osteoarthritis. To study the clonal origin of trisomy 7 in osteoarthritis, multiple synovial samples were collected from five women, all of whom were informative heterozygotes with regard to the X-linked human androgen receptor gene (AR). From each case, three to four independent cell cultures were initiated. Trisomic cell populations were subcloned from the individual cultures, and it was established... (More)
Trisomy 7 as the single chromosome aberration has been found in a variety of neoplasms and in normal tissue in the proximity of tumors, as well as in non-neoplastic lesions. Recently, we described a nonrandom pattern of chromosome aberrations, in particular, a gain of chromosome 7, in synovia, cartilage, and osteophytes from patients with osteoarthritis. To study the clonal origin of trisomy 7 in osteoarthritis, multiple synovial samples were collected from five women, all of whom were informative heterozygotes with regard to the X-linked human androgen receptor gene (AR). From each case, three to four independent cell cultures were initiated. Trisomic cell populations were subcloned from the individual cultures, and it was established whether or not the same allele of AR was inactivated in trisomic cells from different parts of the same joint. The finding of a polyclonal X-inactivation pattern in two of the cases provides strong evidence that gain of an extra copy of chromosome 7 occurs independently in multiple cells.
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- author
- Broberg, K LU ; Höglund, M LU ; Lindstrand, A LU ; Toksvig-Larsen, S LU ; Mandahl, N LU and Mertens, F LU
- publishing date
- 1998
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Aged, Biopsy, Chromosome Aberrations, Chromosomes, Human, Pair 7, Cloning, Molecular, Dosage Compensation, Genetic, Female, Humans, In Situ Hybridization, Fluorescence, Karyotyping, Osteoarthritis/genetics, Synovial Membrane/pathology, Trisomy/genetics
- in
- Cytogenetics and Cell Genetics
- volume
- 83
- issue
- 1-2
- pages
- 4 - 30
- publisher
- Karger
- external identifiers
-
- pmid:9925917
- scopus:0032421556
- ISSN
- 0301-0171
- DOI
- 10.1159/000015160
- language
- English
- LU publication?
- no
- id
- e5cce63b-2561-4d4e-884d-6809b95c3f6b
- date added to LUP
- 2019-02-12 15:53:05
- date last changed
- 2024-01-15 14:19:07
@article{e5cce63b-2561-4d4e-884d-6809b95c3f6b, abstract = {{<p>Trisomy 7 as the single chromosome aberration has been found in a variety of neoplasms and in normal tissue in the proximity of tumors, as well as in non-neoplastic lesions. Recently, we described a nonrandom pattern of chromosome aberrations, in particular, a gain of chromosome 7, in synovia, cartilage, and osteophytes from patients with osteoarthritis. To study the clonal origin of trisomy 7 in osteoarthritis, multiple synovial samples were collected from five women, all of whom were informative heterozygotes with regard to the X-linked human androgen receptor gene (AR). From each case, three to four independent cell cultures were initiated. Trisomic cell populations were subcloned from the individual cultures, and it was established whether or not the same allele of AR was inactivated in trisomic cells from different parts of the same joint. The finding of a polyclonal X-inactivation pattern in two of the cases provides strong evidence that gain of an extra copy of chromosome 7 occurs independently in multiple cells.</p>}}, author = {{Broberg, K and Höglund, M and Lindstrand, A and Toksvig-Larsen, S and Mandahl, N and Mertens, F}}, issn = {{0301-0171}}, keywords = {{Aged; Biopsy; Chromosome Aberrations; Chromosomes, Human, Pair 7; Cloning, Molecular; Dosage Compensation, Genetic; Female; Humans; In Situ Hybridization, Fluorescence; Karyotyping; Osteoarthritis/genetics; Synovial Membrane/pathology; Trisomy/genetics}}, language = {{eng}}, number = {{1-2}}, pages = {{4--30}}, publisher = {{Karger}}, series = {{Cytogenetics and Cell Genetics}}, title = {{Polyclonal expansion of cells with trisomy 7 in synovia from patients with osteoarthritis}}, url = {{http://dx.doi.org/10.1159/000015160}}, doi = {{10.1159/000015160}}, volume = {{83}}, year = {{1998}}, }