Advanced

Polyclonal expansion of cells with trisomy 7 in synovia from patients with osteoarthritis

Broberg, K LU ; Höglund, M LU ; Lindstrand, A LU ; Toksvig-Larsen, S LU ; Mandahl, N LU and Mertens, F LU (1998) In Cytogenetics and Cell Genetics1973-01-01+01:002002-01-01+01:00 83(1-2). p.4-30
Abstract

Trisomy 7 as the single chromosome aberration has been found in a variety of neoplasms and in normal tissue in the proximity of tumors, as well as in non-neoplastic lesions. Recently, we described a nonrandom pattern of chromosome aberrations, in particular, a gain of chromosome 7, in synovia, cartilage, and osteophytes from patients with osteoarthritis. To study the clonal origin of trisomy 7 in osteoarthritis, multiple synovial samples were collected from five women, all of whom were informative heterozygotes with regard to the X-linked human androgen receptor gene (AR). From each case, three to four independent cell cultures were initiated. Trisomic cell populations were subcloned from the individual cultures, and it was established... (More)

Trisomy 7 as the single chromosome aberration has been found in a variety of neoplasms and in normal tissue in the proximity of tumors, as well as in non-neoplastic lesions. Recently, we described a nonrandom pattern of chromosome aberrations, in particular, a gain of chromosome 7, in synovia, cartilage, and osteophytes from patients with osteoarthritis. To study the clonal origin of trisomy 7 in osteoarthritis, multiple synovial samples were collected from five women, all of whom were informative heterozygotes with regard to the X-linked human androgen receptor gene (AR). From each case, three to four independent cell cultures were initiated. Trisomic cell populations were subcloned from the individual cultures, and it was established whether or not the same allele of AR was inactivated in trisomic cells from different parts of the same joint. The finding of a polyclonal X-inactivation pattern in two of the cases provides strong evidence that gain of an extra copy of chromosome 7 occurs independently in multiple cells.

(Less)
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Aged, Biopsy, Chromosome Aberrations, Chromosomes, Human, Pair 7, Cloning, Molecular, Dosage Compensation, Genetic, Female, Humans, In Situ Hybridization, Fluorescence, Karyotyping, Osteoarthritis/genetics, Synovial Membrane/pathology, Trisomy/genetics
in
Cytogenetics and Cell Genetics1973-01-01+01:002002-01-01+01:00
volume
83
issue
1-2
pages
4 - 30
publisher
Karger
external identifiers
  • scopus:0032421556
ISSN
0301-0171
DOI
10.1159/000015160
language
English
LU publication?
no
id
e5cce63b-2561-4d4e-884d-6809b95c3f6b
date added to LUP
2019-02-12 15:53:05
date last changed
2019-02-20 16:38:43
@article{e5cce63b-2561-4d4e-884d-6809b95c3f6b,
  abstract     = {<p>Trisomy 7 as the single chromosome aberration has been found in a variety of neoplasms and in normal tissue in the proximity of tumors, as well as in non-neoplastic lesions. Recently, we described a nonrandom pattern of chromosome aberrations, in particular, a gain of chromosome 7, in synovia, cartilage, and osteophytes from patients with osteoarthritis. To study the clonal origin of trisomy 7 in osteoarthritis, multiple synovial samples were collected from five women, all of whom were informative heterozygotes with regard to the X-linked human androgen receptor gene (AR). From each case, three to four independent cell cultures were initiated. Trisomic cell populations were subcloned from the individual cultures, and it was established whether or not the same allele of AR was inactivated in trisomic cells from different parts of the same joint. The finding of a polyclonal X-inactivation pattern in two of the cases provides strong evidence that gain of an extra copy of chromosome 7 occurs independently in multiple cells.</p>},
  author       = {Broberg, K and Höglund, M and Lindstrand, A and Toksvig-Larsen, S and Mandahl, N and Mertens, F},
  issn         = {0301-0171},
  keyword      = {Aged,Biopsy,Chromosome Aberrations,Chromosomes, Human, Pair 7,Cloning, Molecular,Dosage Compensation, Genetic,Female,Humans,In Situ Hybridization, Fluorescence,Karyotyping,Osteoarthritis/genetics,Synovial Membrane/pathology,Trisomy/genetics},
  language     = {eng},
  number       = {1-2},
  pages        = {4--30},
  publisher    = {Karger},
  series       = {Cytogenetics and Cell Genetics1973-01-01+01:002002-01-01+01:00},
  title        = {Polyclonal expansion of cells with trisomy 7 in synovia from patients with osteoarthritis},
  url          = {http://dx.doi.org/10.1159/000015160},
  volume       = {83},
  year         = {1998},
}